Genetic variability of 29 Peruvian quinoa (Chenopodium quinoa Willd) accessions using AFLP markers and multivariate analysis
DOI:
https://doi.org/10.17268/sci.agropecu.2021.007Keywords:
Chenopodium quinoa Willd., Germplasm, AFLPs, genetic diversity, genetic variation, genetic variabilityAbstract
Aiming to determine the genetic variability of accessions of Chenopodium quinoa Willd. "Quinoa" from the Germplasm Bank of INIA - Ayacucho, analyze 29 accessions, which were characterized using AFLP molecular markers. The quality of the DNA extracted by the Doyle and modified Doyle method was optimal for all samples, obtaining modifications in a range of 100 - 400 ng / μl. In the standardization of the AFLP technique, it was determined that the proteins between 100 and 200 ng / μl of digested DNA were adequate to efficiently develop the AFLP technique, and it was also determined that the preamplification products that allowed the resolution amplification profiles variables were higher with a 1:4 ratio. A total of 7 primer combinations generated 220 DNA bands, being 201 polymorphic loci. Molecular data grouped the accessions into seven groups at a similarity of 0.81 ultrametric units, of which a group is made up of 23 accessions and the remaining six made up of a single accession. It was found that the mean genetic variance was 0.306 ± 0.011, with a Shannon information index of 0.463 ± 0.015, these results showed that in the 29 quinoa accessions it presents a high degree of genetic variability and there is no duplicate accession. The study determines the genetic relationship and is essential to raise the genetic improvement and conservation of quinoa.
References
Avalos, H. (2018). El uso de la tecnología y su relación con la cadena productiva de la quinua en los agricultores de la localidad de Cabana de la región de Puno 2016. Veritas et Scientia, 7, 781-787.
Barut, M., Nadeem, M. A., Karaköy, T., & Baloch, F. S. (2020). DNA fingerprinting and genetic diversity analysis of world quinoa germplasm using iPBS-retrotransposon marker system. Turk J Agric For, 44, 479-491.
Bazile, D., Pulvento, C., Verniau, A., Al-Nusairi, M., Ba, D., et al. (2016). Evaluaciones mundiales de la quinua: resultados preliminares de los proyectos de la FAO posteriores al Año Internacional de la Quinua en nueve países. Frontiers in Plants Science, 7, 1-18.
Bonámico, N., Aiassa, J., Ibañez, M., Di Renzo, M., Díaz, D., et al. (2004). Caracterización y clasificación de híbridos simples de maíz con marcadores SSR1. RIA, 33(2), 129-144.
Brenes, E. (2001). El cluster de quinua en Bolivia: Diagnóstico competitivo y recomendaciones estratégicas. Proyecto andino de competitividad. Instituto Centroamericano de Administración de Empresas. Bolivia, 1- 54.
Camacho, V. T., Maxted, N., Scholten, M., & Ford-Lloyd, B. (2005). Defining and identifying crop landraces. Plant Genetic Resources, 3, 373-384.
Chirinos-Arias, M., Jiménez, J., & Vilca-Machaca, L. (2015). Analysis of genetic variability among thirty accessions of Andean Lupin (Lupinus mutabilis Sweet) using ISSR molecular markers. Scientia Agropecuaria, 6, 17-30.
Christensen, D., Pratt, C., Pratt, P. T., Nelson, M. R., Stevens, E.N., et al. (2007). Review agronomy and horticulture. Crop sciences, 12, 12-16.
CIP. Centro Internacional de la Papa. (2017). Protocolos de marcadores moleculares: Aplicaciones de los marcadores moleculares en la conservación y manejo de la agrobiodiversidad. Recuperado de: https://cgspace.cgiar.org/handle/10568/100669
Cóndor, R. (2013). Análisis de la variabilidad genética del olluco (Ullucus tuberosus caldas) de tres comunidades campesinas de Cusco y dos comunidades campesinas de Huánuco. (Tesis de maestría). Universidad Nacional Agraria La Molina, Perú.
Costa, S. (2014). Variabilidad genética de Chenopodium quinoa Willd en el Noroeste Argentino y su relación con la dispersión de la especie. (Tesis de doctorado). Universidad Nacional de la Plata, Argentina.
Doyle, J. J., & Doyle, J. L. (1990). Isolation of DNA from small amounts of plant tissues. Focus, 12, 13-15.
FAO (2018). El estado de la seguridad alimentaria y la nutrición en el mundo 2018. Food & Agriculture Org. Recuperado de: http://www.fao.org/3/I9553ES/i9553es
Florido, M., Arencibia, A., Plana, D., Alvárez M., López, J., & Lara R. (2007). Análisis de la diversidad genética en tomate (Solanum L. Sección Lycopersicon Sub especie Lycopersicon) utilizando AFLP. Cultivos Tropicales., 28, 83-87.
Hadipour, S., Kazemitabar, K., Yaghini, H., & Dayani, S. (2020). Diversidad genética y diferenciación de especies de plantas medicinales Amapola persa (Papaver bracteatum L.) usando marcadores AFLP e ISSR. Ecological Genetics and Genomics, 16, 100058.
Hossein-Pour, A., Haliloglu, K., Ozkan, G., & Tan, M. (2019). Genetic diversity andpopulation structure of quinoa (Chenopodium quinoa Willd.) using ipbs-retrotransposons markers. Aapplied Ecology and Environmental Research, 17(2), 1899-1911.
Jaccard, P. (1908). Nouvelles rescherches sur la distribution florale. Bull. Soc. Vaud. Sci. Nat., 44, 223-270.
Johnson, D. E. (2004). Métodos multivariados aplicados al análisis estadístico. Ciudad de México, México: Edit. International Thomson Editores.
Lema-Rumińska, J., Miler, N., & Gęsiński, K. (2018). Identification of new polish lines of chenopodium quinoa (willd.) by spectral analysis of pigments and a confirmation of genetic stability with scot and rapid markers. Acta Sci. Pol. Hortorum Cultus, 17(1), 75-86.
Nolasco, O., Cruz, W., Santa Cruz, C., & Gutiérrez A. (2013). Evaluación del polimorfismo de ADN de seis variedades de Chenopodium quinoa Willd, utilizando AFLP. The Biolist, 11, 277-286.
Peiretti, P., Gai, F., & Tassone, S. (2013). Fatty acid profile nutritive value of quinoa (Chenopodium quinoa Willd.) seeds and plants at different growth stages. Animal Feed Science and Technology, 183, 56-61.
Rosales, G., & Mercado, W. (2020). Efecto de los cambios en el precio de los alimentos sobre el consumo de la quinua y la seguridad alimentaria rural en el Perú. Scientia Agropecuaria, 11(1), 83-93.
Saad-Allah, K. M., & Youssef, M. S. (2018). Caracterización fitoquímica y genética de cinco genotipos de quinua (Chenopodium quinoa Willd.) introducidos en Egipto. Physiol Mol Biol Plants, 24, 617–629.
Saldaña, G. A., & Salazar, E. G. (2007). Aislamiento de ADN de calidad para la amplificación al azar de ADN polimórfico de mango. Agron Trop, 57, 281-286.
Vos, P., Hogers, R., Bleeker, M., Reijans, T., Van de Lee, T., et al. (1995). AFLP: a new technique for DNA fingerprinting. Nucleic Acids Research, 23, 4407-4414.
Wang, D., Caoa, D., Yaoa, Y., Wang J., Lia Z., et al. (2020). Understanding the chemical foundation and genetic mechanism of the black grain trait in quinoa by integrating metabolome and transcriptome analyses. Biotechnology & Biotechnological Equipment, 34 (1), 1095-1103.
Wu, X., Duan L., Chen, Q., & Zhang, D. (2020). Genetic diversity, population structure, and evolutionary relationships within a taxonomically complex group revealed by AFLP markers: A case study on Fritillaria cirrhosa D. Don and closely related species. Global Ecology and Conservation, 24, e01323.
Xiang-Rong, F., Wu-Chao, W., Li, C., Wei, L., & Yuan-Yuan C. (2021). Genetic relationship among 12 Trapa species/varietas from Yangtze River Basin revealed by AFLP markers. Aquatic Botany, 168, 103320.
Zhu, J., Gale, M. D., Quarrie, S., Jackson M. T., & Bryan, G. L. (1998). AFLP markers for study of rice biodiversity. Theoretical and Applied Genetics, 96, 602 - 611.
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 Paula García-Godos, Judith M. Cueva-Castillo
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
The authors who publish in this journal accept the following conditions:
a. The authors retain the copyright and assign to the magazine the right of the first publication, with the work registered with the Creative Commons attribution license, which allows third parties to use the published information whenever they mention the authorship of the work and the First publication in this journal.
b. Authors may make other independent and additional contractual arrangements for non-exclusive distribution of the version of the article published in this journal (eg, include it in an institutional repository or publish it in a book) as long as it clearly indicates that the work Was first published in this journal.
c. Authors are encouraged to publish their work on the Internet (for example, on institutional or personal pages) before and during the review and publication process, as it can lead to productive exchanges and a greater and faster dissemination of work Published (see The Effect of Open Access).
