Inoculation of Burkholderia cepacia and Gluconacetobacter diazotrophicus on phenotype and biomass of Triticum aestivum var. Nana-F2007 at 50% of nitrogen fertilizer

Authors

  • Jesús Jaime Hernández-Escareño Universidad Autónoma de Nuevo León. Escobedo
  • Pedro Gabriel Morales Universidad Michoacana de San Nicolás de Hidalgo, Morelia
  • Rodolfo Farias Rodriguez Universidad Michoacana de San Nicolás de Hidalgo, Morelia
  • Juan Manuel Sánchez-Yáñez Universidad Michoacana de San Nicolás de Hidalgo, Morelia

DOI:

https://doi.org/10.17268/sci.agropecu.2015.01.01

Keywords:

xylem colonization, rhizosphere, synergism, radical uptake

Abstract

Wheat (Triticum aestivum L) consuming requires of nitrogen fertilizer (NF), as ammonium nitrate (NH4NO3), which one in excess causes lost soil productivity. An alternative to reduce and optimize NF to wheat is to inoculate with endophytic promoting growth bacteria (EPGB), as genus Burkholderia cepacia and Gluconacetobacter diazotrophicus able to improve radical uptake of NF, its suggesting by inducing synthesis of growth promoting vegetal substances (GPVS). The aim of this research was to evaluate the inoculation of Burkholderia cepacia and Gluconacetobacter diazotrophicus on phenology and biomass of T. aestivum at 50% dose of NF. A trial in greenhouse condition was conducted inoculating seed T. aestivum´s with both EPGB by measuring its phenology: (PH) plant height, (RL) root length and biomass: total fresh weight (TFW) and dry (TDW) at seedling and flowering stages. Results showed a positive effect of B. cepacia in wheat on its TDW with 0.61g value statistically significant compared to 0.53g TDW of wheat used as relative control fed with NF 100% dose (RC). B. cepacia and G. diazotrophicus inoculated to wheat had a positive increased on its TDW with 4.23 g value statistically significant compared to 1.13 g TDW of wheat used as RC. Conclusion suggested that B. cepacia and G. diazotrophicus by synthetized GPVS had a positive effect on wheat growth at reduced dose of NF.

References

Asghar, H.N.; Zahir, Z.A.; Arshad, M.; Khaliq, A. 2002. Relationship between in vitro production of auxins by rhizobacteria and their growth-promoting activities in Brassica juncea L.Biology Fertility Soils 35: 231-237.

Bashan, Y.; Levanony, H. 1990. Current status of Azospirillum inoculation technology: Azospirillum as a challenge for agriculture. Candian Journal Microbiology 36: 591-608.

Cárdenas-Navarro, R; Sánchez-Yáñez, J.M.; Farías-Rodriguez F.; Peña-Cabriales J.J. 2004. Los aportes del nitrógeno en la agricultura. Revista Chapingo Serie Horticultura 10: 173-178.

Calderón-Becerra, M.A.; Balandreau, J.; Sánchez-Yáñez, J.M. 2000. Bacterias fijadoras de nitrógeno asociada a las raíces de teocintle (Zea mays sp mexicana L) y maíz (Zea mays L). Cuatro Vientos 16: 4-12.

Castellanos-Morales, V.; Villegas J.; Cárdenas-Navarro, R.; Farías-Rodriguez, R.; Sánchez-Yáñez, J.M. 2005. Diversity of Burkholderia cepacia associated to teocinte. Phyton 54: 27-38.

Camelo, M.; Vera, S.P.; Bonilla, R.R. 2011. Mecanismos de acción de las rizobacterias promotoras del crecimiento vegetal. Corpoica 12: 159-166.

Contreras-López, E.; Jaimez-Ordaz, J.; Hernández-Madrigal, T.; Añorve-Morga, J.; Beltrán-Hernández, R. 2008. Composición química de cebadas cultivadas bajo diferentes condiciones de labranza en tres localidades del estado de Hidalgo, México. Bioagro 20: 201-208.

Cortés, J.M.; Ortiz, A. 2008. Recomendaciones de fertilización para el cultivo de trigo. V jornada de transferencia de tecnología del cultivo de trigo. Pp. 7-18. Disponible en: http://www.fps.org.mx/divulgacion/ attachments/article/1009/v-jornada-trigo.pdf

Chelius, M.K.; Triplett, E.W. 2000. Prokaryotic Nitrogen Fixation; Model system for the analysis of a biological process. Ed Horizont Scientific Press. Hwitts Lane WymondahmNR18. England. pp: 1-20.

Dibut, B.; Ortega, M; Martínez, R.; Fey, L.; Ríos, Yoania. 2005. Nuevos aislados de Gluconacetobacter diazotrophicus en cultivos de importancia económica para Cuba. Cultivos tropicales 26: 5-10.

Döbereiner, J.; Baldani V. L. D,; Olivares, F.; Reis V. M. 1993. Endophytic diazotrophs: the key to BNF in gramineous plants. Eds. NA Hegazi, M Fayez y Monib M. pp: 395-408. Soy. Univ. en El Cairo Press, El Cairo, Egipto.

García, J.A.; Castillo, A.; Ramírez, M. E.; Rendón, G.; Larqué, M. U. 2001. Comparación de los procedimientos de Tukey, Duncan, Dunnett, HSU y Bechhofer para selección de medias. Agrociencia 35: 79-86.

García-Reyna, M.J.; Plata, G.D.; Cárdenas, N.R.; Farías, R.R.; Sánchez-Yáñez, J.M. 2001. Isolation and effect of inoculation of maize with endophytic bacteria of teocintle. Meeting III on Rhizosphere, Dijon, France.

García-Reyna, M.J.; Caballero, M.J.; Cárdenas, N.R.; Farías, R.R; Sánchez-Yáñez, J.M. 2000. Aislamiento y densidad de bacterias endófitas de teocintle. XXV. Congreso Nacional de Microbiología, Mérida, Yucatán, México.

García-González, M.M.; Farías-Rodríguez, R.; Peña-Cabriales, J.J.; Sánchez-Yáñez, J.M. 2005. Inoculación del trigo var. Pavón con Azospirillum spp y Azotobacter beijerinckii. Terra Latinoamericana 23: 65-72.

James, E.K. 2000. Nitrogen fixation in endophytic and associative symbiosis. Field Crops Research 65: 197-209.

Khalid, A.; Arshad, M.; Zahir, Z.A.; Khalid M. 2001. Relative efficiency of rhizobacteria for auxin biosynthesis. Journal of Biological Sciences 1: 750-754.

Mora, E.; Toro, M. 2007. Estimulación del crecimiento vegetal por Burkholderia cepacia, una cepa nativa de suelos ácidos de sabanas venezolanas. Agronomía Tropical 57: 123-128.

Plana, R.; Medina, N.; Moreno, I.; Ramírez, A. 1999. Efecto agronómico de la biofertilizacion con dos rizobacterias en la producción de trigo (Triticum aestivum L.) en cuba. Cultivos tropicales 20: 5-8.

Riggs, P.J.; Chelius, M.K.; Iniguez, A.L.; Kaeppler, S.M.; Triplett, E.W. 2001. Enhanced maize productivity by inoculation with diazotrophic bacteria. Australian Journal of Plant Physiology 28: 829-836.

Rosas, S.; Avanzini , G.; Carlier , E.; Pasluosta, C.; Pastor, N.; Rovera, M. 2009. Root colonization and growth promotion of wheat and maize by Pseudomonas aurantiaca SR1. Soil Biology and Biochemistry 41: 1802-1806.

Salazar, E.Z.; Nieves, B.; Ruíz, J.; Vila, J. 2008. Utilidad del Sistema API 20NE para identificar especies del género Acinetobacter y otros bacilos Gram negativos no fermentadores. Sociedad Venezolana de Microbiología 28: 89-95.

Sánchez-Yáñez J.M. 2007. Breve Tratado de Microbiología Agrícola, teoría y práctica, Ed. Instituto de Investigaciones Químico Biológicas. Universidad Michoacana de San Nicolás de Hidalgo. COSUSTENTA, SA de CV, CIDEM, SEDAGRO. Morelia, Michoacán, México.

Sevilla, M.; Burris, R.H.; Nirmala-Gunalapa, G.L.; Kennedy, C.; Gunalapa, N. 2001. Comparison of benedit to sugarcane plant growth and N15 incorporation following inoculation of sterile plants with Acetobacter diazotrophicus wild-type and Nif-mutant strains. Molecular Plant-Microbe Interactions 14: 358-366.

Tran Van, V.; Berge, O.; Ngo Ke, S.; Balandreau, J.; Heulin, T. 2000. Repeated benefical effects of rice inoculation with a strain of Burkholderia vietnamiensis on early and late yield components in low fertility sulphate acid of Vietnam. Plant and Soil 218: 273-284.

Tsavkelova, E.; Klimova, S.; Cherdyntseva, T.; Netrusov, A. 2006. Microbial producers of plant growth stimulators and their practical use: a review. Applied Biochemestry Microbiology 42: 117-126.

Villaseñor, M.H.; Espitia, R.E.; Huerta, E.J., Solas, M.E.; Ireta, M.J.; Osorio, A.L.; Perez, H.P. 2014. Nana F2007, cultivar de trigo para siembras de temporal en México. Revista Mexicana de Ciencias Agrícolas 7: 1363-1368.

Whitmore, A.P. 2000. The biological management of soil fertility project. Netherland Journal Agriculture Science 48: 115-122.

Youssef, H.; Fayez, M.; Monib, M.; Hegazi, N. 2004. Gluconacetobacter diazotrophicus: a natural endophytic diazotroph of Nile Delta sugarcane capable of establishing an endophytic association with wheat. Biology and Fertility of Soils 39: 391-397.

Received: 24/10/14

Accepted: 08/02/15

Corresponding author: E-mail: syanez@umich.mx (J.M. Sánchez-Yáñez).

Published

2015-03-31

How to Cite

Hernández-Escareño, J. J., Gabriel Morales, P., Farias Rodriguez, R., & Sánchez-Yáñez, J. M. (2015). Inoculation of Burkholderia cepacia and Gluconacetobacter diazotrophicus on phenotype and biomass of Triticum aestivum var. Nana-F2007 at 50% of nitrogen fertilizer. Scientia Agropecuaria, 6(1), 7-16. https://doi.org/10.17268/sci.agropecu.2015.01.01

Issue

Section

Original Articles

Most read articles by the same author(s)

Similar Articles

You may also start an advanced similarity search for this article.