Protocol for ex vitro and in vitro micropropagation of Cucurbita moschata and C. ecuadorensis, native to Peru and Ecuador, of nutritional and medicinal importance
DOI:
https://doi.org/10.17268/sci.agropecu.2023.012Palavras-chave:
Endemic species, micropropagation, Peruvian species, semi-aseptic conditions, shoot elongationResumo
Cucurbitaceae species constitute a family with a wide worldwide distribution and great nutritional and medicinal importance. The Cucurbita genus is the most important of the family with valuable species such as C. maxima and C. moschata. However, the large-scale production of sex specific plants using the conventional propagation methods has various limitations. The tissue culture such as micropropagation can help overcome these limitations. The aim of this study was to establish a protocol for ex vitro and in vitro clonal propagation of two varieties of Cucurbita moschata: ‘loche’, a variety of commercial vegetative propagation and endemic in Lambayeque, and ‘chuyán’ another variety of very restricted cultivation in some Andean regions of the north of the Peru. Likewise, the in vitro propagation of ‘chisguín’ (C. ecuadorensis), a threatened endemic wild species from the coast of Ecuador. ‘Loche’ was propagated ex vitro by microcutting with a node and leaf, and micropropagated in MS culture medium supplemented with 0.02 mg L-1 IAA and 0.02 mg L-1 GA3. Seedlings of ‘chuyán’ and ‘chisguin’ were micropropagated in MS with with 0.02 mg L-1 IAA, 0.5 mg L-1 BAP and 0.02 mg L-1 GA3. This study constitutes the first report on micropropagation of Peruvian and Ecuadorian varieties and species of the Cucurbita genus.
Referências
Agogbua, J. U., & Okoli, B. E. (2016). In vitro shoot multiplication of Zehneria capillacea (Schumach) C. Jeffrey, a wild edible cucurbit in the Niger Delta region of Nigeria. Acta Horticulturae, 1113, 219-224.
Ananthakrishnan, G., Xia, X., Elman, C., Singer, S., Paris, H. S., Gal-On, A., & Gaba, V. (2003). Shoot production in squash (Cucurbita pepo) by in vitro organogenesis. Plant Cell Reports, 21(8), 739-746.
Arciniega-Carreón, I.-Y., Oliver-Salvador, C., Ramírez-Sotelo, M.-G., & Salas, C. (2017). Efficient in vitro plant regeneration from internode explants of Ibervillea sonorae: An antidiabetic medicinal plant. HortScience, 52(7), 1000-1005.
Aworunse, O., Omasoro, R., & Soneye, B. (2019). Effect of low BAP levels on multiple shoots induction in indigenous Nigerian pumpkin (Cucurbita pepo Linn.). Journal of Physics: Conference Series, 1299(17), 0121003.
Awotedu, B. F., Omolola, T. O., Akala, A. O., Awotedu, O. L., & Olaoti-Laaro, S. O. (2021). Vegetative propagation: A unique technique of improving plants growth. World News of Natural Sciences, 35, 83-101.
Bowes, B. G. (1990). A simple micropropagation techniques utilizing non-sterile explants and its potential in conservation. Professional Horticulture, 4, 113-120.
Brako, L., & Zarucchi, J. L. (1993). Catalogue of the flowering plants and gymnosperms of Peru. (Monographs in Systematic Botany Vol. 45.) Missouri Botanical Garden, St. Louis, MO. 1286 p.
Castillo, J., & Moncayo, S. (2019). Actividad biológica y composición química en extractos de Cucurbita ecuadorensis H. C. Cutler & Whitaker. Revista Científica Ciencias Naturales y Ambientales, 13(2), 92-104.
Chant, S. R., & Marden, J. A. (1958). A method for rapid propagation of cassava cuttings. Tropical Agriculture, 35, 195-199.
Choudhary, S. K., Patel, A. K., Harish, Shekhawat, S., & Shekhawat, N. (2017). An improved micropropagation system, ex vitro rooting and validation of genetic homogeneity in wild female Momordica dioica: an underutilized nutraceutical vegetable crop. Physiology and Molecular Biology of Plants, 23(3), 713-722.
Chuengpanya, R., Pornchuti, W., Muangkroot, A., Jenjittikul, T., & Chuenboonngarm, N. (2020). In vitro propagation of Zehneria platysperma (W.J. de Wilde & Duyfjes) H. Schaef. & S.S. Renner (Cucurbitaceae), an endemic plant of Thailand. Acta Horticulturae 1285, 221-230.
del Castillo, A., Molinares, P., Campo, M., & Bettin, A. (2017). Actividad antibacteriana del extracto total de hojas de Cucurbita moschata Duchesne (Ahuyama). Revista Cubana de Plantas Medicinales 22(1).
Delgado-Paredes, G. E., Kato, M. J., Vásquez-Dueñas N., Minchala-Patiño, J., & Rojas-Idrogo, C. (2012). Cultivo de tejidos de Piper sp. (Piperaceae): propagación, organogénesis y conservación de germoplasma in vitro. Revista Colombiana de Biotecnología, XIV(2), 49-60.
Delgado-Paredes, G. E., Vásquez-Díaz, C., Esquerre-Ibañez, B., Bazán-Sernaqué, P., & Rojas-Idrogo, C. (2021). In vitro tissue culture in plants propagation and germplasm conservation of economically important species in Peru. Scientia Agropecuaria, 12(3), 337-349.
Delgado-Paredes, G. E., Rojas-Idrogo, C., Sencie-Tarazona, A., & Vásquez-Núñez, L. (2014). Caracterización de frutos y semillas de algunas cucurbitáceas en el norte del Perú. Revista Fitotecnia Mexicana, 37(1), 7-20.
Dhumal, S. S., Naik, B. V., & Nimbalkar, M. S. (2020). Advances in tissue culture of cucurbits: A Review. International Journal of Current Microbiology and Applied Sciences, 9(8), 2887-2910.
Haque, M. E., Sarkar, M. A. R., Mahmud, M. A., & Rezwana, D. (2008). In vitro propagation of pumpkin and ash gourd through nodal segments. Journal of Bio-Science, 16, 67-71.
Hesari, Z., Sharifdini, M., Sharifi-Yazdi, M. K., Ghafari, S., Ghasemi, S., et al. (2020). In vitro effects of pumpkin (Cucurbita moschata) seed extracts on echinococcus granulosus protoscoleces. Iranian Journal of Parasitology, 15(1), 76-83.
Kabir, M. R., Akhond, M. A. Y., Al-Amin, M., & Shahidul, M. (2016). In vitro regeneration of sweetgourd (Cucurbita moschata Duch.). Plant Tissue Culture and Biotechnology, 26(1), 67-75.
Kim, K., Kim, C. K., & Han, J-S. (2010). In vitro regeneration from cotyledon explants in fig leaf gourd (Cucurbita ficifolia Bouche.) a rootstock for Cucurbitaceae. Plant Biotechnology Reports, 4(2), 101-107.
Kurtar, E. S., Balkaya, A., & Kandemir, D. (2016). Evaluation of haploidization efficiency in winter squash (Cucurbita maxima Duch.) and pumpkin (Cucurbita moschata Duch.) through anther culture. Plant Cell, Tissue and Organ Culture, 127(2), 497-511.
Lambertini, E., Penolazzi, L., Pellielo, G., Pipino, C., Pandolfi, A., et al. (2021). Pro-osteogenic properties of violina pumpkin (Cucurbita moschata) leaf extracts: Data from in vitro human primary cell culture. Nutrients, 13(8), 2633.
León-Yánez, S., Valencia, R., Pitmam, N., et al. (comp.). (2019). Libro Rojo de Plantas Endémicas del Ecuador. Publicaciones del Herbario QCA, Pontificia Universidad Católica del Ecuador, Quito. https://bioweb.bio/floraweb/librorojo
Malviya, R., & Sharma, A. (2021). Therapeutic Potential and Pharmaceutical Applications of Cucurbita. Current Nutrition & Food Science, 17(4), 363-373.
Miljić, M., Rocchetti, G., Krstić, S., Mišan, A., Brdar-Jokanović, M., et al. (2021). Comparative in vitro antioxidant capacity and terpenoid profiling of pumpkin fruit pulps from a Serbian Cucurbita maxima and Cucurbita moschata breeding collection. Antioxidants, 10(10), 1580.
Mukherjee, P. K., Singha, S., Kar, A., Chanda, J., Banerjee, S., et al. (2022). Therapeutic importance of Cucurbitaceae: A medicinally important family. Journal of Ethnopharmacology, 28210, 114599.
Neves, R. J., Souza, L. S., & de Oliveira, E. J. (2020). A leaf bud technique for rapid propagation of cassava (Manihot esculenta Crantz). Crop Science, Sci. agric. (Piracicaba, Braz.), 77(2). 2020.
Obembe, O. O., Aworunse, O. S., Bello, O. A., & Ani, A. (2017). Multiple shoots induction from indigenous Nigerian pumpkin (Cucurbita pepo L.). Annual Research and Review in Biology, 17(5), 1-10.
Paris, H. S., & Brown, R. N. (2005). The genus of pumpkin and squash. HortScience, 40(6), 1620-1630.
Pateña, L. F., & Barba, R. C. (1979). Rapid propagation of cassava by leaf-bud cuttings. Philippine Journal of Crop Science, 4(2-3), 53-62.
Ruiz-Carrera, V., & Sánchez, A. J. (2008). Development of a model for in vitro culture of Vallisneria americana Michx. Universidad y Ciencia, 24(3), 205-218.
Santiana, J., & Tye, A. (2017). Cucurbita ecuadorensis. En: León-Yánez, S., Valencia, R., Pitmam, N., Endara, L., Ulloa Ulloa, C., Navarrete, H. (comp.). Libro Rojo de Plantas Endémicas del Ecuador. Publicaciones del Herbario QCA, Pontificia Universidad Católica del Ecuador, Quito.
Sharma, U., Kataria, V., & Shekhawat, N. S. (2017). In vitro propagation, ex vitro rooting and leaf micromorphology of Bauhinia racemosa Lam.: a leguminous tree with medicinal values. Physiology and Molecular Biology of Plants, 23(4), 969-977.
Sidik, N. J., Hashim, S. N., Mohamad, Y. S., & Abdullah, S. (2012). Effects of natural and synthetic cytokinin hormone on shoot regeneration of rockmelon (Cucumis melo) Glamour cv. by using nodal explants. ISBEIA 2012 – IEEE Symposium on Business, Engineering and Industrial Applications, 719-723.
Stipp, L. C. L., Monteiro-Hara, A. C. B. A., & Mendes, B. M. J. (2012). In vitro organogenesis of zucchini squash cv. Caserta. Horticultura Brasileira, 30(2), 274-278.
Tariq, A., Afrasiab, H., Farhat, F. (2020). In vitro micropropagation of Citrullus colocynthis (L.) Schrad: An endangered medicinal plant. Advancements in Life Sciences 8(1), 52-56.
Teppner, H. (2004). Notes on Lagenaria and Cucurbita (Cucurbitaceae) – Review and new contributions. Phyton 44(2), 245-308.
Toro, J. C., Roca, W. M., & Cock, J. H. (1982). Métodos de multiplicación acelerada de material genético promisorio de yuca Manihot esculenta Crantz. En: Domínguez C. E. (comp.). Yuca: Investigación, Producción y Comercialización. PNUD, CIAT. Pp. 147-152.
Ugás, T. C., & Bustamante, F. (2006). Loche: a unique pre-Columbian squash locally grown in north coastal Peru. Cucurbitaceae 2006, Asheville, North Carolina, USA, 17.21 September, pp. 333-340 ref. 6.
Vemula, S., Koppula, T., Jogam, P., & Mohammed, M. (2020). In vitro high frequency multiplication and assessment of genetic fidelity of Corallocarpus epigeus: an endangered medicinal plant. Vegetos 33(1), 63-73.
Wang, P. F., Xin, X., Wei, H., Qiu, X., & Liu, B. (2020). In vitro regeneration, ex vitro rooting and foliar stoma studies of Pseudostellaria heterophylla (Miq.). Agronomy 2020, 10, 949.
Zhang, Y., Zhou, J., Wu, T., & Cao, J. (2008). Shoot regeneration and the relationship between organogenic capacity and endogenous hormonal contents in pumpkin. Plant Cell Tissue and Organ Culture 93(3), 323-331.
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