Efecto del aceite esencial de Cymbopogon citratus sobre propiedades fisicoquímicas en películas de quitosano


  • María Vázquez-Briones Departamento de Ingeniería Química, Alimentos y Ambiental, Universidad de las Américas Puebla. México.
  • José Guerrero-Beltrán Departamento de Ingeniería Química, Alimentos y Ambiental, Universidad de las Américas Puebla. México.



Palabras clave:

películas comestibles, quitosano, Cymbopogon citratus, propiedades mecánicas, color.


Se investigó el efecto de la incorporación de aceite esencial de zacate limón (Cymbopogon citratus), en concentraciones de 0, 200 y 400 ppm, en las propiedades fisicoquímicas (espesor, humedad, solubilidad, permeabilidad al vapor de agua, color, transparencia) y mecánicas (resistencia a la tensión y elongación) de películas de quitosano a dos concentraciones (1 y 3%). Los resultados mostraron un efecto significativo (p ≤ 0,05) en los valores del espesor de las películas a las dos concentraciones de quitosano. Se observó un efecto significativo (p ≤ 0,05) en los valores de humedad a concentraciones de 0, 200 y 400 ppm de aceite esencial. La adición de aceite esencial en películas de quitosano mostró un efecto significativo (p ≤ 0,05) en los valores de permeabilidad al vapor de agua. La concentración de quitosano mostró un efecto significativo (p ≤ 0,05) en transparencia y en los parámetros de color L*, a*, b*. Al variar la concentración de quitosano se observó un incremento significativo (p ≤ 0,05) en los valores de resistencia a la tensión de las películas de quitosano.


Abdollahi, M.; Rezaei, M.; Farzi, G. 2012. Improvement of active chitosan film properties with rosemary essential oil for food packaging. International Journal of Food Science and Technology 47: 847–853.

Andreuccetti, C.; Carvalho, R.; Galicia, G.T.; Martínez, B.F.; Grosso, C.R.F. 2011. Effect of surfactants on the functional properties of gelatin-based edible films. Journal of Food Engineering 103: 129–136.

ASTM (American Society for Testing and Materials). 1996. Standard test methods for water vapor transmission of materials E96–80. En: Annual book of ASTM. Philadelphia, PA. pp: 771–778.

Aydt, T.P.; Weller, C.; Testing, R.F.1991. Mechanical and barrier properties of edible corn and wheat protein films. American Society of Agricultural Engineers 34: 207-211.

Bassolé, I.H.N.; Lamien-Meda, A.; Bayala, B.; Obame, L.C.; Ilboudo, A.J.; Franz, C.; Dicko, M.H. 2011. Chemical composition and antimicrobial activity of Cymbopogon citratus and Cymbopogon giganteus essential oils alone and in combination. Phytomedicine 18: 1070-1074.

Cháfer, M.; Sánchez-González, L.; González-Martínez, Ch.; Chiralt, A. 2012. Fungal decay and shelf life of oranges coated with chitosan and bergamot, thyme, and tea tree essential oils. Journal of Food Science 77: E182–E18.

Cissé, M.; Montet, D.; Loiseau, G.; Ducamp-Collin, M.N. 2012. Influence of the concentrations of chitosan and glycerol on edible film properties showed by response surface methodology. Journal of Polymers and the Environment 20: 830–837.

Da Silva, M.A.; Bierhalz, A.C.K.; Kieckbusch, T.G. 2012. Modelling natamycin release from alginate/chitosan active films. International Journal of Food Science and Technology 47: 740–746.

Deng, Y.; Zhu, L.W.; Luo, W.; Xiao, C.L.; Song, X.Y.; Chen, J.S. 2009. Changes in physical properties of chitosan films at subzero temperatures. Italian Journal of Food Science 21: 487–498.

Duan, J.; Jiang, Y.; Zhao, Y. 2011. Chitosan-whey protein isolate composite films for encapsulation and stabilization of fish oil containing ultra pure omega-3 fatty acids. Journal of Food Science 76: C133-C141.

Elgadir, M.A.; Uddin, M.S.; Ferdosh, S.; Adam, A.; Chowdhury, A.J.K.; Sarker, M.Z.I. 2015. Impact of Chitosan composites and chitosan nanoparticle composites on various drug delivery systems: A review. Journal of Food and Drug Analysis 23: 619-629.

Eum, H.; Hwang, D.; Linke, M.; Lee, S.; Zude, M. 2009. Influence of edible coating on quality of plum (Prunus salicina Lindl. cv. ‘Sapphire’). European Food Research Technology 229: 427-434.

Fernández-Pan, I.; Ziani, K.; Pedroza-Islas, R.; Maté, J.I. 2010.

Effect of Drying conditions on the mechanical and barrier properties of films based on chitosan. Drying Technology 28: 1350-1358.

Goñi, P.; López, P.; Sánchez, C.; Gómez-Lus, R.; Becerril, R.; Nerín, C. Nerín. 2009. Antimicrobial activity in the vapour phase of a combination of cinnamon and clove essential oils. Food Chemestry 116: 982–989.

Hosseini, S.F.; Rezaei, M.; Zandi, M.; Ghavi, F.F. 2013. Preparation and funtional properties of fish gelatin-chitosan blend edible films. Food Chemistry 136: 1490-1495.

Jiang, Z.; Neetoo, H.; Chen, H. 2011. Control of Listeria monocytogenes on cold-smoked salmon using chitosan-based antimicrobial coatings and films. Journal of Food Science 76: M22–M26.

Jirukkakul, N.A. 2013. A study of Mu Yor sausage wraps using chitosan films incorporating garlic oil, lemon grass oil and galangal oil. International Food Research Journal 20: 1199–1204.

Kerch, G.; Korkhov, V. 2011. Effect of storage time and temperatura on structure, mechanical and barrier properties of chitosan-based films. European Food Research and Technology 232: 17-22.

Krkic, N.; Lazic, V.; Savatic, S.; Sojic, B.; Petrovic, L.; Suput, D. 2012a. Properties of chitosan-lamiated collagen film. Food Technology and Biotechnology 50: 483-489.

Krkic, N.; Lazic, V.; Petrovic, L.; Gvozdenovic, J.; Pejic, D. 2012b. Application of chitosan coating with oregano essential oil on dry fermented sausage. Journal of Food and Nutrition Research 51: 60-68.

Kundu, P.P.; Jindal, S.K.; Goswami, M. Goswami. 2013. Characterization and in vitro and in vivo evaluation of cross-linked chitosan films as implant for controlled release of citalopram. Bulletin of Materials Science 36: 175–182.

Kurt, A.; Kahyaoglu, T. 2014. Characterization of a new biodegradable edible film made from salep gluco-mannan. Carbohydrate Polymers 104: 50–58.

Leceta, I.; Arana, P.; Guerrero, P.; Cabak. 2014. Structure-moisture sorption relation in chitosan thin films. Materials Letters 129: 125-127.

Leerahawong, A.; Tanaka, M.; Okazaki, E.; Osako, K. 2011. Effects of plasticizer type and concentration on the physicochemical properties of edible film from squid Todarodes pacificus mantle muscle. Fisheries Science 77(6): 1061-1068.

López-Mata, M.A.; Ruiz-Cruz, S.; Silva-Beltrán, N.P.; Ornelas-Paz, J.J.; Ocaño-Higuera, V.M.; Rodríguez-Félix, F.; Cira-Chávez, L.A.; Toro-Sánchez, C.L.; Shirai, K. 2015. Physicochemical and Antioxidant Properties of Chitosan Films Incorporated with Cinnamon Oil. International Journal of Polymer Science 2015: 1-8.

Martelli, R.M.; Barros, T.T.; De Moura, R.M.; Maltoso, L.H.; Assis, O.B. 2013. Effect of chitosan nanoparticles and pectin content on mechanical properties and water vapor permeability of banana puree films. Journal of Food Science 78: N98–N103.

Mayachiew, P.; Devahasti, S. 2008. Comparative evaluation of physical properties of edible chitosan films prepared by different drying method. Drying Technology 26: 176–185.

De Moura, R.M.; Lorevice, V.M.; Mattoso, H.C.L.; Zucolotto, V. 2011. Highly stable, edible cellulose films incorporating chitosan nanoparticles. Journal of Food Science 76: N25-N29.

Nguefack, J.; Dongmo, J.B.L.; Dakole, C.D.; Leth, V.; Vismer, H.F.; Torp, J.; Nkengfack, A.E. 2009. Food preservative potential of essential oils and fractions from Cymbopogon citratus, Ocimum gratissimum and Thymus vulgaris against mycotoxigenic fungi. International Journal of Food Microbiology 131: 151–156.

Parikh, J.K.; Desai, M.A. 2011. Hydrodistillation of essential oil from Cymbopogon flexuosus. International Journal of Food Engineering 7: 1–8.

Pelissari, F.M.; Yamashita, F.; Grossmann, M.V.E. 2011. Extrusion parameters related to starch/chitosan active films properties. International Journal of Food Science and Technology 46: 702–710.

Peng, Y.; Yin, L.; Li, Y.2013. Combined effects of lemon essential oil and surfactants on physical and structural properties of chitosan films. International Journal of Food Science and Technology 48: 44–50.

Ramos, L. O.; Silva, I.S.; Soares, C.J.; Fernandes, C.J.; Poças, M.F.; Pintado, E.M.; Malcata, F.X. 2012. Features and performance of edible films, obtained from whey protein isolate formulated with antimi-crobial compounds. Food Research International 45: 351-361.

Rawdkuen, S.; Suthiluk, P.; Kamhangwong, K.; Benjakul, S. 2012. Mechanical, physico-chemical, and antimi-crobial properties of gelatin-based film incorporated with catechin-lysozyme. Chemistry Central Journal 6: 2-10.

Sánchez-García, C.; Cruz-Martín, M.; Alvarado-Capó, Y.; Pérez, M.; Medinilla, M. 2007. Evaluación del efecto del aceite esencial de Cymbopogon nardus para el control de microorganismos contaminantes del cultivo in vitro de plantas. Biotecnología Vegetal 7: 187–190.

Schaneberg, BT.; Khan, I.A. 2002. Comparison of extrac-tion methods for marker compounds in the essentil oil of lemon grass by GC. Journal of Agricultural and Food Chemistry 50: 1345-1349.

Shahidi, F.; Arachchi, J.K.V.; Jeon, Y. 1999. Food applica-tions of chitin and chitosans. Trends in Food Science & Technology 10: 37-51.

Souza, V.C.; Monte, M.L.; Pinto, L.A.A. Pinto A. 2011. Preparation of biopolymer film from chitosan modified with lipid fraction. International Journal of Food Science and Technology 46: 1856–1862.

Tofiño-Rivera, A.; Ortega-Cuadros, M.; Galvis-Pareja, DE.; Jiménez-Rios, H.; Merinie, L.J.; Martínez-Pabónf, M.C. 2016. Effect of Lippia alba and Cymbopogon citratus essential oils on biofilms of Streptococcus mutans and cytotoxicity in CHO cells. Journal of Ethnopharmacology 194: 749–754.

Vázquez-Briones, M.C.; Hernández, R.L.; Guerrero-Beltrán, J.A. 2015. Physicochemical and antioxidant properties of Cymbopogon citratus essential oil. Journal of Food Research 4: 36-45.

Wang, J.; Shang, J.; Ren, F.; Leng, X. 2010. Studio of the physical properties of whey protein: sericin protein-blended edible films. European Food Research and Technology 231: 109-116.

Wannissorn, B.; Jarikasem, S.; Soontorntanasart, T. 1996. Antifungal activity of lemon grass oil and lemon grass oil cream. Phytotherapy Research 10: 551-554.

Yuan, G.; Chen, X.; Li, D. 2017. Chitosan films and coatings containing essential oils: The antioxidant and antimi-crobial activity, and application in food systems. Food Research International 89: 117-128.

Zhong, Q.P.; Xia, W.S. 2008. Physicochemical properties of edible and preservative films from chitosan/cassava starch/gelatin blen plasticized with glicerol. Food Technology and Biotechnology 46: 262-269.

Received November 30, 2016.

Accepted October 16, 2017.

Corresponding author: angel.guerrero@udlap.mx (J. Guerrero-Beltrán).




Cómo citar

Vázquez-Briones, M., & Guerrero-Beltrán, J. (2017). Efecto del aceite esencial de Cymbopogon citratus sobre propiedades fisicoquímicas en películas de quitosano. Scientia Agropecuaria, 8(4), 401-409. https://doi.org/10.17268/sci.agropecu.2017.04.11



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