Characterization of Fusarium species that affect the asparagus crops cultivation in Veracruz, Mexico
DOI:
https://doi.org/10.17268/sci.agropecu.2022.031Keywords:
Fusariosis, Identification, Pathogenicity, Shoot, Molecular characterization, Morphometric characterizationAbstract
Asparagus cultivation is highly profitable and is appreciated in international gourmet cuisine. In Mexico, the production of this vegetable is mainly for export, in the state of Veracruz it has been recently introduced. The objective of the research was to characterize by morphology, molecular techniques and pathogenicity test the Fusarium species isolated from symptomatic tissues of asparagus shoot. A directed sampling was carried out on plants that showed rot, wilting and dark brown spots. Fungal isolates were obtained from diseased shoots, which were disinfected and seeded on potato dextrose agar. Isolates were purified in monosporic cultures by hyphal tip. Four species of Fusarium were identified by taxonomic morphology, in a selective carnation leaf agar medium, then pathogenicity tests were carried out, complying with Koch's postulates. Next, the strains that were pathogenic were characterized by molecular techniques, and their identity as Fusarium oxysporum and Fusarium proliferatum was confirmed. The information obtained allows the implementation of appropriate management strategies for pathogenic species and thereby avoids economic losses in the short, medium, and long term in the asparagus crop.
References
Arbeláez, G. (2000). Algunos aspectos de los hongos del género Fusarium y de la especie Fusarium oxysporum. Agronomía Colombiana, 17, 11-22.
Arie, T. (2019). Fusarium diseases of cultivated plants, control, diagnosis, and molecular and genetic studies. Journal of Pest Science, 44(4), 275-281.
Borrego-Benjumea, A., Basallote-Ureba, M.J., Melero-Vara, J.M. & Abbasi, P.A. (2014). Characterization of Fusarium isolates from asparagus fields in southwestern Ontario and influence of soil organic amendments on Fusarium crown and root rot. Phytopathology, 104(4), 403-415.
Cañedo, V. & Ames, T. (2004). Manual de laboratorio para el manejo de hongos entomopatógenos. Centro Internacional de la Papa (CIP), 62 p. Lima, Perú.
Corpas-Hervias, C., Melero-Vara, J. M., Molinero-Ruiz, M. L., Zurera-Muñoz, C. & Basallote-Ureba, M. J. (2006). Characterization of isolates of Fusarium spp. obtained from asparagus in Spain. Plant Disease, 90, 1441-1451.
Dietzel, K., Valle, D., Fierer, N., U´ren, J. M. & Barberán, A. (2019). Geographical distribution of fungal plant pathogens in dust across the United States. Frontiers in Ecology and Evolution, 7, 304.
Elmer, W. H. (2000). Incidence of infection of asparagus spears marketed in Connecticut by Fusarium spp. Plant Disease, 84, 831-834.
Elmer, W. D. & Pignatello, J. J. (2001). Effect of biochar amendments on mycorrhizal associations and Fusarium crown and root rot of asparagus in replant soils. Plant Disease, 95, 960-966.
Elmer, W. H. (2015). Management of Fusarium crown and root rot of asparagus. Crop Protection, 73, 2-6.
Farahani-Kofoet, R.D., Witzel, K., Graefe, J., Grosch, R. & Zrenner, R. (2020). Species-specific impact of Fusarium infection on the root and shoot characteristics of asparagus. Pathogens, 9, 509.
Hernández, M. A., Muiño, B. L., Roson, A. C. & Cazola, G. C. (2011). Hongos y oomycetes fitopatógenos en viveros de piña Ananas comosus (L.) merril en Ciego de Ávila, Cuba. Fitosanidad, 15(3), 137-142.
Hu, W., Zhang, X., Godana, E.A., Gu, X., Zhao, L. & Zhang H. (2021). Yarrowia lipolytica reduces the disease incidence of asparagus infected by Fusarium proliferatum by affecting respiratory metabolism and energy status. Biological control, 159, 104625.
Hunjan, M.S. & Lore, J.S. (2020). Climate change: Impact on plant pathogens, diseases, and their management. In K. Jabran, S. Florentine and B.S. Chauhan, eds. Crop protection under changing climate, Springer International Publishing, 85-100 p.
Kato-Noguchi, H., Nakamura, K., Ohno, O., Suenaga, K. & Okuda, N. (2017). Asparagus decline: Autotoxicity and autotoxic compounds in asparagus rhizomes. Journal of Plant Physiology, 213, 23-29.
Leslie, J. F., Summerell, B. A. & Bullock, S. (2006). The Fusarium laboratory manual. Blackwell Publishing Professional, Ames, Iowa, USA.
Martínez, F. E., Martínez, J. P., Guillen, S. D., Peña, C. G. & Hernández-Hernández, V. (2015). Diversidad de Fusarium en las raíces de caña de azúcar (Saccharum officinarum) en el estado de Morelos, México. Revista Mexicana de Micología, 42, 33-43.
Mašková, L., Simmons, R. W., Deeks, L. K. & Baets, S. D. (2021). Best management practices to alleviate deep-seated compaction in asparagus (Asparagus officinalis) interrows (UK). Soil and Tillage Research, 213, 105124.
Möller, E.M., Bahnweg, G., Sandermann & H. Geiger, H. (1992). A simple and efficient protocol for isolation of high molecular weight DNA from filamentous fungi, fruit bodies and infected plant tissues. Nucleic Acids Research, 20(22), 6115-6116.
Parikh, L., Kodati, S., Eskelson, M. J., & Adesemoye, A. O. (2018). Identification and pathogenicity of Fusarium spp. in row crops in Nebraska. Crop Protection, 108, 120-127.
Quilambaqui, J. & Miguel, A. (2005). Aislamiento e identificación de especies de Fusarium spp. asociadas al declinamiento del esparrago (Asparagus officinalis L.) en cinco municipios de Guanajuato, México. Revista Tecnológica ESPOL, 18(1), 135-140.
Quilambaqui, J. M., Zavaleta, M. E. & Mora, A. G. (2004). Patogenicidad de tres especies de Fusarium asociadas con el declinamiento de espárrago (Asparagus officinalis L.) en Guanajuato. Revista Mexicana de Fitopatología, 22(1), 30-34.
Ristaino, J. B., Anderson, P. K., Bebber, D. P., Brauman, K. A., Cunniffe, N. J., et al. (2021). The persistent threat of emerging plant disease pandemics to global food security. Proceedings of the National Academy of Sciences of the United States of America, 118(23), e2022239118.
Schreuder, W., Lamprecht, S. C., Marasas, W. F. O. & Calitz, F. J. (1995). Pathogenicity of three Fusarium species associated with asparagus decline in South Africa. Plant Disease, 79(2), 177-181.
SIAP. Servicio de Información Agroalimentaria y Pesquera (2021). Anuario Estadístico de la Producción Agrícola https://nube.siap.gob.mx/cierreagricola/
Singha, I. M., Kakoty, Y., Unni, B. G., Das, J. & Kalita, M. C. (2016). Identification and characterization of Fusarium sp. using ITS and RAPD causing fusarium wilt of tomato isolated from Assam, North East India. Journal of Genetic Engineering & Biotechnology, 14(1), 99-105.
Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013). MEGA6: Molecular evolutionary genetics analysis Version 6.0. Molecular Biology and Evolution, 30(12), 2725-2729.
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