Caracterización composicional y estructural de once tipos de biomasa lignocelulósica y su potencial aplicación en la obtención de nanopolisacáridos y producción de polihidroxialcanoatos

D.  Haro; S.  Marquina-Barrios; A. Fuentes-Olivera; A. Quezada; J. Cruz-Monzón; L. Cueva-Almendras; Cindy Morán-González; Yulissa Ventura-Avalos; Juan Rojas-Fermín; G. Barraza-Jáuregui

doi:10.17268/sci.agropecu.2024.038

Authors

D. Haro Grupo de Investigación en Biopolímeros, Nanomateriales y Tecnología (GIBINTEC), Facultad de Ciencias Agropecuarias, Universidad Nacional de Trujillo, La Libertad, Perú. https://orcid.org/0000-0001-6295-7578
S. Marquina-Barrios Grupo de Investigación en Biopolímeros, Nanomateriales y Tecnología (GIBINTEC), Facultad de Ciencias Agropecuarias, Universidad Nacional de Trujillo, La Libertad, Perú. https://orcid.org/0000-0002-8643-7195
A. Fuentes-Olivera Grupo de Ingeniería de Procesos y Biomateriales, Facultad de Ingeniería Química, Universidad Nacional de Trujillo, La Libertad, Perú. https://orcid.org/0000-0003-1311-3912
A. Quezada Grupo de Ingeniería de Procesos y Biomateriales, Facultad de Ingeniería Química, Universidad Nacional de Trujillo, La Libertad, Perú. https://orcid.org/0000-0002-0215-5175
J. Cruz-Monzón Grupo de Ingeniería de Procesos y Biomateriales, Facultad de Ingeniería Química, Universidad Nacional de Trujillo, La Libertad, Perú. https://orcid.org/0000-0001-9146-7615
L. Cueva-Almendras Centro de Innovación Productiva y Transferencia Tecnológica Agroindustrial Chavimochic, Instituto Tecnológico de la Producción, La Libertad, Perú. https://orcid.org/0000-0003-2608-1374
Cindy Morán-González Centro de Innovación Productiva y Transferencia Tecnológica Agroindustrial Chavimochic, Instituto Tecnológico de la Producción, La Libertad, Perú. https://orcid.org/0000-0003-1363-9922
Yulissa Ventura-Avalos Grupo de Investigación en Biopolímeros, Nanomateriales y Tecnología (GIBINTEC), Facultad de Ciencias Agropecuarias, Universidad Nacional de Trujillo, La Libertad, Perú. https://orcid.org/0009-0009-7239-433X
Juan Rojas-Fermín Grupo de Investigación en Biopolímeros, Nanomateriales y Tecnología (GIBINTEC), Facultad de Ciencias Agropecuarias, Universidad Nacional de Trujillo, La Libertad, Perú. https://orcid.org/0009-0004-9082-5379
G. Barraza-Jáuregui Grupo de Investigación en Biopolímeros, Nanomateriales y Tecnología (GIBINTEC), Facultad de Ciencias Agropecuarias, Universidad Nacional de Trujillo, La Libertad, Perú. https://orcid.org/0000-0002-0376-2751

DOI:

https://doi.org/10.17268/sci.agropecu.2024.038

Keywords:

circular economy, biorefinery, agricultural waste, agro-industrial waste, structural carbohydrates, high-performance liquid chromatography, biopolymers

Abstract

The valorization and use of lignocellulosic biomass from food processing to obtain value-added products is crucial to improve sustainability and reduce waste management costs. This potential for cost reduction is a reason for optimism, as it allows agro-industrial waste to be transformed into valuable resources, contributing to the circular economy. This study focused on the compositional and structural characterization of eleven types of lignocellulosic biomass (LCB) to evaluate their potential in producing nanopolysaccharides and polyhydroxyalkanoates. Parameters such as humidity, ash, proteins, extractives, structural carbohydrates, and lignin were analyzed in passion fruit peels, artichoke bracts, asparagus peels, lemon peels, orange peels, avocado seed, potato peels, cassava peels, sugar cane bagasse, rice husk, and rice straw. The results showed that fruit peels and other waste had a high extractive content (28.05%), while the content of lignin and structural carbohydrates varied between 69.66% and 30.53% and 22.2% and 8.84%, respectively. In addition to the characterization, this BLC's potential for producing biopolymers was explored, highlighting its relevance in various industries such as food and materials engineering. These findings underline the importance of using local natural resources sustainably, opening new opportunities to develop innovative applications such as pickering emulsions, biodegradable packaging, aerogels, hydrogels, and functional foods. These applications represent promising areas for future research and technological development.

References

Ahmad, A. N. S., Sofian-Seng, N.-S., Othaman, R., Abdul, H., Mohd, N. S., Lim, S. J., & Wan, W. A. (2023). A Review on Agro-industrial Waste as Cellulose and Nanocellulose Source and Their Potentials in Food Applications. Food Reviews International, 39(2), 663-688. https://doi.org/10.1080/87559129.2021.1926478

Alcaide, I. V., Hamdi, A., Guilleín-Bejarano, R., Jiménez-Araujo, A., & Rodríguez-Arcos, R. (2023). Sustainable valorization of co-products from asparagus cultivation by obtaining bioactive compounds. Frontiers in plant science, 14, 1199436.

Aoudi, B., Boluk, Y., & Gamal El-Din, M. (2022). Recent advances and future perspective on nanocellulose-based materials in diverse water treatment applications. Science of The Total Environment, 843, 156903. https://doi.org/10.1016/j.scitotenv.2022.156903

Ayala, J. R., Montero, G., Coronado, M. A., García, C., Curiel-Alvarez, M. A., León, J. A., Sagaste, C. A., & Montes, D. G. (2021). Characterization of orange peel waste and valorization to obtain reducing sugars. Molecules, 26(5), 1348.

Baite, T. N., Purkait, M. K., & Mandal, B. (2023). Synthesis of lignin from waste leaves and its potential application for bread packaging: A waste valorization approach. International Journal of Biological Macromolecules, 235, 123880. https://doi.org/10.1016/j.ijbiomac.2023.123880

Barja, F. (2021). Bacterial nanocellulose production and biomedical applications. Journal of Biomedical Research, 35(4), 310.

Bilal, M., Qamar, S. A., Qamar, M., Yadav, V., Taherzadeh, M. J., Lam, S. S., & Iqbal, H. M. N. (2024). Bioprospecting lignin biomass into environmentally friendly polymers—Applied perspective to reconcile sustainable circular bioeconomy. Biomass Conversion and Biorefinery, 14(4), 4457-4483. https://doi.org/10.1007/s13399-022-02600-3

Cazón, P., & Vázquez, M. (2021). Bacterial cellulose as a biodegradable food packaging material: A review. Food Hydrocolloids, 113, 106530.

Chieng, B. W., Lee, S. H., Ibrahim, N. A., Then, Y. Y., & Loo, Y. Y. (2017). Isolation and characterization of cellulose nanocrystals from oil palm mesocarp fiber. Polymers, 9(8), 355.

Daoud, L., & Ali, M. B. (2020). Halophilic microorganisms: Interesting group of extremophiles with important applications in biotechnology and environment. En Physiological and biotechnological aspects of extremophiles (pp. 51-64). Elsevier. https://www.sciencedirect.com/science/article/pii/B9780128183229000058

De Souza, L., Manasa, Y., & Shivakumar, S. (2020). Bioconversion of lignocellulosic substrates for the production of polyhydroxyalkanoates. Biocatalysis and Agricultural Biotechnology, 28, 101754.

Dórame-Miranda, R. F., Gámez-Meza, N., Medina-Juárez, L. Á., Ezquerra-Brauer, J. M., Ovando-Martínez, M., & Lizardi-Mendoza, J. (2019). Bacterial cellulose production by Gluconacetobacter entanii using pecan nutshell as carbon source and its chemical functionalization. Carbohydrate Polymers, 207, 91-99. https://doi.org/10.1016/j.carbpol.2018.11.067

Goñi, I., García-Alonso, A., Alba, C., Rodríguez, J. M., Sánchez-Mata, M. C., Guillén-Bejarano, R., & Redondo-Cuenca, A. (2024). Composition and Functional Properties of the Edible Spear and By-Products from Asparagus officinalis L. and Their Potential Prebiotic Effect. Foods, 13(8), 1154.

Goodman, B. A. (2020). Utilization of waste straw and husks from rice production: A review. Journal of Bioresources and Bioproducts, 5(3), 143-162.

Gorgieva, S., Jančič, U., Cepec, E., & Trček, J. (2023). Production efficiency and properties of bacterial cellulose membranes in a novel grape pomace hydrolysate by Komagataeibacter melomenusus AV436T and Komagataeibacter xylinus LMG 1518. International journal of biological macromolecules, 244, 125368.

Hames, B., Sluiter, A., & Scarlata, C. (2008). Determination of Protein Content in Biomass: Laboratory Analytical Procedure (LAP): Issue Date, 07/17/2005. National Renewable Energy Laboratory.

Hasanin, M. S., Abdelraof, M., Hashem, A. H., & El Saied, H. (2023). Sustainable bacterial cellulose production by Achromobacter using mango peel waste. Microbial Cell Factories, 22(1), 24. https://doi.org/10.1186/s12934-023-02031-3

Hermiati, E., Wijaya, H., & Pramasari, D. A. (2024). Extraction, Isolation, Purification, and Potential Application of Xylose and Xylooligosaccharides from Lignocellulosic Biomass. En M. A. R. Lubis, S. H. Lee, E. Mardawati, S. Rahimah, P. Antov, R. Andoyo, Ľ. Krišťák, & B. Nurhadi (Eds.), Biomass Conversion and Sustainable Biorefinery (pp. 229-267). Springer Nature Singapore. https://doi.org/10.1007/978-981-99-7769-7_11

Iglesias-Montes, M. L., Soccio, M., Siracusa, V., Gazzano, M., Lotti, N., Cyras, V. P., & Manfredi, L. B. (2022). Chitin nanocomposite based on plasticized poly (lactic acid)/poly (3-hydroxybutyrate)(PLA/PHB) blends as fully biodegradable packaging materials. Polymers, 14(15), 3177.

Inayati, I., Puspita, R. I., & Fajrin, V. L. (2018). Extraction of pectin from passion fruit rind (Passiflora edulis var. Flavicarpa Degener) for edible coating. AIP conference proceedings, 1931(1). https://pubs.aip.org/aip/acp/article-abstract/1931/1/030002/831308

John, I., Muthukumar, K., & Arunagiri, A. (2017). A review on the potential of citrus waste for D -Limonene, pectin, and bioethanol production. International Journal of Green Energy, 14(7), 599-612. https://doi.org/10.1080/15435075.2017.1307753

Kassambara, A., & Mundt, F. (2020). Factoextra: Extract and Visualize the Results of Multivariate Data Analyses. R package version 1.0.7.999, https://github.com/kassambara/factoextra

Koller, M. (2017). Advances in polyhydroxyalkanoate (PHA) production. Bioengineering, 4(4), 88.

Kumari, R., Sakhrie, M., Kumar, M., Vivekanand, V., & Pareek, N. (2023). Enhanced production of bacterial cellulose employing banana peel as a cost-effective nutrient resource. Brazilian Journal of Microbiology, 54(4), 2745-2753. https://doi.org/10.1007/s42770-023-01151-7

Li, W., Cicek, N., Levin, D. B., Logsetty, S., & Liu, S. (2020). Bacteria-triggered release of a potent biocide from core-shell polyhydroxyalkanoate (PHA)-based nanofibers for wound dressing applications. Journal of Biomaterials Science, Polymer Edition, 31(3), 394-406. https://doi.org/10.1080/09205063.2019.1693882

Liang, S., & McDonald, A. G. (2015). Anaerobic digestion of pre-fermented potato peel wastes for methane production. Waste management, 46, 197-200.

Lo, J. S. C., Chen, X., Chen, S., Miao, Y., Daoud, W. A., Tso, C. Y., Firdous, I., Deka, B. J., & Lin, C. S. K. (2024). Fabrication of biodegradable PLA-PHBV medical textiles via electrospinning for healthcare apparel and personal protective equipment. Sustainable Chemistry and Pharmacy, 39, 101536.

Lopresto, C. G., Meluso, A., Di Sanzo, G., & Calabrò, V. (2018). Lemonene Recovery from Waste Lemon Peels with Supercritical Extraction. En A. Kallel, M. Ksibi, H. Ben Dhia, & N. Khélifi (Eds.), Recent Advances in Environmental Science from the Euro-Mediterranean and Surrounding Regions (pp. 1147-1149). Springer International Publishing. https://doi.org/10.1007/978-3-319-70548-4_331

MacLellan, J., Chen, R., Yue, Z., Kraemer, R., Liu, Y., & Liao, W. (2017). Effects of protein and lignin on cellulose and xylan anaylses of lignocellulosic biomass. Journal of Integrative Agriculture, 16(6), 1268-1275.

Mahato, R. P., Kumar, S., & Singh, P. (2023). Production of polyhydroxyalkanoates from renewable resources: A review on prospects, challenges and applications. Archives of Microbiology, 205(5), 172. https://doi.org/10.1007/s00203-023-03499-8

Mamma, D., & Christakopoulos, P. (2014). Biotransformation of Citrus By-Products into Value Added Products. Waste and Biomass Valorization, 5(4), 529-549. https://doi.org/10.1007/s12649-013-9250-y

Marx, S., & Radebe, L. J. (2018). Microwave-assisted recovery of monomeric sugars from an acidic steam treated wood hydrolysate. Heliyon, 4(11). https://www.cell.com/heliyon/fulltext/S2405-8440(18)33731-9

Mhgub, I. M., Hefnawy, H. T., Gomaa, A. M., & Badr, H. A. (2018). Chemical composition, antioxidant activity and structure of pectin and extracts from lemon and orange peels. Zagazig Journal of Agricultural Research, 45(4), 1395-1404.

Mujtaba, M., Fernandes Fraceto, L., Fazeli, M., Mukherjee, S., Savassa, S. M., Araujo De Medeiros, G., Do Espírito Santo Pereira, A., Mancini, S. D., Lipponen, J., & Vilaplana, F. (2023). Lignocellulosic biomass from agricultural waste to the circular economy: A review with focus on biofuels, biocomposites and bioplastics. Journal of Cleaner Production, 402, 136815. https://doi.org/10.1016/j.jclepro.2023.136815

Narisetty, V., Cox, R., Bommareddy, R., Agrawal, D., Ahmad, E., Pant, K. K., Chandel, A. K., Bhatia, S. K., Kumar, D., & Binod, P. (2022). Valorisation of xylose to renewable fuels and chemicals, an essential step in augmenting the commercial viability of lignocellulosic biorefineries. Sustainable Energy & Fuels, 6(1), 29-65.

Nascimento, E. S., Barros, M. O., Cerqueira, M. A., Lima, H. L., de Fatima Borges, M., Pastrana, L. M., Gama, F. M., Rosa, M. F., Azeredo, H. M., & Gonçalves, C. (2021). All-cellulose nanocomposite films based on bacterial cellulose nanofibrils and nanocrystals. Food Packaging and Shelf Life, 29, 100715.

Núñez-Gómez, V., San Mateo, M., González-Barrio, R., & Periago, M. J. (2024). Chemical Composition, Functional and Antioxidant Properties of Dietary Fibre Extracted from Lemon Peel after Enzymatic Treatment. Molecules, 29(1), 269.

Ogrizek, L., Lamovšek, J., Čuš, F., Leskovšek, M., & Gorjanc, M. (2021). Properties of bacterial cellulose produced using white and red grape bagasse as a nutrient source. Processes, 9(7), 1088.

Oliver-Ortega, H., Evon, P., Espinach, F. X., Raynaud, C., & Méndez, J. A. (2024). Polyhydroxy-3-Butyrate (PHB) Composite Materials Reinforced with Barley Waste Straw Fibres for Agriculture Applications: Production, Characterization and Scale-Up Analysis. Materials, 17(8), 1901. https://doi.org/10.3390/ma17081901

Ona, J. I., Halling, P. J., & Ballesteros, M. (2019). Enzyme hydrolysis of cassava peels: Treatment by amylolytic and cellulolytic enzymes. Biocatalysis and Biotransformation, 37(2), 77-85. https://doi.org/10.1080/10242422.2018.1551376

Órbenes, G., Rodríguez-Seoane, P., Torres, M. D., Chamy, R., Zúñiga, M. E., & Domínguez, H. (2021). Valorization of artichoke industrial by-products using green extraction technologies: Formulation of hydrogels in combination with paulownia extracts. Molecules, 26(14), 4386.

Ortiz-Sanchez, M., Solarte-Toro, J. C., Orrego-Alzate, C. E., Acosta-Medina, C. D., & Cardona-Alzate, C. A. (2021). Integral use of orange peel waste through the biorefinery concept: An experimental, technical, energy, and economic assessment. Biomass Conversion and Biorefinery, 11(2), 645-659. https://doi.org/10.1007/s13399-020-00627-y

Pecha, M. B., & Garcia-Perez, M. (2020). Chapter 29-pyrolysis of lignocellulosic biomass: Oil, char, and gas, Editor (s): Anju Dahiya, bioenergy. Academic Press.

Pereira, B., Marcondes, W. F., Carvalho, W., & Arantes, V. (2021). High yield biorefinery products from sugarcane bagasse: Prebiotic xylooligosaccharides, cellulosic ethanol, cellulose nanofibrils and lignin nanoparticles. Bioresource Technology, 342, 125970.

Phonphuak, N., & Chindaprasirt, P. (2015). Types of waste, properties, and durability of pore-forming waste-based fired masonry bricks. Eco-efficient masonry bricks and blocks, 103-127.

Phothong, N., Boontip, T., Chouwatat, P., Aht-Ong, D., & Napathorn, S. C. (2024). Preparation and characterization of astaxanthin-loaded biodegradable polyhydroxybutyrate (PHB) microbeads for personal care and cosmetic applications. International Journal of Biological Macromolecules, 257, 128709.

Priyadarshi, R., Ghosh, T., Purohit, S. D., Prasannavenkadesan, V., & Rhim, J.-W. (2024). Lignin as a sustainable and functional material for active food packaging applications: A review. Journal of Cleaner Production, 143151.

Rahmani, Z., Khodaiyan, F., Kazemi, M., & Sharifan, A. (2020). Optimization of microwave-assisted extraction and structural characterization of pectin from sweet lemon peel. International Journal of Biological Macromolecules, 147, 1107-1115.

Reyes, M. M., Gómez-Sánchez, I., & Espinoza, C. M. (2017). Tablas peruanas de composición de alimentos. Instituto Nacional de Salud. https://repositorio.ins.gob.pe///handle/20.500.14196/1034

Ruiz-Aceituno, L., García-Sarrió, M. J., Alonso-Rodriguez, B., Ramos, L., & Sanz, M. L. (2016). Extraction of bioactive carbohydrates from artichoke (Cynara scolymus L.) external bracts using microwave assisted extraction and pressurized liquid extraction. Food chemistry, 196, 1156-1162.

Saba, B., Bharathidasan, A. K., Ezeji, T. C., & Cornish, K. (2023). Characterization and potential valorization of industrial food processing wastes. Science of The Total Environment, 868, 161550. https://doi.org/10.1016/j.scitotenv.2023.161550

Sampaolesi, S., Briand, L. E., Saparrat, M. C. N., & Toledo, M. V. (2023). Potentials of Biomass Waste Valorization: Case of South America. Sustainability, 15(10), Article 10. https://doi.org/10.3390/su15108343

Sandoval-Contreras, T., González Chávez, F., Poonia, A., Iñiguez-Moreno, M., & Aguirre-Güitrón, L. (2023). Avocado Waste Biorefinery: Towards Sustainable Development. Recycling, 8(5), 81. https://doi.org/10.3390/recycling8050081

Senthilkumar, S. R., Ashokkumar, B., Raj, K. C., & Gunasekaran, P. (2005). Optimization of medium composition for alkali-stable xylanase production by Aspergillus fischeri Fxn 1 in solid-state fermentation using central composite rotary design. Bioresource Technology, 96(12), 1380-1386.

Sharma, V., Sehgal, R., & Gupta, R. (2021). Polyhydroxyalkanoate (PHA): Properties and modifications. Polymer, 212, 123161.

Sluiter, A., Hames, B., Hyman, D., Payne, C., Ruiz, R., Scarlata, C., Sluiter, J., Templeton, D., & Wolfe, J. (2008). Determination of total solids in biomass and total dissolved solids in liquid process samples: Laboratory Analytical Procedure (LAP). National Renewable Energy Laboratory, 2-5.

Sluiter, A., Hames, B., Ruiz, R., Scarlata, C., Sluiter, J., & Templeton, D. (2005). Determination of ash in biomass laboratory analytical procedure. National Renewable Energy Laboratory Analytical Procedure, Golden, CO, 19.

Sluiter, A., Hames, B., Ruiz, R., Scarlata, Ch., Sluiter, J., Templeton, D., & Crocker, D. (2008a). Determination of structural carbohydrates and lignin in biomass: Laboratory analytical procedure (LAP). In Technical Report NREL/TP-510-42618. National Renewable Energy Laboratory Golden, CO, USA., 1617(1), 1-16.

Sluiter, A., Ruiz, R., Scarlata, C., Sluiter, J., & Templeton, D. (2008b). Determination of Extractives in Biomass: Laboratory Analytical Procedure (LAP); Issue Date 7/17/2005. Technical Report.

Stoica, M. (2020). Biodegradable nanomaterials for drink packaging. En Nanotechnology in the Beverage Industry (pp. 609-632). Elsevier. https://www.sciencedirect.com/science/article/pii/B9780128199411000213

Tateishi, A., Shiba, H., Ogihara, J., Isobe, K., Nomura, K., Watanabe, K., & Inoue, H. (2007). Differential expression and ethylene regulation of β-galactosidase genes and isozymes isolated from avocado (Persea americana Mill.) fruit. Postharvest Biology and Technology, 45(1), 56-65.

Trache, D., Tarchoun, A. F., Derradji, M., Hamidon, T. S., Masruchin, N., Brosse, N., & Hussin, M. H. (2020). Nanocellulose: From Fundamentals to Advanced Applications. Frontiers in Chemistry, 8. https://doi.org/10.3389/fchem.2020.00392

Turan, O., Isci, A., Yılmaz, M. S., Tolun, A., & Sakiyan, O. (2024). Microwave-assisted extraction of pectin from orange peel using deep eutectic solvents. Sustainable Chemistry and Pharmacy, 37, 101352.

Ul-Islam, M., Alhajaim, W., Fatima, A., Yasir, S., Kamal, T., Abbas, Y., Khan, S., Khan, A. H., Manan, S., & Ullah, M. W. (2023). Development of low-cost bacterial cellulose-pomegranate peel extract-based antibacterial composite for potential biomedical applications. International Journal of Biological Macromolecules, 231, 123269.

Vaishnav, A., & Choudhary, D. K. (Eds.). (2021). Microbial Polymers: Applications and Ecological Perspectives. Springer Singapore. https://doi.org/10.1007/978-981-16-0045-6

Wang, W., Du, G., Li, C., Zhang, H., Long, Y., & Ni, Y. (2016). Preparation of cellulose nanocrystals from asparagus (Asparagus officinalis L.) and their applications to palm oil/water Pickering emulsion. Carbohydrate Polymers, 151, 1-8.

Wickham, H., et al. (2019). Welcome to the Tidyverse. J. Open Source Softw., 4, 1686. https://doi.org/10.21105/joss.01686

Xu, H., Sanchez-Salvador, J. L., Blanco, A., Balea, A., & Negro, C. (2023). Recycling of TEMPO-mediated oxidation medium and its effect on nanocellulose properties. Carbohydrate Polymers, 319, 121168.

Ye, X., Zhang, Y., Liu, T., Chen, Z., Chen, W., Wu, Z., Wang, Y., Li, J., Li, C., & Jiang, T. (2022). Beta-tricalcium phosphate enhanced mechanical and biological properties of 3D-printed polyhydroxyalkanoates scaffold for bone tissue engineering. International Journal of Biological Macromolecules, 209, 1553-1561.

Zhang, F., Shen, R., Li, N., Yang, X., & Lin, D. (2023). Nanocellulose: An amazing nanomaterial with diverse applications in food science. Carbohydrate Polymers, 304, 120497. https://doi.org/10.1016/j.carbpol.2022.120497