Alternatives to pure chemical reagents: Use of soluble fertilizers in the in vitro propagation of Musa AAB cv. Hartón

Luis  Godoy-Montiel; María Eugenia  Romero-Román; Nataly  Herrera-Tamayo; Ronald Oswaldo  Villamar-Torres; Seyed  Mehdi Jazayer; Tefide  Kızıldeniz

doi:10.17268/agroind.sci.2025.02.03

Autores/as

Luis Godoy-Montiel Facultad de Ciencias Pecuarias y Biológicas, Universidad Técnica Estatal de Quevedo, Ecuador. https://orcid.org/0000-0002-0551-9974
María Eugenia Romero-Román Facultad de Ciencias Pecuarias y Biológicas, Universidad Técnica Estatal de Quevedo, Quevedo, Ecuador. https://orcid.org/0000-0003-2665-5366
Nataly Herrera-Tamayo Facultad de Ciencias Pecuarias y Biológicas, Universidad Técnica Estatal de Quevedo, Quevedo, Ecuador. https://orcid.org/0000-0002-9949-2775
Ronald Oswaldo Villamar-Torres Facultad de Ciencias Pecuarias y Biológicas, Universidad Técnica Estatal de Quevedo, Quevedo, Ecuador. https://orcid.org/0000-0003-2511-1789
Seyed Mehdi Jazayer ERIT PSII – Plant Science, Interactions and Innovation, Institut Agrosciences, Environnement, et Santé (AgES), Avignon Université, Avignon, France. https://orcid.org/0000-0003-2852-5063
Tefide Kızıldeniz Niğde Ömer Halisdemir University, Faculty of Agricultural Sciences and Technologies, Biosystem Engineering Department, Niğde, Turkiye https://orcid.org/0000-0002-5627-1307

DOI:

https://doi.org/10.17268/agroind.sci.2025.02.03

Palabras clave:

In vitro culture, Musa paradisiaca var, Hartón, plant biotechnology, Soluble commercial fertilizers, plant health

Resumen

In vitro propagation has emerged as a crucial technique in the propagation of plantain (Musa AAB cv. Hartón), a crop of high commercial demand in Ecuador, which allows the mass production of healthy and uniform plants, facilitating the rapid multiplication of cultivars with desirable characteristics, contributing significantly to crop health by eliminating pathogens and producing disease-free plants. To evaluate the use of soluble commercial fertilizers in the formulation of macronutrients in the in vitro propagation of plantain, the Student Test was applied with two treatments (chemical reagents and soluble fertilizers), each one including 10 observations. The corms were disinfected with 96% alcohol and a 1% sodium hypochlorite solution and cultured in MS medium. Ammonium nitrate, potassium nitrate, magnesium sulfate heptahydrate and potassium phosphate monobasic were used as soluble fertilizers, using molar equivalences to ensure the same amount of nutrients as the pure chemical reagents. The treatment with soluble fertilizers (Treatment 2) presented a higher percentage of phenolization (65%), fungal contamination (30%) and bacterial contamination (40%) compared to the treatment with chemical reagents. Bacterial contamination was controlled with the use of a bactericide. The use of highly soluble fertilizers promoted a faster phenological development, and after the breaking of apical dominance, the incidence of fungal contamination decreased, without presenting bacterial contamination. These findings suggest that soluble fertilizers may be a viable and effective alternative to pure chemical reagents in the in vitro propagation of Musa AAB cv. Hartón.

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