Inhibición de la embrionación in vitro de huevos de Toxocara canis expuestos al extracto hidroalcoholico del fruto de Punica granatum (Lythraceae)

Deysi Elizabeth Gerónimo Escobal; Hernán Rodríguez Quezada; César Augusto Jara Campos

Authors

Deysi Elizabeth Gerónimo Escobal Universidad Nacional de Trujillo, Av. Juan Pablo II s/n – Ciudad Universitaria, Trujillo, Perú. https://orcid.org/0009-0003-9853-1175
Hernán Rodríguez Quezada Universidad Nacional de Trujillo, Av. Juan Pablo II s/n – Ciudad Universitaria, Trujillo, Perú. https://orcid.org/0009-0001-6808-0209
César Augusto Jara Campos Universidad Nacional de Trujillo, Av. Juan Pablo II s/n – Ciudad Universitaria, Trujillo, Perú. https://orcid.org/0000-0002-8864-4876

Keywords:

Embrionación, extracto hidroalcohólico, Punica granatum, Toxocara canis

Abstract

Toxocara canis es un nematodo responsable de la toxocariasis, una zoonosis frecuente en niños de países en desarrollo. El tratamiento convencional con antiparasitarios sintéticos, aunque eficaz, presenta toxicidad, efectos adversos y riesgo de resistencia, lo que impulsa la búsqueda de alternativas como la fitoterapia. La cáscara de Punica granatum L. posee compuestos bioactivos con propiedades antiparasitarias, pero los estudios con material botánico del norte del Perú son limitados. Este estudio evaluó in vitro el efecto del extracto hidroalcohólico de la cáscara del fruto maduro de P. granatum (EHP) de Poroto (La Libertad, Perú) sobre la embrionación de huevos de T. canis. Los huevos se incubaron con EHP (0.5 y 1.0 mg/mL) y en formol salino al 2% (control) durante 15 días a 18-25 °C. El EHP inhibió la embrionación en un 91.3% (1.0 mg/mL) y 43.3% (0.5 mg/mL) (p<0.05). Se concluye que el EHP presenta actividad inhibitoria dosis dependiente, posiblemente asociada a flavonoides.

DOI: http://dx.doi.org/10.17268/rebiol.2024.44.01.05

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