Incorporation of nisin and oregano essential oil in cow’s milk to improve the quality of fresh cheese

John Víctor  Cama-Curasi; Erick  Saldaña; Melina L. M  Cruzado-Bravo; Carmen M. S.  Ambrosio; Jhony  Mayta-Hancco

doi:10.17268/sci.agropecu.2022.033

Autores/as

John Víctor Cama-Curasi Escuela Profesional de Ingeniería Agroindustrial, Universidad Nacional de Moquegua, Prolongación calle Ancash s/n, Moquegua 18001.
Erick Saldaña Escuela Profesional de Ingeniería Agroindustrial, Universidad Nacional de Moquegua, Prolongación calle Ancash s/n, Moquegua 18001.
Melina L. M Cruzado-Bravo Universidad Nacional Autónoma de Chota, Facultad Ciencias Agrarias, Escuela Ingeniería Agroindustrial, Chota, Cajamarca.
Carmen M. S. Ambrosio Universidad Privada del Norte
Jhony Mayta-Hancco Escuela Profesional de Ingeniería Agroindustrial, Universidad Nacional de Moquegua, Prolongación calle Ancash s/n, Moquegua.

DOI:

https://doi.org/10.17268/sci.agropecu.2022.033

Palabras clave:

Bovine milk, nisin, cheese, antibacterial activity, sensory analysis

Resumen

Firstly, this study aimed to determine the effect of adding nisin (0, 250 and 500 IU mL^-1) and oregano essential oil (OEO) (0, 0.025% and 0.05%), alone or in combination, on Staphylococcus aureus load in cow’s milk. Next, it was evaluated the sensory, physicochemical and microbiological quality of fresh cheese (FC) manufactured with milk added of nisin + OEO (C1) or nisin alone (C2) compared to a control cheese (C0, milk without the addition of nisin or OEO). Results showed that adding nisin effectively reduced the S. aureus count in milk in a similar manner to adding way the combination of nisin + OEO compared to the control. FC quality evaluation results revealed that C1 and C2 did not alter the physicochemical quality of FC (pH, fat and total solids content) during storage (0-8 days) compared to the control. Furthermore, C1 and C2 reduced in a similar manner aerobic mesophilic bacteria and Enterobacteriaceae count in FC compared to the control during storage. Sensory evaluation showed that consumers did not value FC added of nisin + OEO (C1) due to its intense aroma of oregano, rating it with the lowest global quality.

Citas

Akarca, G. (2019). Composition and antibacterial effect on food borne pathogens of Hibiscus surrattensis L. calyces essential oil. Industrial Crops and Products, 137, 285-289.

Amatiste, S., Sagrafoli, D., Giacinti, G., Rosa, G., Carfora, V., et al. (2014). Antimicrobial activity of essential oils against Staphylococcus aureus in fresh sheep cheese. Italian Journal of Food Safety, 3, 1696.

Arqués, J. L., Rodríguez, E., Nuñez, M., & Medina, M. (2011). Combined effect of reuterin and lactic acid bacteria bacteriocins on the inactivation of food-borne pathogens in milk. Food Control, 22, 457-461.

Asensio, C. M., Grosso, N. R., & Rodolfo Juliani, H. (2015). Quality preservation of organic cottage cheese using oregano essential oils. LWT - Food Science and Technology, 60(2, Part 1), 664-671.

Ashraf, M. N., Sameh, A., & Eman, K. A. S. (2020). Low Salt Soft Cheese; Compositional Quality and Incidence of Aerobic Spore Forming Bacteria. AJVS, 66(2), 48.

Ben Jemaa, M., Falleh, H., Neves, M. A., Isoda, H., Nakajima, M., & Ksouri, R. (2017). Quality preservation of deliberately contaminated milk using thyme free and nanoemulsified essential oils. Food Chemistry, 217, 726-734.

Ben Jemaa, M., Falleh, H., Saada, M., Oueslati, M., Snoussi, M., & Ksouri, R. (2018). Thymus capitatus essential oil ameliorates pasteurization efficiency. Journal of Food Science and Technology, 55(9), 3446-3452.

Boskovic, M., Djordjevic, J., Glisic, M., Ciric, J., Janjic, J., et al. (2020). The effect of oregano (Origanum vulgare) essential oil on four Salmonella serovars and shelf life of refrigerated pork meat packaged under vacuum and modified atmosphere. Journal of Food Processing and Preservation, 44(1), e14311.

Bovo Campagnollo, F., & Sant’Ana, A. S. (2022). Cheese: Public Health Aspects. In P. L. H. McSweeney & J. P. McNamara (Eds.), Encyclopedia of Dairy Sciences (Third Edition) (pp. 101-111). Oxford: Academic Press.

Carocho, M., Morales, P., & Ferreira, I. C. F. R. (2015). Natural food additives: Quo vadis? Trends in Food Science & Technology, 45(2), 284-295.

Castro, G., Valbuena, E., Bríñez, W., Sánchez, E., Vera, H., & Tovar, A. (2009). Comparación del empleo de nisina y cultivos de lactococcus lactis subsp. Lactis para la biopreservación de queso blanco. Revista Científica, 19(2), 201-123.

Chambers, J. V. (2002). The Microbiology of Raw Milk. In R. K. Robinson (Ed.), Dairy Microbiology Handbook: The Microbiology of Milk and Milk Products (3rd ed., pp. 39-90): John Wiley and Sons.

Coelho, M. C., Silva, C. C. G., Ribeiro, S. C., Dapkevicius, M. L. N. E., & Rosa, H. J. D. (2014). Control of Listeria monocytogenes in fresh cheese using protective lactic acid bacteria. International Journal of Food Microbiology, 191, 53-59.

Dai, J., Wu, S., Huang, J., Wu, Q., Zhang, F., et al. (2019). Prevalence and Characterization of Staphylococcus aureus Isolated from Pasteurized Milk in China. Frontiers in Microbiology, 10, 641.

de Arauz, L. J., Jozala, A. F., Mazzola, P. G., & Vessoni Penna, T. C. (2009). Nisin biotechnological production and application: a review. Trends in Food Science & Technology, 20(3), 146-154.

de Souza, G. T., de Carvalho, R. J., de Sousa, J. P., Tavares, J. F., Schaffner, D., de Souza, E. L., & Magnani, M. (2016). Effects of the Essential Oil from Origanum vulgare L. on Survival of Pathogenic Bacteria and Starter Lactic Acid Bacteria in Semihard Cheese Broth and Slurry. Journal of Food Protection, 79(2), 246-252.

El-Sayed, H. S., & El-Sayed, S. M. (2021). A modern trend to preserve white soft cheese using nano-emulsified solutions containing cumin essential oil. Environmental Nanotechnology, Monitoring & Management, 16, 100499.

European Food Safety Authority (EFSA). (2021). Foodborne outbreaks report 2020. https://www.efsa.europa.eu/en/ microstrategy/FBO-dashboard

Everett, D. W., & Auty, M. A. E. (2008). Cheese structure and current methods of analysis. International Dairy Journal, 18(7), 759-773.

Falleh, H., Ben Jemaa, M., Saada, M., & Ksouri, R. (2020). Essential oils: A promising eco-friendly food preservative. Food Chemistry, 330, 127268.

FAO/WHO. (2011). Group standard for unripened cheese including fresh cheese. Codex Stan 221-2001. In Milk and Milk Products (2nd ed., pp. 67-72). Rome, Italy: Food and Agriculture Organization of the United Nations (FAO) and World Health Organization (WHO).

FAO/WHO. (2021). Codex Alimentarius: General Standard for food additives. Codex Stan 192-1995.

Farkye, N. Y. (2004). Cheese technology. International Journal of Dairy Technology, 57(2-3), 91-98.

Felicio, B. A., Pinto, M. S., Oliveira, F. S., Lempk, M. W., Pires, A. C. S., & Lelis, C. A. (2015). Effects of nisin on Staphylococcus aureus count and physicochemical properties of Minas Frescal cheese. Journal of Dairy Science, 98, 4364-4369.

Fernandes, R. V. d. B., Guimarães, I. C., Ferreira, C. L. R., Botrel, D. A., Borges, S. V., & de Souza, A. U. (2017). Microencapsulated Rosemary (Rosmarinus officinalis) Essential Oil as a Biopreservative in Minas Frescal Cheese. Journal of Food Processing and Preservation, 41(1), e12759.

Fontecha, J., Peláez, C., Juárez, M., Requena, T., Gómez, C., & Ramos, M. (1990). Biochemical and Microbiological Characteristics of Artisanal Hard Goat's Cheese. Journal of Dairy Science, 73(5), 1150-1157.

Fox, P. F., Uniacke-Lowe, T., Mc Sweeney, P. L. H., & O’Mahony, J. A. (2015). Water in Milk and Dairy Products. In Dairy Chemistry and Biochemistry (pp. 299-320). Cham: Springer International Publishing.

Gharsallaoui, A., Joly, C., Oulahal, N., & Degraeve, P. (2016). Nisin as a Food Preservative: Part 2: Antimicrobial Polymer Materials Containing Nisin. Critical Reviews in Food Science and Nutrition, 56(8), 1275-1289.

Granato, D., de Araújo Calado, V. M., & Jarvis, B. (2014). Observations on the use of statistical methods in Food Science and Technology. Food Research International, 55, 137-149.

Han, P., Fark, T., de Wijk, R. A., Roudnitzky, N., Iannilli, E., Seo, H.-S., & Hummel, T. (2019). Modulation of sensory perception of cheese attributes intensity and texture liking via ortho- and retro-nasal odors. Food Quality and Preference, 73, 1-7.

Hebishy, E. (2013). Application of ultra high-pressure (UHPH) in the production of submicron/nano-oil-in water emulsions using vegetable oils and milk proteins as emulsifiers (Doctoral thesis). Universitat Autònoma de Barcelona

Hnosko, J., Clark, S., & Van Hekken, D. (2009). Latin American Cheeses. In S. Clark, M. Costello, M. Drake, & F. Bodyfelt (Eds.), The Sensory Evaluation of Dairy Products (pp. 489-504). New York, NY: Springer US.

Hyldgaard, M., Mygind, T., & Meyer, R. (2012). Essential Oils in Food Preservation: Mode of Action, Synergies, and Interactions with Food Matrix Components. Frontiers in Microbiology, 3, 12.

IDF. (2004). Cheese and processed cheese - Determination of the total solids content. IDF Standard 004:2004. Brussels, Belgium. In International Dairy Federation (IDF).

ISO. (1975). Cheese - Determination of fat content - van Gulik method. ISO Standard 3433. Leusden, Netherlands. In International Standards Organization (ISO).

D’Amico, D., & Donnelly, C. W. (2017). Growth and Survival of Microbial Pathogens in Cheese. In P. L. H. McSweeney, P. F. Fox, P. D. Cotter, & D. W. Everett (Eds.), Cheese (Fourth Edition) Chapter 22 (pp. 573-594). San Diego: Academic Press.

Ju, J., Xie, Y., Guo, Y., Cheng, Y., Qian, H., & Yao, W. (2019). The inhibitory effect of plant essential oils on foodborne pathogenic bacteria in food. Critical Reviews in Food Science and Nutrition, 59(20), 3281-3292.

Kilcast, D. (2013). 1 - Measurement of the sensory quality of food: an introduction. In D. Kilcast (Ed.), Instrumental Assessment of Food Sensory Quality (pp. 1-26): Woodhead Publishing.

Klein, G., Rüben, C., & Upmann, M. (2013). Antimicrobial Activity of Essential Oil Components Against Potential Food Spoilage Microorganisms. Current Microbiology, 67(2), 200-208.

Małaczewska, J., & Kaczorek-Łukowska, E. (2021). Nisin—A lantibiotic with immunomodulatory properties: A review. Peptides, 137, 170479.

Mariod, A. A. (2016). Chapter 13 - Effect of Essential Oils on Organoleptic (Smell, Taste, and Texture) Properties of Food. In V. R. Preedy (Ed.), Essential Oils in Food Preservation, Flavor and Safety (pp. 131-137). San Diego: Academic Press.

Mayta-Hancco, J., Trujillo, A.-J., Zamora, A., & Juan, B. (2019). Effect of ultra-high pressure homogenisation of cream on the physicochemical and sensorial characteristics of fat-reduced starter-free fresh cheeses. LWT - Food Science and Technology, 110, 292-298.

Melo, A. M. d., Turola Barbi, R. C., Souza, W. F. C. d., Luna, L. C., de Souza, H. J. B., et al. (2020). Microencapsulated lemongrass (Cymbopogon flexuosus) essential oil: A new source of natural additive applied to Coalho cheese. Journal of Food Processing and Preservation, 44(10), e14783.

Mishra, A. P., Devkota, H. P., Nigam, M., Adetunji, C. O., Srivastava, N., et al. (2020). Combination of essential oils in dairy products: A review of their functions and potential benefits. LWT, 133, 110116.

Nassib, T. A., Darwish, M. S., Abdelbaky, I., & Motee, D. (2018). Prevalence of Spore-Forming Bacteria Associated with the Processing of Milk and Dairy Products Processing in Mansoura City. Journal of Food and Dairy Sciences, 9(7), 257-261.

Olmedo, R. H., Nepote, V., & Grosso, N. R. (2013). Preservation of sensory and chemical properties in flavoured cheese prepared with cream cheese base using oregano and rosemary essential oils. LWT - Food Science and Technology, 53(2), 409-417.

Pandey, A. K., Kumar, P., Singh, P., Tripathi, N. N., & Bajpai, V. K. (2017). Essential Oils: Sources of Antimicrobials and Food Preservatives. Frontiers in Microbiology, 7, 2161.

Passarinho, A. T. P., Dias, N. F., Camilloto, G. P., Cruz, R. S., Otoni, C. G., Moraes, A. R. F., & Soares, N. d. F. F. (2014). Sliced Bread Preservation through Oregano Essential Oil-Containing Sachet. Journal of Food Process Engineering, 37(1), 53-62.

Perin, L. M., Pereira, J. G., Bersot, L. S., & Nero, L. A. (2019). The Microbiology of Raw Milk. In L. A. Nero & A. F. D. Carvalho (Eds.), Raw Milk. Balance Between Hazards and Benefits (pp. 45-64): Elsevier.

Pimentel-Filho, N. d. J., Mantovani, H. C., de Carvalho, A. F., Dias, R. S., & Vanetti, M. C. D. (2014). Efficacy of bovicin HC5 and nisin combination against Listeria monocytogenes and Staphylococcus aureus in fresh cheese. International Journal of Food Science and Technology, 49, 416-422.

Pinto, M. S., de Carvalho, A. F., Pires, A. C. D. S., Campos Souza, A. A., Fonseca da Silva, P. H., et al. (2011). The effects of nisin on Staphylococcus aureus count and the physicochemical properties of Traditional Minas Serro cheese. International Dairy Journal, 21(2), 90-96.

R Core Team. (2021). R: A Language and Environment for Statistical Computing (Version Vienna, Austria). https://www.R-project.org/: R Foundation for Statistical Computing.

Rodriguez-Garcia, I., Silva-Espinoza, B. A., Ortega-Ramirez, L. A., Leyva, J. M., Siddiqui, M. W., et al. (2016). Oregano Essential Oil as an Antimicrobial and Antioxidant Additive in Food Products. Critical Reviews in Food Science and Nutrition, 56(10), 1717-1727.

Saldaña, E., Behrens, J. H., Serrano, J. S., Ribeiro, F., de Almeida, M. A., & Contreras-Castillo, C. J. (2015). Microstructure, texture profile and descriptive analysis of texture for traditional and light mortadella. Food Structure, 6, 13-20.

Stratakos, A. C., & Koidis, A. (2016). Chapter 4 - Methods for Extracting Essential Oils. In V. R. Preedy (Ed.), Essential Oils in Food Preservation, Flavor and Safety (pp. 31-38). San Diego: Academic Press.

Teixeira, B., Marques, A., Ramos, C., Serrano, C., Matos, O., et al. (2013). Chemical composition and bioactivity of different oregano (Origanum vulgare) extracts and essential oil. Journal of the science of food and agriculture, 93(11), 2707-2714.

Tornambé, G., Cornu, A., Verdier-Metz, I., Pradel, P., Kondjoyan, N., et al. (2008). Addition of Pasture Plant Essential Oil in Milk: Influence on Chemical and Sensory Properties of Milk and Cheese. Journal of Dairy Science, 91(1), 58-69.

Ulpathakumbura, C. P., Ranadheera, C. S., Senavirathne, N. D., Jayawardene, L. P. I. N. P., Prasanna, P. H. P., & Vidanarachchi, J. K. (2016). Effect of biopreservatives on microbial, physico-chemical and sensory properties of Cheddar cheese. Food Bioscience, 13, 21-25.

Vásquez, V., Salhuana, J. G., Jimenez, L. A., & Abanto, L. M. (2018). Evaluación de la calidad bacteriológica de quesos frescos en Cajamarca. Ecología Aplicada, 17(1), 45-51.

Walstra, P., Wouters, J. T. M., & Geurts, T. J. (2005). Dairy Science and Technology (2nd ed.): CRC Press.

Yoon, J. I., Bajpai, V. K., & Kang, S. C. (2011). Synergistic effect of nisin and cone essential oil of Metasequoia glyptostroboides Miki ex Hu against Listeria monocytogenes in milk samples. Food and Chemical Toxicology, 49(1), 109-114.

Zamora, A., Ferragut, V., Quevedo, J. M., Guamis, B., & Trujillo, A. J. (2012). Ultra-high pressure homogenisation of milk: technological aspects of cheese-making and microbial shelf life of a starter-free fresh cheese. Journal of Dairy Research, 79, 168-175.