Prospecting and management alternatives of fungal diseases associated in the blueberry crop (Vaccinium corymbosum L.)

Authors

  • René Aguilar-Anccota Universidad Nacional de Piura, Campus Universitario s/n. Urb. Miraflores, Piura
  • Miguel Á. Imán-Castillo Universidad Nacional de Piura, Campus Universitario s/n. Urb. Miraflores, Piura
  • José A. Silupú-Masías Universidad Nacional de Piura, Campus Universitario s/n. Urb. Miraflores, Piura
  • Robert Rafael-Rutte Universidad Nacional Tecnológica de Lima Sur, Sector 3 Grupo 1A 03, Av. Central y Av. Bolivar, Villa El Salvador, Lima
  • Yuri H. Calle-Cheje Universidad Católica Sedes Sapientiae, Campus Km 159 Panamericana Norte - Mazo Huaura. Lima
  • Ivis Andrei Campos e Silva Universidade Federal Rural de Pernambuco. Rua Dom Manuel de Medeiros, s/n, Dois Irmãos - CEP: 52171-900 - Recife/PE, Pernambuco
  • Arturo Morales-Pizarro Universidad Nacional de Piura, Campus Universitario s/n. Urb. Miraflores, Piura
  • Edgar Maldonado Duque Universidad Nacional de Piura, Campus Universitario s/n. Urb. Miraflores, Piura
  • Javier Javier Alva Universidad Nacional de Piura, Campus Universitario s/n. Urb. Miraflores, Piura

DOI:

https://doi.org/10.17268/agroind.sci.2023.03.07

Keywords:

Vaccinium corymbosum L., Pestalotia sp., Lasiodiplodia theobromae, Thekopsora minima, Botrytis cinerea

Abstract

The cultivation area of blueberries (Vaccinium corymbosum L.) has a significant growth worldwide due to its nutritional value, the growing global demand and its economic potential. In Peru, the production of this crop is mainly for export and one of the main producing areas is the province of Trujillo. The objective of the study was to characterize and describe the symptoms of pathogens causing fungal diseases in the blueberry crop. Samples were taken from plants showing necrotic streaks on the stem, leaves and fruit for microbiological analysis in the laboratory. Samples were processed by direct observation of symptoms and presence of signs, using a humid chamber to promote sporulation and PDA culture medium for fungal isolates. Pestalotia sp. causing leaf spot and stem blight, Thekopsora minima causing leaf rust, Botrytis cinerea causing gray mold on flowers and fruit, and Lasiodiplodia theobromae causing dieback were identified. To corroborate their identification, pathogenicity tests were performed. For the control of these diseases, it is recommended that an integrated management plan be implemented to reduce the conditions for pathogen development, thus avoiding short-, medium- and long-term economic losses in the blueberry crop.

References

Amézquita, Z. G. (2022). Manejo integrado de plagas en el cultivo de arándano (Vaccinium corymbosum L.) bajo condiciones del valle de Huarmey Áncash. https://hdl.handle.net/20.500.12996/5574

ANEBERRIES. Asociación Nacional de Exportadores de Berries. Lista de plaguicidas autorizados 2016. [Consultado el 23 de julio de 2023]. Disponible en: https://berriesdemexico.com/

Apaza, W. (2021). Manejo Integrado de Lasiodiplodia theobromae en arándanos en la irrigación de Chavimochic. Boletín técnico Nº 5. Junta de usuarios de riego presurizado del Distrito de riego Moche Virú y Chao. 11 p. http://funnels.novagro-ag.com/lnd-lasiodiplodia-en-arandano/

Barnett, H. L. & Hunter, B. B. (1998). Illustrated genera of imperfect fungi. McMillan Publising Company. 4, th Ed. New York. 218 p.

Bautista, M. J. I. (2020). Descripción del manejo agronómico del cultivo de arándanos (Vaccinium corymbosum L.) variedades biloxy, legacy en el municipio de Mutiscua Departamento de Norte de Santander. http://repositoriodspace.unipamplona.edu.co/jspui/handle/20.500.12744/2107

Bello, F., Montironi, I. D., Medina, M. B., Munitz, M. S., Ferreira, F. V., Williman, C., Vázquez, D., Cariddi, L. N., & Musumeci, M. A. (2022). Mycofumigation of postharvest blueberries with volatile compounds from Trichoderma atroviride IC-11 is a promising tool to control rots caused by Botrytis cinerea. Food Microbiology, 106, 104040. https://doi.org/10.1016/j.fm.2022.104040

Carlier, E., Cabrera, C., & Zapata, L. M. (2021). Mineral composition of blueberries (Vaccinium corymbosum) cultivated in the northeast region of Argentina. Revista Iberoamericana de Tecnología Postcosecha, 22(1). https://www.redalyc.org/journal/813/81367929007/81367929007.pdf

Caruso, F. L. & Ramsdell, D. C. (1995). Compendium of Blueberry and Cranberry Diseases. St. Paul, MN, APS. 87 p. https://books.google.com.pe/books/about/Compendium_of_Blueberry_and_Cranberry_Di.html?id=EpOimwEACAAJ&redir_esc=y

Estacio-Yalan, C. I. (2019). Identificación de especies fúngicas asociadas al decaimiento de plantas en el cultivo de arándano (Vaccinium corymbosum L.) en la Región Ica y el valle de Cañete. https://hdl.handle.net/20.500.13028/3171

Fang, Y., Nunez, G., Silva, M., et al. (2020). A review for southern highbush blueberry alternative production systems. Agronomy, 10(1531), 1-15. https://doi.org/10.3390/agronomy10101531

García, G. 2010. Guía de cultivo orientaciones para el cultivo del arándano. Proyecto de cooperación “Nuevos Horizontes”. Ministerio de medio ambiente y medio rural y marino. España. pp. 32

Ghezzi, P. & Stein, E. H. (2021). Los arándanos en el Perú. Banco Iberoamericano de Desarrollo, NY. 35 pp.

Gonza, M. E. (2013). Prospección de las principales enfermedades fungosas en el cultivo de cafeto (Coffea arabica L.), en el distrito de Canchaque-Alto Piura. Tesis para optar el título de Ingeniero Agrónomo. Universidad Nacional de Piura. Repositorio de tesis digitales UNP. http://repositorio.unp.edu.pe/handle/20.500.12676/3637

Huang, X., Ren, J., Li, P., Feng, S., Dong, P. & Ren, M. (2021). Potential of microbial endophytes to enhance the resistance to postharvest diseases of fruit and vegetables. Journal of the Science of Food and Agriculture, 101(5), 1744-1757. https://doi.org/10.1002/jsfa.1082

Huarhua, M., Acuña, R., Aragón, L., Soto, J., Landeo, S., Martínez de la Parte, E., & Apaza, W. (2020). First report of blueberry leaf rust caused by Thekopsora minima on Vaccinium corymbosum in Peru. Plant Disease, 104(11), 3077-3077. https://doi.org/10.1094/PDIS-03-20-0585-PDN

Isaza, L., Zuluaga, Y. P. & Marulanda, M. L. (2019). Morphological, pathogenic and genetic diversity of Botrytis cinerea Pers. in blackberry cultivations in Colombia. Revista Brasileira de Fruticultura, 41. https://doi.org/10.1590/0100-2945201949

Jiang, B., Liu, R., Fang, X., Wu, W., Han, Y., Chen, H., ... & Gao, H. (2022). Botrytis cinerea infection affects wax composition, content and gene expression in blueberry fruit. Postharvest biology and technology, 192, 112020. https://doi.org/10.1016/j.postharvbio.2022.112020

Keith, L., Sugiyama, L., Strauss, A., Kai, R., Zee, F., Hamasaki, R., ... & Nakamoto, S. (2008). First report of leaf rust of blueberry caused by Pucciniastrum vaccinii in Hawaii. Plant Disease, 92(11), 1590-1590. https://doi.org/10.1094/PDIS-92-11-1590A

Kurtzman, C. P. & Droby, S. (2001) Metschnikowia fructicola, a new ascosporic yeast with potencial for biocontrol of postharvest fruits rot. Syst. Appl. Microbiol., 24, 395-399. https://pubag.nal.usda.gov/download/24599/pdf

Latham, R. L, Beal, E. J., Clarkson, J. P. & Nellist, C. F. (2022) First report of Pucciniastrum minimum (syn. Thekopsora minima) causing leaf rust on Vaccinium corymbosum (blueberry) in the United Kingdom and pathogenicity on Vaccinium myrtillus (bilberry). New Dis Rep., 45(1): e12057. https://doi.org/10.1002/ndr2.12057

Li, X., Leng, J., Yu, L., Bai, H., Li, X., Wisniewski, M., Liu, J., & Sui, Y. (2022). Efficacy of the biocontrol agent Trichoderma hamatum against Lasiodiplodia theobromae on macadamia. Frontiers in microbiology, 13, 994422. https://doi.org/10.3389/fmicb.2022.994422

Liu, B., Hu, T. & Yan, W. (2020). Authentication of the bilberry extracts by an HPLC fingerprint method combining reference standard extracts. Molecules, 25, 2514. https://doi.org/10.3390%2Fmolecules25112514

Lorrain, C., Gonçalves Dos Santos, K. C., Germain, H., et al. (2019). Advances in understanding obligate biotrophy in rust fungi. New Phytologist, 222(3), 1190-1206. https://doi.org/10.1111/nph.15641

Mctaggart, A. R., Geering, A. D. W., & Shivas, R. G. (2013). Thekopsora minima causes blueberry rust in south-eastern Queensland and northern New South Wales. Australasian Plant Disease Notes, 8, 81-83. https://doi.org/10.1007/s13314-013-0101-2

Mohali, S., Burgess, T. I., & Wingfield, M. J. (2005). Diversity and host association of the tropical tree endophyte Lasiodiplodia theobromae revealed using simple sequence repeat markers. Forest Pathology, 35(6), 385-396. https://doi.org/10.1111/j.1439-0329.2005.00418.x

Morales, L. A., Martínez, S. J., Mavd, I., et al. (2019). First report of leaf spot and anthracnosis caused by Pestalotiopsis spp. on strawberry in Puebla, México. Plant Dis., 103, 2668-2671 https://doi.org/10.1094/PDIS-05-19-1010-PDN

Orga, P. J. (2021). Manejo agronómico del cultivo de arándano (Vaccinium corymbosum L.) en contenedores en Villacurí, Ica

https://hdl.handle.net/20.500.12996/4981

Proarándanos (2023). Asociación de productores de arándanos del Perú. Disponible en: http://Exportaciones, proarándanos (proarandanos.org) [accesado el 30 de mayo de 2023]

Rebellato-Urtizberea, M. J. (2011). Prospección de enfermedades en el cultivo de arándano (Vaccinium corymbosum L.) en la zona norte y sur del Uruguay y su relación con variables meteorológicas. https://hdl.handle.net/20.500.12008/9689

Rebollar, A., Minnis, A. M, Dixon, L. J., et al. (2011). First report of leaf rust of blueberry caused by Thekopsora minima in Mexico. Plant Dis, 95, 772-772 https://doi.org/10.1094/pdis-12-10-0885

Rodríguez-Gálvez, E., Hilário, S., Lopes, A., & Alves, A. (2020). Diversity and pathogenicity of Lasiodiplodia and Neopestalotiopsis species associated with stem blight and dieback of blueberry plants in Peru. European Journal of Plant Pathology, 157(1), 89-102. https://doi.org/10.1007/s10658-020-01983-1

Sathya, K., Parthasarathy, S., Thiribhuvanamala, G., & Prabakar, K. (2017). Morphological and molecular variability of Lasiodiplodia theobromae causing stem end rot of mango Tamil Nadu, India. International Journal of Pure & Applied Bioscience, 5(6), 1024-1031

Silva, S., Costa, E. M., Veiga, M., et al. (2020). Health promoting properties of blueberries: A review. Critical Reviews in Food Science and Nutrition, 60, 181-200. https://doi.org/10.1080/10408398.2018.1518895

Stefănescu, B., Călinoiu, L., Ranga, F., Fetea, F., Mocan, A., Vodnar, D., & Cri¸san, G. (2020). The chemical and biological profiles of leaves from commercial blueberry varieties. Plants, 9(1193).1-19. https://doi.org/10.3390/plants9091193

Steyaert, R. L. (1955). Pestalotia, Pestalotiopsis et Truncatella. Bulletin du Jardin botanique de l'Etat, Bruxelles/Bulletin van den Rijksplantentuin. Brussel, 191-199. https://doi.org/10.2307/3667065

Sutton, B. (1980). The Coelomycetes: Fungi imperfect with Pycnidia, Acervuli and Stromata. Commonwealth Mycological Institute Kew. Surrey. England.

Walters, D. (2009.) Disease control in crops. Biological and environmentally friendly approaches. Singapore, Blackwell. 263 p. http://hdl.handle.net/123456789/123

Wang, P., Hur, M., Cai, Y., Takeda, F., DeVetter, L., & Chen, J. (2023). Microbial Load of Fresh Blueberries Harvested by Different Methods. Foods, 12(1047), 1-10. https://doi.org/10.3390/foods12051047

Yao, X., Guo, H., Zhang, K., Zhao, M., Ruan, J., & Chen, J. (2023). Trichoderma and its role in biological control of plant fungal and nematode disease. Frontiers in microbiology, 14, 1160551. https://doi.org/10.3389/fmicb.2023.1160551

Zhang, J., Nie, J. Y., Li, J., et al. (2020). Evaluation of sugar and organic acid composition and their levels in highbush blueberries from two regions of China. Journal of Integrative Agriculture, 19 (9), 2352-2361. https://doi.org/10.1016/S2095-3119(20)63236-1

Published

2024-01-01

How to Cite

Aguilar-Anccota, R. ., Imán-Castillo, M. Á. ., Silupú-Masías, J. A. ., Rafael-Rutte, R. ., Calle-Cheje, Y. H. ., Campos e Silva, I. A. ., Morales-Pizarro, A. ., Maldonado Duque, E. ., & Javier Alva, J. . (2024). Prospecting and management alternatives of fungal diseases associated in the blueberry crop (Vaccinium corymbosum L.). Agroindustrial Science, 13(3), 163-171. https://doi.org/10.17268/agroind.sci.2023.03.07

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Artículos de investigación