Isolation of wild fermentative yeast strains from native fruits of Misiones province, Argentina

Autores/as

DOI:

https://doi.org/10.17268/agroind.sci.2025.03.14

Palabras clave:

wild yeasts, fruits, fermentation, osmotolerance, ethanol tolerance

Resumen

The objective of the present study was to isolate and select wild fermentative yeast strains to be used in the production of regional alcohol beverages. Yeasts were isolated from the spontaneous fermentation of must from different fruits of Misiones province and their ability to produce ethanol, as well as their tolerance to stress factors, were evaluated. Among a total of 62 isolated yeast strains, P2, P6, P7, and P11 (isolated from pitanga), and M31 (isolated from black mulberry) were preselected for presenting greater fermentation capacity. Strains P6 and P11 proved to be the most resistant to ethanol and more osmotolerant, could grow in the presence of SO2 and presented a good ethanol yield, which was 69.6% (P6) and 77% (P11) with respect to the maximum theoretical yield. The other yeast strains showed lower tolerance to the stress factors studied. According to the results of the present study, strains P6 and P11 were selected for further research focused on the production of regional fruit wines.

Citas

Ajit, E. J., Dominic, D., Farook, F., Promod, A., Kumar, B., Blesy, V. J., Sabu, K. R., Rajesh, B. R. & Pratap C. R. (2018). Preparation of wine from fruits of Musa accuminata and Ananas comosus; its physico-chemical analyses and sensory evaluation. Integrative food, nutrition and metabolism, 5(6), 1-5. https://doi.org/10.15761/IFNM.1000232

Alezandro, M. R., Dubé, P., Desjardins, Y., Lajolo, F. M., & Genovese, M. I. (2013). Comparative study of chemical and phenolic compositions of two species of jaboticaba: Myrciaria jaboticaba and Myrciaria cauliflora. Food Research International, 54(1), 468-477. https://doi.org/10.1016/j.foodres.2013.07.018

Campos-Rodriguez, J., Acosta-Coral, K., Moreno-Rojo, C., & Paucar-Menacho, L. M. (2023). Maracuyá (Passiflora edulis): Compo-sición nutricional, compuestos bioactivos, aprovechamiento de subproductos, biocontrol y fertilización orgánica en el cultivo. Scientia Agropecuaria, 14(4), 479-497, 2023. https://doi.org/10.17268/sci.agropecu.2023.040

Canché-Collí, C., Barahona, F., Medina-Medina, L. A., & Canto, A. (2021). The effect of sugar concentration on growth of yeast associated to floral nectar and honey. Scientia fungorum, 52, e1288, 1-11. https://doi.org/10.33885/sf.2021.52.1288

Chacón-Ordóñez, T., & Esquivel-Rodríguez, P. (2013). Frutos tropicales como fuente de carotenoides: biosíntesis, composición, biodisponibilidad y efectos del procesamiento. Revista Venezolana de Ciencia y Tecnología de Alimentos, 4(1), 1-23.

Girardelo, J. R., Munari, E. L., Dallorsoleta, J. C., Cechinel, G., Goetten, A. L., Sales, L. R., Reginatto, H., Chaves, V. C., Smaniotto, F. A., Somacal, S., Emanuelli, T., Benech, J. C., Soldi, C., Winter, E., & Conterato, G. M. (2020). Bioactive compounds, antioxidant capacity and antitumoral activity of ethanolic extracts from fruits and seeds of Eugenia involucrata DC. Food Research International, 137, 109615. https://doi.org/10.1016/j.foodres.2020.109615

Guevara-Bravo, C., Acevedo, J., & Peláez Jaramillo, C. (2014). Aislamiento y caracterización de una levadura floculante para producir etanol del banano de rechazo. Biotecnología en el Sector Agropecuario y Agroindustrial, 12(2), 151-159.

Gundogdu M., Muradoglu, F., Gazioglu Sensoy, R. I., & Yilmaz, H. (2011). Determination of fruit chemical properties of Morus nigra L., Morus alba L. and Morus rubra L. by HPLC. Scientia Horticulturae, 132, 37-41. https://doi.org/10.1016/j.scienta.2011.09.035

Haro-Altamirano, J. P., Zambrano-Cárdenas, G. O., Cazorla-Vinueza, X. R., Soplín, H., & Garzón, R. (2021). Evaluation of the sustainability of family farming farms, of two eco-types of Yellow Pitahaya (Selenicereus megalanthus), and their by-product. Polo del Conocimiento, 6(12), 301-319.

Koyuncu, F., Çetinbaş, M., & Ibrahim, M. (2014). Nutritional constituents of wild-grown black mulberry (Morus nigra L.). Journal of Applied Botany and Food Quality, 87, 93-96. https://doi.org/10.5073/JABFQ.2014.087.014

López-Álvarez, J. A. (2007). Selección y manipulación genética de una levadura tolerante y sobreproductora de etanol, Tesis de Maestría, Universidad Michoacana de San Nicolás de Hidalgo, México.

Lorenzini, M., Simonato, B., Slaghenaufi, D., Ugliano, M., & Zapparoli, G. (2019). Assessment of yeasts for apple juice fermentation and production of cider volatile compounds. LWT - Food Science and Technology, 99, 224-230. https://doi.org/10.1016/j.lwt.2018.09.075

Loyo-Trujillo, N. A., Vivar-Vera, G., Sánchez-Bazán, I., Jiménez-Guzmán, J., & Del Ángel-Zumaya, J. A. (2020). Caracterización y elaboración de una bebida fermentada tipo vino de ciruela roja var. Black Amber. Coloquio de investigación multidisciplinaria, 8(1), 1842- 1848. http://dx.doi.org/10.23857/dc.v7i1.1691

Maidana, S. A., Butiuk, A. P., Zubreski, E. R., Hours, R. A., Brumovsky, L. A., & Martos, M. A. (2019). Production of an endopolygalacturonase from Wickerhanomyces anomalus with disintegration activity on plant tissues. Biocatalysis and Agricultural Biotechnology, 18, 101042. https://doi.org/10.1016/j.bcab.2019.101042

Malacrino, P., Tosi, E., Caramia, G., Prisco, R., & Zapparoli, G. (2005). The vinification of partially dried grapes: A comparative fermentation study of Saccharomyces cerevisiae strains under high sugar stress. Letters in Applied Microbiology, 40, 466-472. https://doi.org/10.1111/j.1472-765X.2005.01713.x

Martos, M. A., Zubreski, E. R., Combina, M., Garro, O. A., & Hours, R. A. (2013). Isolation of a yeast strain able to produce a polygalacturonase with maceration activity of cassava roots. Food Science and Technology, 33(2), 332–338. http://dx.doi.org/10.1590/S0101-20612013005000047

Mercado, L., Dalcero, A., Masuelli, R., & Combina, M. (2007). Diversity of Saccharomyces strains on grapes and winery surfaces: analysis of their contribution to fermentative flora of Malbec wine from Mendoza (Argentina) during two consecutive years. Food Microbiology, 24(4), 403-412. https://doi.org/10.1016/j.fm.2006.06.005

Miranda-Castilleja, D. E., Ortiz-Barrera, E., Arvizu-Medrano, S. M., Ramiro-Pacheco, J., Aldrete-Tápia, J. A., & Martínez-Peniche, R. A. (2015). Aislamiento, selección e identificación de levaduras Saccharomyces spp. nativas de viñedos en Querétaro, México. Agrociencia, 49(7), 759-773.

Ortiz-Zamora, O., Cortés-García, R., Ramírez-Lepe, M., Gómez-Rodríguez, J., & Aguilar-Uscanga, M. G. (2009). Isolation and selection of ethanol-resistant and osmotolerant yeasts from regional agricultural sources in México. Journal of Food Process Engineering, 32, 775-86. https://doi.org/10.1111/j.1745-4530.2008.00244.x

Peña, C., & Arango, R. (2009). Evaluación de la producción de etanol utilizando cepas recombinantes de Saccharomyces cerevisiae a partir de melaza de caña de azúcar. Dyna, 76(159), 153-161.

Rompato, K., Franco, R. R., Somoza, S. N., & Rompato, L. S. (2015). Composición nutricional de frutos de Syagrus romanzoffiana (pindó) nativos de Formosa- Argentina. Boletim do Centro de Pesquisa e Processamento de Alimentos, 33(2), 105-112. http://dx.doi.org/10.5380/cep.v33i2.47296

Sahana, G. R., Balasubramanian, B., Joseph K. S., Pappuswamy, M., Liu, W. C., Meyyazhagan, A., Kamyab, H., Chelliapan, S., & Joseph, B. V. (2024). A review on ethanol tolerance mechanisms in yeast: current knowledge in biotechnological applications and future directions. Process Biochemistry, 138, 1-13. https://doi.org/10.1016/j.procbio.2023.12.024

Santamaría, P., López, R., López, E., Garijo, P., & Gutiérrez, A. (2008). Permanence of yeast inoculates in the winery ecosystem and presence in spontaneous fermentations. European Food Research and Technology, 227(5), 1563-1567. https://doi.org/10.1007/s00217-008-0855-5

Suárez-Valles, B., Pando-Bedriñana, R., Fernández-Tascón, N., González-Garcia, A., & Rodríguez-Madrera, R. (2005). Analytical differentiation of cider inoculated with yeast (Saccharomyces cerevisiae) isolated from Asturian (Spain) apple juice. LWT - Food Science and Technology, 38(5), 455-461. https://doi.org/10.1016/j.lwt.2004.07.008

Voget, C., Broncompagno, N., Villa Monte, I., Romero, M., Velarde. I., Filleira, G. S., & Borrajo, A. (2013). Vinos de fruta en América. La Alimentación Latinoamericana, 306, 56-67.

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Publicado

2025-10-10

Cómo citar

Maidana, S. A., Butiuk, A. P., Godoy, A. E., Martos, M. A., & Zubreski, E. R. (2025). Isolation of wild fermentative yeast strains from native fruits of Misiones province, Argentina. Agroindustrial Science, 14(3), 331-338. https://doi.org/10.17268/agroind.sci.2025.03.14

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Artículos de investigación