In vitro effectiveness of chemical fungicides against Curvularia eragrostidis (Henn) J. A. Mey, causal agent of leaf spot disease in pineapple

Authors

  • Hugo Alberto García-Ordaz Facultad de Ciencias Biológicas y Agropecuarias, Universidad de Colima, km. 40 Autopista Colima-Manzanillo, Tecomán, Colima, C.P. 28930. https://orcid.org/0000-0002-6801-7162
  • Wilberth Chan-Cupul Facultad de Ciencias Biológicas y Agropecuarias, Universidad de Colima, km. 40 Autopista Colima-Manzanillo, Tecomán, Colima, C.P. 28930. https://orcid.org/0000-0001-8634-3618
  • Marco Tulio Buenrostro-Nava Facultad de Ciencias Biológicas y Agropecuarias, Universidad de Colima, km. 40 Autopista Colima-Manzanillo, Tecomán, Colima, C.P. 28930. https://orcid.org/0000-0003-3526-8667
  • Pedro Valadez-Ramírez Facultad de Ciencias Biológicas y Agropecuarias, Universidad de Colima, km. 40 Autopista Colima-Manzanillo, Tecomán, Colima, C.P. 28930. https://orcid.org/0000-0002-2837-3808

DOI:

https://doi.org/10.17268/sci.agropecu.2021.047

Keywords:

Ananas comosus, chemical control, doses response bioassay, daily growth rate, mycelial growth inhibition percentage, mean lethal concentration

Abstract

Curvularia eragrostidis (Ascomycota: Dothideomycetes) was recently reported as the causal agent of Pineapple Leaf Spot disease (MD2 hybrid) in Central Pacific Mexico. Therefore, the effectiveness of five chemical fungicides (System Cu®, Tilt® 250 CE, Volley® 88OL, Tecto 60® and Mancozeb® 80WP) at four concentrations (1.0%, 0.5%, 0.25% and 0.125%) was evaluated through a doses response bioassay. The DGR of C. eragrostidis was 1.5 cm/day. The fungicides Volley® and Tilt® at the four evaluated concentrations did not allow the growth of C. eragrostidis. The lowest DGR for System Cu®, Mancozeb® 80WP and Tecto® 60 was found at the dose of 1% with 0.13, 0.0 and 0.39 cm / day, respectively. The %IMG for Volley® and Tilt® 250 CE were 100% at all evaluated doses. For System Cu®, Mancozeb® 80WP and Tecto® 60, the %IMG ranged from 65.2 (0.125%) - 78.2 (1.0%), 62.9 (0.125%) - 100.0 (1.0%) and 57.0 (0.125%) - 62.0% (1.0%), respectively. The Probit model suggested an LC50 of 0.025%, 0.067% and 0.076% for System Cu®, Mancozeb® 80WP and Tecto® 60, respectively. In conclusion, the fungicides Volley® and Tilt® are highly effective against C. eragrostidis; on the other hand, the EC50 for System Cu®, Mancozeb® 80WP and Tecto® 60 against C. eragrostidis are reported for the first time.

References

Adaskaveg, J. E., Föster, H., Doug-Gubler, W., Teviotdale, B. L., & Thompson, D. F. (2005). Reduced-risk fungicides help manage brown rot and other fungal disease of stone fruit. California Agriculture, 59, 109-114.

Arahana, V., Borja, E., Salgado, C., Tobar, J., & Torres, M. L. (2012). Evaluación in vitro de fungicidas para el control de Monilinia spp. que afecta el cultivo de durazno (Prunus persica) en provincia de la Sierra Ecuatoriana. Avances en Ciencias e ingeniería, 4, 5-10.

Bahekar, A. M., Ingle, R. W., & Kendre, V. P. (2017). Efficacy of fungicides and bioagent against fungal pathogens of Aloe vera. International Journal of Chemical Studies. 5(4), 1540-1543.

Baldrian, P., & Gabriel, J. (2002). Intraspecific variability in growth response to cadmium of the wood-rotting fungus Piptoporus betulinis. Mycological Society of America, 94(3), 428-436.

Bartholomew, D. P., Rohrbach, K. G., & Evans, D. O. (2002). Pineapple cultivation in Hawaii. Fruits and Nuts, 7, 1-8.

Cristóbal-Alejo, J., Navarrete-Mapen, Z., Herrera-Parra, E., Mis-Mut, M., Tun-Suárez, J. M., & Ruiz-Sánchez, E. (2013). Hifomicetos asociados a plantas tropicales del estado de Yucatán, México: Identificación genérica y evaluación de fungicidas para su control. Revista de Protección Vegetal, 28(2), 138-144.

Ferreira, A. P. S., Pinho, D. B., Machado, A. R., & Pereira, O. L. (2014) First report of Curvularia eragrostidis causing postharvest rot on pineapple in Brazil. Plant Disease, 98(9), 1277.

Gao, S., Li, Y., Gao, J., Suo, Y., Fu, K., Li, Y., & Chen, J. (2014). Genome sequence and virulence variation-related transcriptome profiles of Curvularia lunata, an important maize pathogenic fungus. BMC Genomics, 15(1), 627.

Gaviria-Hernández, V., Patiño-Hoyos, L.F., & Saldarriaga-Cardona, A. (2013). Evaluación in vitro de fungicidas comerciales para el control de Colletotrichum spp., en mora de castilla. Corpoica Ciencia y Tecnología Agropecuaria, 14(1), 67-75.

García-Osorio, M. S., & Orozco-Gómez, B. A. (2016) Etiología de enfermeda-des en el cultivo de piña (Ananas comosus L. cv. Monte lirio) y pruebas de antagonismo “in vitro” con Trichoderma (Tesis de Licenciatura). Universidad Nacional Agraria, Managua, Nicaragua.

Hajar, N., Zainal, S., Nadzirah, K., Roha, A., Atikah, O., & Elida, T. (2012). Physicochemical properties analysis of three indexes pineapple (Ananas comosus) peel extract variety N36. APCBEE Procedia, 4, 115-121.

Hernández-Mansilla, A.A., Sierra-Peña, A., & Carr-Pérez, A. (2006). Evaluación in vitro del antagonismo de especies de Trichoderma sobre hongos fitopatógenos que afectan las vitroplantas de piña (Ananas Comosus (L.) Merr.). Fitosanidad, 10(2), 105-108.

Hernández-Mansilla A. A., Muiño-García, B. L., Rosón-Álvarez C., Casola-González, C., Porras-González, A. C., & López-Mayea A. (2010). Control químico de patógenos fungosos en piña (Ananas comosus (L.) Merrill) de vivero (II). Fitosanidad, 14(4), 235-239.

Korres, A., Buss, D., Ventura, J. & Fernandes, P. (2011). Candida krusei and Kloeckera apis inhibit the causal agent of pineapple fusariosis, Fusarium guttiforme. Fungal Biology, 115(12), 1251-1258.

Kotramma, C. A., Harlapur, S. I., Savita, C., & Basamma, R. H. (2018) Studies on evaluation of fungicides against Curvularia lunata in maize. International Journal of Agricultural Science, 14, 225-228.

Kusai, N. A., Azmi, M. M. Z., Zulkifly, S., Yusof, M. T., & Zainudin, N. A. I. M. (2015). Morphological and molecular characterization of Curvularia and related species associated with leaf spot disease of rice in Peninsular Malaysia. Rendiconti Lincei Scienze Fisiche e Naturali, 27, 205-214.

Lan-Cheng, M. Z., Yao-Jin, M. A., Ruan-Hong, M. C., & Yu-De, Y. (2016). Identification of Curvularia clavata causing leaf spot on pineapple (Ananas comosus) in China. Canadian Journal of Plant Pathology, 38, 250-253.

Maldonado-Michel, M. A., Muñiz-Valencia, R., Peraza-Campos, A. L., Pastor-Ramos, R. C., Valadez-Ramírez, P., Parra-Delgado, H., & Chan-Cupul W. (2021). Antifungal activity of Swietenia humilis (Meliaceae: Sapindales) seed extracts against Curvularia eragrostidis (Ascomycota: Dothideomycetes). Journal Plant Disease and Protection, 128, 471-479.

Manzo-Sánchez, G., Pérez-Ocón, R., Chan-Cupul, W., Silva-Jiménez, E., Sánchez-Rangel, J. A., Ayala-Zermeño, M. A., & Galindo-Velasco, E. (2018). Actividad antifúngica de extractos etanólicos de propóleo contra Mycosphaerella fijiensis: un estudio in vitro. Scientia Fungorum, 47, 13-24.

Ochoa-Martínez, D. L., Uriza-Ávila, D. E., Rojas-Martínez, R. I., & Rodríguez-Martínez, D. 2016. Detección de Pineapple mealybug wilt-associated virus 1 y 3 en México. Revista Mexicana de Fitopatología, 34, 131-141.

Rohrbach, K. G., & Schmitt, D. 2003. Diseases of pineapple. In: Ploetz, R.C. (ed.), Diseases of Tropical Fruit Crops (pp. 443-464). CABI: Wallingford, Oxon, UK.

SAS. (2002). Statistical Analysis System. Version 9.0.

Sabando-Ávila, F., Molina-Atiencia, L. M., & Garcés-Fiallos, F. R. (2017). Trichoderma harzianum en pre-transplante aumenta el potencial agronómico del cultivo de piña. Revista Brasileira de Ciencias Agrarias, 12(4), 410-414.

Septembre-Malaterre, A., Stanislas, G., Douraguia, E., & Marie-Paule, G. (2016). Evaluation of nutritional and antioxidant properties of the tropical fruit’s banana, litchi, mango, papaya, passion fruit and pinea-pple cultivated in Reunion French Island. Food Chemistry, 212, 225-233.

Tekade, A., Koche, M., Kothikar, R., & Surpam, A. (2017). Efficacy of fungicides and bioagents against Curvularia lunata causing blight of coleus under laboratory conditions. Journal of Medicinal Plants, 5(2), 189-191.

Published

2021-08-20

How to Cite

García-Ordaz, H. A. ., Chan-Cupul, W. ., Buenrostro-Nava, M. T. ., & Valadez-Ramírez, P. . (2021). In vitro effectiveness of chemical fungicides against Curvularia eragrostidis (Henn) J. A. Mey, causal agent of leaf spot disease in pineapple. Scientia Agropecuaria, 12(3), 429-434. https://doi.org/10.17268/sci.agropecu.2021.047

Issue

Section

Original Articles

Most read articles by the same author(s)