A computational analysis revealed BES1 transcription factor and β-amylase as crosstalk elements in Upland cotton species (Gossypium sp.)

doi:10.17268/sci.agropecu.2024.033

Authors

Facultad de Ciencias Pecuarias y Biológicas. Universidad Técnica Estatal de Quevedo. Quevedo, Los Ríos https://orcid.org/0000-0003-2511-1789
Facultad de Ciencias Pecuarias y Biológicas. Universidad Técnica Estatal de Quevedo. Quevedo, Los Ríos https://orcid.org/0000-0001-9299-170X
Instituto Superior Tecnológico “Ciudad De Valencia”. Puebloviejo, Los Ríos https://orcid.org/0009-0002-5620-7628
Facultad de Ciencias Pecuarias y Biológicas. Universidad Técnica Estatal de Quevedo. Quevedo, Los Ríos https://orcid.org/0000-0003-1662-7337
CIRAD, UMR AGAP Institut, INRAE, Institut Agro, Univ Montpellier, Montpellier https://orcid.org/0000-0001-6321-4741
Université d´Avignon et des Pays du Vaucluse https://orcid.org/0000-0003-2852-5063

DOI:

https://doi.org/10.17268/sci.agropecu.2024.033

Keywords:

BAM, bioinformatics, BZR1, crosstalk, gene expression, transcription factor

Abstract

Cotton is a resilient and multipurpose crop, meeting major of the world’s textile needs while also yielding byproducts like edible oil and animal feed. Starch plays a crucial role in cotton fabric production. It enhances fabric strength by forming a protective film around cotton fibers, making them more resistant to wear and tear. BES1 (brassinosteroid insensitive 1) is a key regulator in brassinosteroid signaling. It controls thousands of target genes involved in development processes. Interestingly, two β-amylase proteins (BAM7 and BAM8) are part of the BES1 family, despite their primary function as β-amylases. β-Amylase (BAM) and BES1 are two gene families with functional and regulatory roles in controlling shoot growth and development by mediating brassinosteroid effects. They share similar domains and participate in various biological processes, tolerance and responses to stresses like salt and drought. In a computational analysis comparing Arabidopsis and Gossypium species, BAM and BES1 were characterized. BES1 genes were grouped into four clusters based on the comparison of the two species. Two clusters corresponded to BAM7 and BAM8, while the other two clusters were associated with BES1. The conserved nucleotide domain sequence is GCTGGATGG. Short tandem repeats include TG and TTG, which can serve as molecular markers. BES1 is specifically linked to cellulose and fiber production and holds promise as a candidate for plant selection and breeding in Gossypium (cotton).

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