Causal agent of anthracnose in the pea crop (Pisum sativum L.) in northern of Perú: Symptomatology, isolation and identification, pathogenicity and control
DOI:
https://doi.org/10.17268/sci.agropecu.2021.001Keywords:
Pisum sativum, anthracnose, Colletotrichum spp., fungicides, pathogenicityAbstract
Pea (Pisum sativum L.) is an economically important crop and of great demand in the national and international market, due to the considerable number of families that depend on its cultivation, especially in the northern and central highlands of Peru. However, the anthracnose is one of the limiting factors for low productive and causes economic losses. The aim of this study was to describe the symptoms, culturally and morphometrically identify the causal agent and to evaluate under in vitro the efficacy of fungicides. Isolation was made from samples of leaves and pods with anthracnose symptoms collected in the crop field. Healthy pea was used in the pathogenicity test, and discs of PDA medium containing isolated fungi were inoculated into them. The poisoned food technique was used, and colony growths were measured to evaluate the effects of fungicides. The results based on the symptoms, morphological and cultural characteristics described, the isolates were identified as Colletotrichum lindemuthianum, C. truncatum y Ascochyta pisi, which was confirmed with Koch's postulates. Trifloxystrobin+tebuconazole was found to be the most effective fungicide followed by tebuconazole, procloraz and mancozeb completely inhibited mycelial growth (100%) of identified fungi. These results are useful for the control of Colletotrichum lindemuthianum, C. truncatum y Ascochyta pisi in the pea crop.
References
Agrios, G. (1995). Fitopatología. S.A. de C. V. México, Mexico: Limusa.
Almaraz, S. A., Ayala, E. V., Landero, V. N., Tlatilpa, S. I., & Nieto, A. D. (2019). First Report of Colletotrichum truncatum of Solanum lycopersicum in Mexico. Plant Disease, 103(7), 1782-1782.
Armesto, C., Maia, F. G. M., Monteiro, F. P., & Abreu, M. S. (2019). Exoenzymes as a pathogenicity factor for Colletotrichum gloeosporioides associated with coffee plants. Summa Phytopathologica, 45(4), 368-373.
Armstrong-Cho, C. L., & Banniza, S. (2006). Glomerella truncata sp. nov., the teleomorph of Colletotrichum truncatum. Mycol Res, 110, 951-956.
Auyong, A. S., Ford, R., & Taylor, P. W. (2015). The role of cutinase and its impact on pathogenicity of Colletotrichum truncatum. Journal Plant Pathology & Microbiology, 6(3), 259.
Bellé, C., Ramos, R. F., Moccellin, R., & Jacobsen de Farias, C. R. (2020). Detección de Colletotrichum coccodes causantes de antracnosis foliar en Pisum sativum en el sur de Brasil. Journal of Plant Pathol, 102, 255.
Boersma, S. J., Depuydt, D. J., Vyn, R. J., Gillard, Ch. L. (2020). Fungicide efficacy for control of anthracnose of dry bean in Ontario. Crop protection, 127.
Camarena, F., Huaringa, A., & Osorio, U. (2014). Innovación fitotecnia del haba (Vicia faba L.), arveja (Pisum sativum L.) y lenteja (Lens culinaris Medik). (1a ed.). Lima, Perú: Universidad Nacional Agraria La Molina.
Carmona, M. & Sautua, F. (2017). La problemática de la resistencia de hongos a fungicidas. causas y efectos en cultivos extensivos. Agronomía & Ambiente, 37(1), 1-19.
Cnossen-Fassoni, A., Bazzolli, D. M., Brommonschenkel, S. H., Fernandes de Araújo, E., & Vieira de Queiroz, M. (2013). The pectate lyase encoded by the pecCl1 gene is an important determinant for the aggressiveness of Colletotrichum lindemuthianum. J Microbiol, 51, 461-470.
Conner, R. L., Gillard, Ch. L. Mcrae, K. B., Hwang, S. F., Chen, Y.Y., Hou, A., Penner, W. G., & Turnbull, G. D. (2019). Survival of the bean anthracnose fungus (Colletotrichum lindemuthianum) on crop debris in Canada. Can J Plant Path, 41(2), 209-217.
De-Silva, D. D., Ades, P., Crous, P. W., & Taylor, P. W. J. (2017). Colletotrichum species associated with chili anthracnose in Australia. Plant Pathol, 66, 254-267.
Dias, M. D., Pinheiro, V. F., & Café-Filho, A. C. (2016). Impact of anthracnose on the yield of soybean subjected to chemical control in the north region of Brazil. Summa Phytopathol, 42, 18-23.
French, E. R. & Hebert, T. T. (1980). Métodos de investigación fitopatológica. San José, Costa Rica: IICA.
He, Y., Chen, Q., Shu, C., Mei Yang, M., & Zhou, E. (2016). Colletotrichum truncatum, a new cause of anthracnose on Chinese flowering cabbage (Brassica parachinensis) in China. Trop Plant Pathol, 4, 183-192.
Jevtic, R., Nagl, N., Lalošević, M., Taški-Ajduković, K., Stojšin, V., & May, Ch. L. (2019). First report of ascochyta blight on field pea (Pisum sativum) caused by Didymella pisi in Bosnia and Herzegovina. Plant Disease, American Phytopathological Society, 103(6), 1427-1428.
Kitchen, J. L., Van den Bosch, F., Paveley, N. D., Helps, J., & Van den Berg, F. (2016). The evolution of fungicide resistance resulting from combinations of foliar-acting systemic seed treatments and foliar-applied fungicides: a modeling analysis. PLoS ONE, 11, e0161887.
Landero-Valenzuela, N., Lara-Viveros, F. M., Andrade-Hoyos, P., Aguilar-Pérez, L. A., & Aguado-Rodríguez, G. J. (2016). Alternatives for the control of Colletotrichum spp. Revista Mexicana de Ciencias Agrícolas, 7(5), 1189-1198.
Latorre, G. (1989). Fungicidas y nematicidas. Avances y aplicabilidad. Santiago de Chile, Chile: Facultad de Agronomía de la Pontificia Universidad de Chile.
Liu, N., Xu, S., Yao, X., Zhang, G., Mao, W., Hu, Q., Feng, Z., & Gong, Y. (2016). Studies on the control of Ascochyta blight in field peas (Pisum sativum L.) caused by Ascochyta pinodes in Zhejiang Province, China. Front Microbiol, 7, 481.
Maiza, B., Siles, M., Ríos, R. & Gabriel, J. (2015). Comportamiento de catorce líneas mejoradas de arveja (Pisum sativum L.) en la zona de Challapata, Oruro. Journal of the Selva Andina Research Society, 6(1), 10-22.
McCreary, C. M., Depuydt, D., Vyn, R. J., & Gillard, C. L. (2016). Fungicide efficacy of dry bean white mold Sclerotinia sclerotiorum (Lib.) de Bary, and economic analysis at moderate to high disease pressure. Crop Protect, 82, 75-81.
Ministerio de Agricultura y Riego-MINAGRI. (2019). Encuesta nacional de intenciones de siembra 2019. Lima, Perú: Dirección general de seguimiento y evaluación de políticas dirección de estadística agraria.
Moges, A. D., Admassu, B., Belew, D., Yesuf, M., Njuguna, J., Kyalo, M., & Sita, R. Ghimire, S. R. (2016). Development of microsatellite markers and analysis of genetic diversity and population structure of Colletotrichum gloeosporioides from Ethiopia. PLoS One 11(3): e0151257.
Mont, R. M. (2002). Manejo integrado de enfermedades de las plantas. Lima, Peru: Ministerio de Agricultura, Servicio Nacional de Sanidad Agraria.
Nakamura, M., & Iwai, H. 2019. Functions and mechanisms: polygalacturonases from plant pathogenic fungi as pathogenicity and virulence factors. J Gen Plant Pathol, 85, 243-250.
Owati, A., Agindotan, B., & Burrows, M. (2019). First microsatellite markers developed and applied for the genetic diversity study and population structure of Didymella pisi associated with ascochyta blight of dry pea in Montana. Fungal Biology, 123, 384-392.
Padder, B. A., Kapoor, V., Kaushal, R. P. & Sharma, P. N. (2012). Identification and genetic diversity analysis of ascochyta species associated with blight complex of pea in a northwestern hill state of India. Agric Res, 1(4), 325-337.
Padder, B. A., Sharma, P. N., Awale, H. E., Kelly, J. D. (2017). Colletotrichum lindemuthianum, the causal agent of bean anthracnose. Journal of Plant Pathology, 99(2), 317-330.
Riccioni, L., Orzali, L., Romani, M., Annicchiarico, P., & Pecetti, L. (2019). Organic seed treatments with essential oils to control ascochyta blight in pea. Eur J Plant Pathol, 155, 831-840.
Rodríguez-Guerra, R., Ramírez-Rueda, M. T., Cabral-Enciso, M. García-Serrano, M., Lira-Maldonado, Z., Guevara-González, R. G., González-Chavira, M., & Simpson, J. (2005). Heterothallic mating observed between Mexican isolates of Glomerella lindemuthiana. Mycologia, 97(4), 793-803.
Rodríguez-Ortega, D., Vega-Jiménez, L., Murillo-Ilbay, Á., Peralta-Idrovo, E., & Rosas-Sotomayor, J. C. (2018). Variabilidad patogénica de Colletotrichum lindemuthianum y resistencia en germoplasma de Phaseolus vulgaris L. de Ecuador. Agronomía Mesoamericana, 29(1), 19-28.
Rogério, F., Gladieux, P., Massola, N. S., & Ciampi-Guillardi, M. (2019). Multiple introductions without admixture of Colletotrichum truncatum associated with soybean anthracnose in Brazil. Phytopathology, 109, 681-689.
Rojo-Báez, I., García-Estrada, R. S., León-Félix, J. Sañudo-Barajas, A., & Allende-Molar, R. (2016). Histopatología del proceso de infección de Colletotrichum truncatum en hojas de papaya y chícharo. Revista Mexicana de Fitopatología, 34(3), 316-325.
Rojo-Báez, I., García-Estrada, R. S., Sañudo-Barajas, A. J., León-Félix, J., & Allende-Molar, R. (2017a). Proceso de infección de Antracnosis por Colletotrichum truncatum en papaya Maradol. Rev Bras Frutic, 39, 1-5.
Rojo-Báez, I., Álvarez-Rodríguez, B., García-Estrada, R. S., León-Félix, J., Sañudo-Barajas, A., & Allende-Molar, R. (2017b). Current status of Colletotrichum spp. in Mexico: Taxonomy, characterization, pathogenesis and control. Revista Mexicana de Fitopatología, 35(3), 549-570.
Rubiales, D., Araújo, S. S., Vaz Patto, M. C., Rispail, N., & Valdés-López, O. (2018). Editorial: Advances in Legume Research. Forntiers in Plant Science, 9, 501.
Shafique, S., Shafique, S., & Ahmed, A. (2017). Defense response of Eucalyptus camaldulensis against black spot pathogen of Pisum sativum. South African Journal of Botany, 113, 428-436.
Sewedy, M. E., Atia, M. M., Zayed, M. A., & Ghonim, M. (2019). Molecular detection and controlling of seed-borne Colletotrichum spp. in common bean and soybean. Zagazig J Agric Res, 46, 6A.
Sivachandra-Kumar, N. T., & Banniza, S. (2017). Assessment of the effect of seed infection with Ascochyta pisi on pea in western Canada. Front Plant Sci, 8, 933.
Sutton, B. C. (1980). The Coelomycetes: Fungi Imperfecti with Pycnidia, Acervuli, and Stromata. Surrey, UK: Commonwealth Mycological Institute – Kew.
Tadja, A. 2018. Morphological and genotypic characterization of fungi associated with the Ascochyta blight complex in western regions of Algeria. European Scientific Journal, 14 (9), 276-284.
Taylor, J. W., Branco, S., Gao, Ch., Hann-Soden, Ch., Montoya, L., Sylvain, I., & Gladieux, P. (2017). Sources of fungal genetic variation and associating it with phenotypic diversity. Microbiol Spectr, 5(5): 1-21.
Torres‐Calzada, C., Tapia‐Tussell, R., Higuera‐Ciapara, I., Huchin‐Poot, E., Martín‐ Mex, R., Nexticapan‐Garcez, A., & Pérez‐Brito, D. (2018). Characterization of Colletotrichum truncatum from papaya, pepper and physic nut based on phylogeny, morphology and pathogenicity. Plant Pathol, 67, 821-830.
Valladolid-Chiroque, A. R. (2016). Leguminosas de grano cultivares y clases comerciales del Perú. Lima, Perú: Ministerio de Agricultura y Riego.
Watson, Ch. A., Reckling, M., Preissel, S., Bachinger, J., Bergkvist, G., Kuhlman, T., Lindstrom, K., Nemecek, T., Topp, C. F. E., Vanhatalo, A., Zander, P., Murphy-Bokern, D., & Stoddard, F. L. (2017). Grain legume production and use in European agricultural systems. Adv Agron, 144, 235-303.
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