Maximizing content of Omega-3 (EPA and DHA) in the process of enzymatic acidolysis of canola oil and concentrated of long-chain polyunsaturated fatty acids (LCPUFA) in supercritical CO2 conditions

Authors

  • José Cedano Universidad de Chile, Santiago de Chile
  • Alicia Rodríguez Universidad de Chile, Santiago de Chile
  • Raúl Siche Universidad Nacional de Trujillo, Trujillo

DOI:

https://doi.org/10.17268/sci.agropecu.2015.04.08

Keywords:

Optimization, supercritical carbon dioxide, structured triacylglycerols, docosahexaenoic acid (DHA), eicosapentaenoico acid (EPA)

Abstract

The aim of this study was to optimize the content of EPA and DHA in the process of enzymatic acidolysis of canola oil and concentrated of long-chain polyunsaturated fatty acids (LCPUFA) in structured triacylglycerols (TAGs). For this purpose, nonspecific lipase B from Candida antarctica immobilized in a supercritical CO2 was used. Crude salmon oil obtained from the industrial byproducts was used to obtain LCPUFA concentrate. Initially, a LCPUFAs concentrate was obtained by basic hydrolysis and posterior complexation with urea. Subsequently the process variables were optimized enzymatic acidolysis were optimized using a central composite rotational design 25-1 + star, with 5 factors and 30 experimental trials, based on the response surface methodology. The optimal conditions that maximized the content of EPA and DHA to 3.92 g/100 g TFA and 9.09 g/100 g TFA, respectively in the purified TAGs corresponded to a LCPUFA percentage 71.71% and canola oil percentage 28.29%, temperature 57.8 °C, pressure 172.0 bar, time 23.97 h enzyme percentage of 7.74%.

References

AOCS. 1993. Official Methods and Recommended Practices of American Oil Chemists’ Society. 4th Ed. AOCSS Press, Champaign: Ca 5a-40:1, Cd 8b-90: 1 – 2, Cd 18-19: 1 – 2.

AOCS. 2009. Determination of cis-, trans-, saturated, mono-unsaturated, and polyunsaturated fatty acids in extracted fats by capillary GLC. AOCS Official Method Ce 1j-7. Sampling and analysis of commercial fats and oils.

Araya, J. 2008. Riesgos y beneficios del consumo de grasas y aceites. En Programa de Educación a Distancia. Departamento de Nutrición. Facultad de Medicina. Universidad de Chile.

Burr, M.L.; Fehily, A.M.; Gilbert, J.F.; Rogers, S.; Holliday, R.M.; Sweetnam, P.M.; Elwood, P.C.; Deadman, N.M. 1989. Effects of changes in fat, fish, and fibre intakes on death and myocardial reinfarction: diet and reinfarction trial (DART). Lancet 2(8666): 757- 761.

CRN (Council For Responsible Nutrition). 2015. Oxidation in Omega-3 Oils: An Overview. A White Paper Prepared by the Global Organization for EPA and DHA Omega-3s. Available in: http://crnusa.org/pdfs/GOED+CRNWhitePaper-Omega-3oxidation.pdf

Dyerberg, J.; Madsen, P.; Møller, J.M.; Aardestrup, I.; Schmidt, E.B. 2010. Biovailabity of marine n-3 fatty acid formulations. Prostaglandins, Leukotrienes and Essential Fatty Acids 83: 137-141.

FAO (Organización de las Naciones Unidas para la alimentación y la agricultura). 2012. Grasas y ácidos grasos en nutrición humana. Consulta de expertos. Estudio FAO Alimentación y Nutrición ISSN 1014-2916 FAO ISBN 978-92-5-3067336. FAO y FINUT, 2012 (edición española).

Ghazani, S.M.; Marangoni, A.G. 2013. Minor components in canola oil and effects of refining on these constituents: A review. Journal of the American Oil Chemists’ Society 90: 923 – 932.

Haggsma, N.; Van Gent, C.M.; Luten, J.B.; De Jong, R.W.; Van Doorn, E. 1982. Preparation of an ω-3 fatty acid concentrate from cod liver oil. Journal of the American Oil Chemists’ Society 59(3): 117 – 118.

Huang, K.H.; Akoh, C.C. 1996. Optimization and scale up of enzymatic synthesis of structure lipids using RSM. J Food Sci 6:137–41.

IFOS (International Fish Oil Standards). 2009. Consumer Report. Available in: http://www.nutrasource.ca/ifos/

Iverson, J.L.; Weik, R.W. 1967. Correlation of fatty acid structure with preferential order of urea complex formation. Journal of the Association of Official Analytical Chemists’ 50: 1111 – 118.

Jiménez, M.J.; Esteban, L.; Robles, A.; Hita, E.; González, P.A.; Muñío, M.M.; Molina, E. 2010. Production of triacylglycerols rich in palmitic acid at sn-2 position by lipase-catalyzed acidolysis. Biochemical Engineering Journal 51: 172-179.

Liu, S.; Zhang, C.; Hong, P.; Ji, H. 2006. Concentration of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) of tuna oil by urea complexation: optimization of process parameters. Journal of Food Engineering 73: 203 – 209.

Masson, L. 1994. Criterio de calidad para materias grasas utilizadas frecuentemente en la nutrición animal y de peces. Available in: http://www.fao.org/docrep/field/003/ab482s/ab482s10.htm.

Masson, L.; Mella, M. 1985. Materias grasas de consumo habitual y potencial en Chile: Composición en ácidos grasos. 1ª ed, Editorial Universitaria, Santiago, Chile. 30 p.

Méndez, C.; Masson, L.; Jiménez, P. 2010. Estabilización de aceite de pescado por medio de antioxidantes naturales. A&G 30(3): 270 – 278.

Neubronner, J.; Schuchardt, J.P.; Kressel, G.; Merkel, M.; Von, Schacky C.; Hahn, A. 2011. Enhanced increase of omega-3 index in response to long-term n-3 fatty acid supplementation from triacylglycerides versus ethyl esters. European Journal of Clinical Nutrition 65: 247-254.

Neuringer, M.; Connor, W.E.; Lin, D.S.; Barstad, L.; Luck, S. 1986. Biochemical and functional effects of prenatal and postnatal omega 3 fatty acid deficiency on retina and brain in rhesus monkeys. Proc. Nat. Acad. Sci. 83: 4021-4025.

Osborn, H.T.; Akoh, C.C. 2002. Structured lipids-novel fats with medical, nutraceutical, and food applications. Comprehensive reviews in Food Science and Food Safety 45: 110-120.

Pando, M.E.; Bravo, B.; Berrios, M.; Galdames, A.; Rojas, C.; Romero, N.; Camilo, C.; Encina, C.; Rivera, M.; Rodriguez, A.; Aubourg, S. 2014. Concentrating n-3 fatty acids from crude and refined commercial salmon oil. Czech J. Food Sci 32(2): 169 – 176.

Ramírez, A. 2006. Salmon by-products proteins Circular área marina Nº 1027 FAO, Roma, Italia.

Ratnayake, W.M.N.; Olsson, B.; Matthews, D.; Ackman, R.G. 1988. Preparation of omega-3 PUFA concentrates from fish oils via urea complexation. Fat Science and Technology 90: 381 – 386.

Robles, A.; Esteban, L.; Giménez, A.; Camacho, B.; Ibañez, M.J.; Molina, E. 1999. Lipase-catalyzed esterification of glycerol and polyunsaturated fatty acids from fish and microalgae oils. Journal of Biotechnology 70: 379 – 391.

Robles, A.; Jiménez, M.J.; Esteban, L.; Gonzáles, P.A.; Martín, L.; Rodríguez, A.; Molina, E. 2011. Enzymatic production of human milk fat substitutes containing palmitic and docosahexaenoic acids at sn-2 position and oleic acid at sn-1,3 positions. LWT-Food Science and Technology 44: 1986-1992.

Sharma, M.; Rastogi, N.K.; Lokesk, B.R. 2009. Synthesis of structured lipid with balanced Omega-3: Omega-6 ratio by lipase-catalyzed acidolysis reaction: Optimization of reaction using response surface methodology. Process Biochemistry 44: 1284 – 1288.

Simopoulos, A.P. 2002. The importance of the ratio of omega-6/omega-3 essential fatty acids. Biomed Pharmacother 56: 365 – 379.

Simopoulus, A. 1991. Omega-3 fatty acids in health and disease and in grown and development. Am. J. Clin. Nutr. 54: 438-468.

Strocchi, A.; Bonaga, G. 1975. Correlation between urea inclusion compounds and conformational structure of unsaturated C-18 fatty acid methyl esters. Chemical Physical Lipids 15: 87 – 94.

Uauy, R.; Valenzuela, A. 2000. Marine Oils: The health benefits of n-3 fatty acids. Nutrition 16: 680-684.

Uauy-Dagach, R.; Valenzuela, A. 1992. Marine oils as a source of Omega-3 fatty acids in the diet. Prog. Food Nutr. Sci. 16:199–243.

Valenzuela, A.; Sanhueza, J. 2009. Aceites de origen marino; Su importancia en la nutrición y en la ciencia de los alimentos. Revista Chilena de Nutrición 36 (3): 246 – 257.

Valenzuela, R.; Tapia, G.; González, M.; Valenzuela, A. 2011. Ácidos grasos omega-3 (EPA y DHA) y sus aplicaciones en diversas situaciones clínicas. Revista Chilena de Nutrición 38 (3): 356 – 367.

Wanasundara, U.A.; Shahidi, F. 1995. Storage stability of microencapsulated seal blubber oil. J. Food Lipids 2: 73 – 86.

Wanasundara, U.N. 1996. Marine oils: stabilization, structural characterization and omega-3 fatty acid concentration. Ph.D. thesis, Memorial University of Newfoundland, Canada.

Wanasundara, U.; Shahidi, F. 1999. Concentration of omega 3-polyunsaturated fatty acids of seal blubber oil by urea complexation: optimization of reaction conditions. Food Chemistry 65:41-49.

Recibido 08 julio 2015.

Aceptado 06 noviembre 2015.

Corresponding author: E-mail: rsiche@unitru.edu.pe (R. Siche).

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Published

2016-12-05

How to Cite

Cedano, J., Rodríguez, A., & Siche, R. (2016). Maximizing content of Omega-3 (EPA and DHA) in the process of enzymatic acidolysis of canola oil and concentrated of long-chain polyunsaturated fatty acids (LCPUFA) in supercritical CO2 conditions. Scientia Agropecuaria, 6(4), 313-323. https://doi.org/10.17268/sci.agropecu.2015.04.08

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