Impact of leachate on soil microbial diversity and its treatment

Authors

DOI:

https://doi.org/10.17268/sci.agropecu.2024.023

Keywords:

Landfill leachate, temporary landfill, soil microbial diversity, soil quality parameters, coagulation - flocculation, water treatment

Abstract

This study analyzed the impact of leachate from a temporary landfill on soil microbial diversity in Tingo María, Huánuco region, Peru. Three treatments were used: untreated soil (S), addition of stream water (T0), leachate (T1), and leachate treated by coagulation and flocculation (T2), with 828.5 ml/week added in three weekly doses. Soil samples were collected from the Reserved Forest of the Universidad Nacional Agraria de la Selva. Twenty-one randomly distributed soil samples were taken and homogenized for analysis. Soil quality parameters measured included sand, clay, silt, texture, pH, organic matter, nitrogen, phosphorus and potassium. As for microorganisms, viable aerobes, lactobacilli, actinomycetes, fungi, nitrogen-fixing bacteria, and Escherichia coli were quantified using specific culture and counting methods for each of them. To evaluate the impact of the leachate on microbial diversity, equity indices (Shannon and inverse Simpson), dominance indices (complementary Simpson and Berger Parker) and the percentage composition of each microorganism per treatment were used. An ANOVA was performed to estimate differences in microbial diversity, with a Tukey test at a significance level of α = 0.05. The study showed that leachates affect soil microbial diversity, reducing equity and increasing the dominance of certain species such as E. coli. They also alter physicochemical parameters, decreasing organic matter and nitrogen but increasing other elements such as phosphorus and potassium. This could have implications for soil health and functionality.

References

Amokrane, A., Comel, C., & Veron, J. (1997). Landfill leachates pretreatment by coagulation-flocculation. Water Research, 31(11), 2775–2782. https://doi.org/10.1016/S0043-1354(97)00147-4

Bünemann, E. K., Bongiorno, G., Bai, Z., Creamer, R. E., De Deyn, G., et al. (2018). Soil quality – A critical review. Soil Biology and Biochemistry, 120, 105–125. https://doi.org/10.1016/J.SOILBIO.2018.01.030

Dagwar, P. P., & Dutta, D. (2024). Landfill leachate a potential challenge towards sustainable environmental management. Science of The Total Environment, 926, 171668. https://doi.org/10.1016/J.SCITOTENV.2024.171668

Djeffal, K., Bouranene, S., Fievet, P., Déon, S., & Gheid, A. (2019). Treatment of controlled discharge leachate by coagulation-flocculation: influence of operational conditions. Separation Science and Technology, 56(1), 168–183. https://doi.org/10.1080/01496395.2019.1708114

Fida, M., Li, P., Alam, S. M. K., Wang, Y., Nsabimana, A., & Shrestha, P. S. (2024). Review of Groundwater Nitrate Pollution from Municipal Landfill Leachates: Implications for Environmental and Human Health and Leachate Treatment Technologies. Exposure and Health, 2024, 1–25. https://doi.org/10.1007/S12403-023-00624-2

Gaudie Ley, M. B. R., Cardoso Junior, R. A. F., de Mendonça, H. V., Nascentes, A. L., & Batista da Silva, L. D. (2021). Comparison between prediction models and monitored data on leachate generation from a sanitary landfill in the metropolitan region of Rio de Janeiro, Brazil. International Journal of Hydrology, 5(2), 58–64. https://doi.org/10.15406/ijh.2021.05.00266

Giller, K. E., Witter, E., & Mcgrath, S. P. (1998). Toxicity of heavy metals to microorganisms and microbial processes in agricultural soils: a review. Soil Biology and Biochemistry, 30(10–11), 1389–1414. https://doi.org/10.1016/S0038-0717(97)00270-8

Gu, Z., Feng, K., Li, Y., & Li, Q. (2022). Microbial characteristics of the leachate contaminated soil of an informal landfill site. Chemosphere, 287, 132155. https://doi.org/10.1016/J.CHEMOSPHERE.2021.132155

Jones, R. T., Robeson, M. S., Lauber, C. L., Hamady, M., Knight, R., & Fierer, N. (2009). A comprehensive survey of soil acidobacterial diversity using pyrosequencing and clone library analyses. The ISME Journal 2009 3:4, 3(4), 442–453. https://doi.org/10.1038/ismej.2008.127

Leff, J. W., Jones, S. E., Prober, S. M., Barberán, A., Borer, E. T., Firn, J. L., Harpole, W. S., Hobbie, S. E., Hofmockel, K. S., Knops, J. M. H., McCulley, R. L., La Pierre, K., Risch, A. C., Seabloom, E. W., Schütz, M., Steenbock, C., Stevens, C. J., & Fierer, N. (2015). Consistent responses of soil microbial communities to elevated nutrient inputs in grasslands across the globe. Proceedings of the National Academy of Sciences of the United States of America, 112(35), 10967–10972. https://doi.org/10.1073/PNAS.1508382112

López-Vega, M. E., & Santos-Herrero, R. (2017). La recirculación de lixiviados de rellenos sanitarios en biodigestores a escala de laboratorio. Tecnología Química, XXXVII(3), 470-483.

Manrique De Lara, L. (2018). Relación entre los parámetros meteorológicos durante el periodo 1947-2016 con el comportamiento climático en Tingo Maria. Tesis doctoral, Universidad Nacional Hermilio Valdizan. Perú.

Maurya, S., Abraham, J. S., Somasundaram, S., Toteja, R., Gupta, R., & Makhija, S. (2020). Indicators for assessment of soil quality: a mini-review. Environmental Monitoring and Assessment 2020 192:9, 192(9), 1–22. https://doi.org/10.1007/S10661-020-08556-Z

Pozo Bejarano, J., García Gutierrez, J. A., & Vásquez Pérez, Y. (2020). Estimación del caudal medio de lixiviados generados en el vertedero de Viñales, Pinar del Río. Avances, 22(3), 1–11.

Salehi, N., Azhdarpoor, A., & Shirdarreh, M. (2020). The effect of different levels of leachate on phytoremediation of pyrene-contaminated soil and simultaneous extraction of lead and cadmium. Chemosphere, 246, 125845. https://doi.org/10.1016/J.CHEMOSPHERE.2020.125845

Semrau, J. D. (2011). Current knowledge of microbial community structures in landfills and its cover soils. Applied Microbiology and Biotechnology, 89(4), 961–969. https://doi.org/10.1007/S00253-010-3024-2

Shukla, S. K., Sharma, L., Jaiswal, V. P., Pathak, A. D., Tiwari, R., Awasthi, S. K., & Gaur, A. (2020). Soil quality parameters vis-a-vis growth and yield attributes of sugarcane as influenced by integration of microbial consortium with NPK fertilizers. Scientific Reports 2020 10:1, 10(1), 1–17. https://doi.org/10.1038/s41598-020-75829-5

Sinton, L. W., Finlay, R. K., & Hannah, D. J. (2010). Distinguishing human from animal faecal contamination in water: A review. New Zealand Journal of Marine and Freshwater Research, 32(2), 323–348. https://doi.org/10.1080/00288330.1998.9516828

Sinton, L. W., Hall, C. H., Lynch, P. A., & Davies-Colley, R. J. (2002). Sunlight inactivation of fecal indicator bacteria and bacteriophages from waste stabilization pond effluent in fresh and saline waters. Applied and Environmental Microbiology, 68(3), 1122–1131. https://doi.org/10.1128/AEM.68.3.1122-1131.2002

Smith, V. H. (2003). Eutrophication of freshwater and coastal marine ecosystems: A global problem. Environmental Science and Pollution Research, 10(2), 126–139. https://doi.org/10.1065/ESPR2002.12.142

Song, U., & Lee, E. J. (2010). Environmental and economical assessment of sewage sludge compost application on soil and plants in a landfill. Resources, Conservation and Recycling, 54(12), 1109–1116. https://doi.org/10.1016/J.RESCONREC.2010.03.005

Tchobanoglous, G., Theisen, H., & Vigil, S. A. (1993). Integrated solid waste management: engineering principles and management issues. New York, McGraw-Hill.

Wang, J., Ji, H., Wang, S., Liu, H., Zhang, W., Zhang, D., & Wang, Y. (2018). Probiotic Lactobacillus plantarum promotes intestinal barrier function by strengthening the epithelium and modulating gut microbiota. Frontiers in Microbiology, 9(AUG), 383517. https://doi.org/10.3389/FMICB.2018.01953

Wydro, U., Wołejko, E., Sokołowska, G., Leszczyński, J., & Jabłońska-Trypuć, A. (2022). Investigating Landfill Leachate Influence on Soil Microbial Biodiversity and Its Cytotoxicity. Water, 14(22), 3634. https://doi.org/10.3390/w14223634

Yeilagi, S., Rezapour, S., & Asadzadeh, F. (2021). Degradation of soil quality by the waste leachate in a Mediterranean semi-arid ecosystem. Scientific Reports 2021 11:1, 11(1), 1–12. https://doi.org/10.1038/s41598-021-90699-1

Zahran, H. H. (1999). Rhizobium-Legume Symbiosis and Nitrogen Fixation under Severe Conditions and in an Arid Climate. Microbiology and Molecular Biology Reviews, 63(4), 968–989. https://doi.org/10.1128/MMBR.63.4.968-989.1999

Zhou, W., Chai, J., Xu, Z., Qin, Y., Cao, J., & Zhang, P. (2024). A review of existing methods for predicting leachate production from municipal solid waste landfills. Environmental Science and Pollution Research, 31(11), 16131–16149. https://doi.org/10.1007/S11356-024-32289-Y

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Published

2024-05-13

How to Cite

Puerta-Tuesta, R. H., Aguirre, C. ., Guerra Lu, J. K., Cerna-Cueva, A. F., Rios-Garcia, W., Dueñas-Tuesta, M., & Paredes, C. (2024). Impact of leachate on soil microbial diversity and its treatment. Scientia Agropecuaria, 15(2), 301-310. https://doi.org/10.17268/sci.agropecu.2024.023

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