Chickpea (Cicer arietinum L.) responds to inoculation with Azotobacter vineladii and Burkholderia cepacia at reduced dose of nitrogen fertilizer
DOI:
https://doi.org/10.17268/sci.agropecu.2014.03.01Keywords:
colonization, rhizosphere effect, mineral uptakeAbstract
Chickpea (Cicer arietinum L.) crop demands nitrogen fertilizer (NF) applied in excess caused lost soil fertility and environmental pollution. An alternative for solving this problem are: to reduce and to optimize NF in chickpea using inoculants based on plant growth promoting bacteria (PGPB) genus. Thus, the aim of this study was to analyze the response of chickpea to inoculation with Azotobacter vinelandii and Burkholderia cepacia at 50% reduced dose of NF. These PGPB were inoculated chickpea under an experimental design of randomized blocks. The response variables to measure the effect of PGPB on the legume were: the percent of germination, shoot and root phenotyping: plant height, root length and biomass: fresh and dry weight of shoot and root. Experimental data were analyzed by ANOVA and Tukey test. Results showed that a positive respond of chickpea to both PGPB on its germination as well as at seeding and flowering where chickpea had 0.82g total dry weight (TDW) of this value was statistically different and significant compared to chickpea treated by NF 100% not inoculated used as relative control (RC) with 0.71g TDW. This data suggests that chickpea optimized NF reduced dose by synergistic interaction of both genera PGPB in its root system. Which could to avoid in part soil lost fertility and environmental pollution for applying NF in excess.References
Armenta, A.D.; García, C.; Camacho, J.R.; Apodaca, M.A.; Montoya, L.G.; Nava, E. 2010. Biofertilizantes en el desarrollo agrícola de México. Revista Sociedad, Cultura y Desarrollo Sustentable 6: 51-56.
Camelo, M.; Vera, S.P.; Bonilla, R.R. 2011. Mecanismos de acción de las rizobacterias promotoras del crecimiento vegetal. Revista Ciencia y Tecnología Agropecuaria 12: 159-166.
Cárdenas, M.; Ortiz, R.; Echeverría, A.; Shagarodsky, T. 2012. Caracterización y selección agroproductiva de líneas de garbanzo (Cicer arietinum L) introducidas en Cuba. Cultivos Tropicales 33: 69-74.
Cárdenas-Navarro, R.; Sánchez-Yáñez, J.M.; Farías-Rodríguez, R.; Peña-Cabriales, J.J. 2004. Los aportes del Nitrógeno en la Agricultura. Revista Chapingo Serie Horticultura 10: 173-178.
Cello, F. di; Bevivino, A.; Chiarini, L.; Fani, R.; Paffetti, D.; Tabacchioni, S.; Dalmastri, C. 1997. Biodiversity of a Burkholderia cepacia population isolated from the maize rhizosphere at different plant growth stages. Applied Environmental Microbiology 63: 4485-4493.
Chanway, C.P.; Shishido, M.; Nairn, J.; Jungwirth, S.; Markham, J.; Xiaoy, G.; Holl, F.B. 2000. Endophytic colonization and field responses of hybrid spruce seedlings after inoculation with plant growth-promoting rhizobacteria. Forest Ecology Management. 133: 81-88.
Cutiño-Escalona, M. 2012. Inoculación de Azotobacter chroococcum en el cultivo del garbanzo (Cicer arietinum L). Revista Innovación Tecnológica 18: 1-7.
Freitas, L.R. de; Gennida, J.J. 1990. Plant growth promoting rhizobacteria for winter wheat. Canadian Journal Microbiology 36: 265-272.
Escobar, C.; Horna, Y.; Carreño, C.; Mendoza, G. 2011. Caracterización de cepas nativas de Azotobacter spp. y su efecto en el desarrollo de Lycopersicon esculentum Mill. “tomate” en Lambayeque. Scientia Agropecuaria 2: 39-49.
García, J.A.; Castillo, A.; Ramírez, M.E.; Rendón, G.; Larqué, M.U. 2001. Comparación de los procedimientos de Tukey, Duncan, Dunnett, HSU y Bechhofer para selección de medias. Agrociencia 35: 79-86.
García-González, M.M.; Farías-Rodriguez, R.; Peña-Cabriales, J.J.; Sánchez-Yáñez, J.M. 2005. Inoculación de trigo var. Pavón con Azospirillum sp. y Azotobacter bejerinckii. Terra Latinoamericana 23: 65-72.
González-Leyva, M.; González-Cruz, M.; Nápoles-Gallardo, E.; Baldaquín-Pagan, A. 2012. Efectividad de algunos biofertilizantes en el cultivo del garbanzo (Cicer Arietinum, L.) en un suelo Fersialítico Pardo Rojizo Mullido. Revista Innovación Tecnológica 18: 1-10.
Hernández, J.L.; Cubillos-Hinojosa, J.G.; Milian, P.E. 2012. Aislamiento de cepas de Rhizobium sp., asociados a dos leguminosas forrajeras en el Centro Biotecnológico del Caribe. Revista Colombiana de Microbiología Tropical 2: 51-62.
Hernández-Rodríguez, A.; Heydrich-Pérez, M.; Diallo, B.; Jaziri, M.E.; Vandeputte, O.M. 2010. Cell-free culture medium of Burkholderia cepacia improves seed germination and seedling growth in maize (Zea mays) and rice (Oryza sativa). Plant Growth Regulation 60: 191–197.
Piromyou, P.; Buranabanyat, B.; Tantasawat, P.; Tittabutr, P.; Boonkerd, N.; Teaumroong, N. 2011. Effect of plant growth promoting rhizobacteria (PGPR) inoculation on microbial community structure in rhizosphere of forage corn cultivated in Thailand. European Journal of Soil Biology 47: 44-54.
Prakash, J.; Yadav, J. 2012. Evaluation of plant growth promoting rhizobacteria and their effect on plant growth and grain yield of chickpea (Cicer arietinum L.) under sustainable agriculture. International Journal of Research in Engineering Computer and Social Sciences 2: 51-57.
Prakash, J.; Yadav, J.; Nath, K.; Kumar, A. 2013. Effect of indigenous Mesorhizobium spp and plant growth promoting rhizobacteria on yields and nutrients uptake of chickpea (Cicer arietinum L.) under sustainable agriculture. Ecological Engineering 51: 282–286.
Rodelas, B.; González-López, J.; Martínez-Toledo, M.V.; Pozo, C.; Salmerón, V. 1999. Influence of Rhizobium/Azotobacter and Rhizobium/Azospirillumcombined inoculation on mineral composition of faba bean (Vicia faba L.). Biology Fertilility Soils 29: 165–169.
Sánchez-Yáñez, J.M. 2007. Breve Tratado de Microbiología Agrícola Teoría y Práctica. Eds: Instituto de Investigaciones Químico Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, COSUSTENTA, S.A. de CV, Centro de Investigación y Desarrollo del Estado de Michoacán, Morelia, Mich. México. pp. 130-133, 136-138, 153-155.
Valverde, A.; Burgos, A.; Fiscella, T.; Rivas, R.; Velázquez, E.; Rodríguez-Barrueco, C.; Cervantes, E.; Chamber, M.; Igual, J.M. 2007. Differential effects of coinoculations with Pseudomonas jessenii PS06 (a phosphate-solubilizing bacterium) and Mesorhizobium ciceri C-2/2 strains on the growth and seed yield of chickpea under greenhouse and field conditions. Soil Biology Biochemical 1: 43-50.
* Autor para correspondencia.
E-mail: lmarquez@umich.mx (L. Márquez-Benavides).
Recibido 13 abril 2014.
Aceptado 17 junio 2014.
Published
How to Cite
Issue
Section
License
The authors who publish in this journal accept the following conditions:
a. The authors retain the copyright and assign to the magazine the right of the first publication, with the work registered with the Creative Commons attribution license, which allows third parties to use the published information whenever they mention the authorship of the work and the First publication in this journal.
b. Authors may make other independent and additional contractual arrangements for non-exclusive distribution of the version of the article published in this journal (eg, include it in an institutional repository or publish it in a book) as long as it clearly indicates that the work Was first published in this journal.
c. Authors are encouraged to publish their work on the Internet (for example, on institutional or personal pages) before and during the review and publication process, as it can lead to productive exchanges and a greater and faster dissemination of work Published (see The Effect of Open Access).
