Kinetics of color development in glucose/Amino Acid model systems at different temperatures


  • Ana Echavarría Universidad Estatal de Milagro (UNEMI), Guayas
  • Jordi Pagán Universidad de Lleida, Lleida
  • Albert Ibarz Universidad de Lleida, Lleida


Palabras clave:

antioxidant activity, phenolic compounds, residue flour, DPPH assay


This study investigated the influence of temperature on the color development of melanoidins formed from a single combination of glucose with amino acid. The selected amino acid, commonly found in apple juice and highly reactive in the Maillard reaction, were asparagine (Asn), aspartic acid (Asp) and glutamic acid (Glu). For this, the color development was evaluated by measuring browning at 420 nm and color measurements by spectrophotometry and colorimetry methods. The effect of temperature on the color intensity, the absorption of melanoidins were also measured at different wavelengths (280, 325, 405). The value of melanoidins formed from all model systems was located on a dominant wavelength of 325 nm, the ultra violet zone of the diagram. A first-order kinetic model was applied to L* and the evolution of color difference ΔE*. In addition, a*, b* values, significantly differences were found in the glucose/aspartic acid model system in the brown-red zone. Therefore, the color development of the melanoidins was influenced by the type of amino acid and temperature, and it is thought that the a* and b* values can be used to explain the differences among the amino acid in the color development of melanoidins.


Bharate, S.S.; Bharate, S.B. 2014. Non-enzymatic browning in citrus juice: chemical markers, their detection and ways to improve product quality 51(10): 2271–2288.

Billaud, C.; Maraschin, C.; Nicolas, J. 2004. Inhibition of polyphenoloxidase from apple by Maillard reaction products prepared from glucose or fructose with L-cysteine under various conditions of pH and temperature. LWT Food Science and Technology 37: 69-78.

Carabasa-Giribet, M.; Ibarz, A. 2000. Kinetics of colour development in aqueous fructose systems at high temperatures. Journal of the Science of Food and Agriculture 80(14): 2105-2113.

Contreras-Calderón, J.; Guerra-Hernández, E.; García-Villanova, B. 2009. Utility of some indicators related to the Maillard browning reaction during processing of infant formulas. Food Chemistry 114: 1265-1270.

Demirhan, E.; Özbek, B. 2009. Color Change Kinetics of Microwave-Dried Basil. Drying Technology 27: 156-166.

Echavarría, A.P.; Pagán, J.; Ibarz, A. 2013. Optimization of Maillard reaction products isolated from sugar-amino acid model system and their antioxidant activity. Afinidad 70(562): 86-92.

Echavarría, A.P.; Pagán, J.; Ibarz, A. 2012. Melanoidins Formed by Maillard Reaction in Food and Their Biological Activity. Food Engineering Review 4: 203-223.

Echavarría, A.P.; Torras, C.; Pagán, J.; Ibarz, A. 2011. Fruit juice processing and membrane technology application. Food Engineering Review 3: 136-158.

Ganjloo, A.; Rahman, R.; Bakar, J.; Osman, A.; Bimakr, M. 2009. Modelling the kinetics of peroxidase inactivation and colour changes of seedless guava (Psidium guajava L) during thermal treatments. World Applied Sciences Journal 7 (1): 105-112.

Gu, F.; Kim, J.; Abbas, S.; Zhang. X.; Xia, S.; Chen, Z. 2010. Structure and antioxidant activity of high molecular weight Maillard reaction products from casein-glucose. Food Chemistry 120: 505-511.

Ibarz, A.; Garza S.; Pagán, J. 2008. Inhibitory effect of melanoidins from glucose-asparagine on carboxypeptidases activity. European Food Research and Technology 226: 1277-1282.

Ibarz, A.; Pagán, J.; Garza, S. 2000. Kinetic models of non-enzymatic browning in apple puree. Journal of the Science of Food and Agriculture 80: 1162-1168.

Jaeger, H.; Janositz A.; Knorr, D. 2010. The Maillard reaction and its control during food processing. The potential of emerging technologies. Pathologie Biologie 58(3): 207-13.

Kim, J.; Lee, Y. 2008. The Influence of pH on the color development of melanoidins formed from fructose/amino acid enantiomer model Systems. Journal of Food science and nutrition 13: 306-312.

Kim, J.; Ra, K.; Suh, H. 2004. Hydrolysis of onion and kinetics of non-enzymatic browning of its hydrolysate. International Journal of Food Science & Technology 10(1): 41-44.

Morales, F.; Jiménez-Pérez, S. 2001. Free radical scavenging of Maillard reaction products as related to color and fluorescence. Food Chemistry 72: 119-125.

Rattanathanalerk, M.; Chiewchan, N.; Srichumpoung, W. 2005. Effect of thermal processing on the quality loss of pineapple juice. Journal of Food Engineering 66: 259-265.

Rufián-Henares, J.; Morales, F. 2007. Functional properties of melanoidins: In vitro antioxidant, antimicrobial and antihypertensive activities. Food Research International 40(8): 995-1002.

Vaikousi, H.; Koutsoumanis, K.; Biliaderis, C. 2008. Kinetic modelling of non-enzymatic browning of apple juice concentrates differing in water activity under isothermal and dynamic heating conditions. Food Chemistry 107: 785-796.

Valdramidis, V.; Cullen, P.; Tiwari, B.; O´donnell, C. 2010. Quantitative modelling approaches for ascorbic acid degradation and non-enzymatic browning of orange juice during ultrasound processing. Journal of Food Engineering 96(3): 449-45.

Zyzelewicz, D.; Kryslak, W.; Nebesny, E.; Budryn, G. 2014. Application of various methods for determination of the color of cocoa beans roasted under variable process parameter European Food Research and Technology 238: 549–563.

Received August 22, 2015.

Accepted January 20, 2016.

* Corresponding author

E-mail: (A.P. Echavarría).



Cómo citar

Echavarría, A., Pagán, J., & Ibarz, A. (2016). Kinetics of color development in glucose/Amino Acid model systems at different temperatures. Scientia Agropecuaria, 7(1), 15-21.



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