First report of Staphylococcus isolates identified by genomic analysis from rhizospheric soils of Capsicum annuum L. cv Piquillo

Autores

DOI:

https://doi.org/10.17268/sci.agropecu.2020.02.11

Palavras-chave:

Staphylococcus, 16s rRNA gene, Capsicum annuum L., rhizospheric soils.

Resumo

The genus Staphylococcus comprises many species which can be isolated from many sources and could display plant growth-promoting properties. Moreover, Capsicum species are important export crops in Peru, which have gained greater interest in recent years. Therefore, the objective of this research was to identify Staphylococcus isolates from rhizospheric soil samples of C. annuum cv. Piquillo in La Libertad, Peru. Bacterial isolates were identified by genomic analysis targeting the 16s rRNA gene. Bacteria were isolated from samples by serial dilutions and cultured in solid medium agar plates. Then, genomic DNA extraction from pure and morphologically distinct isolates, 16s rRNA gene amplification, sequencing and bioinformatic analysis were performed. We found four bacterial isolates from the genus Staphylococcus not previously reported in C. annuum rhizospheric soils: Isolate Ca2 and Ca5 which both match to Staphylococcus sp., isolate Ca6 to Staphylococcus arlettae and isolate Ca7 to Staphylococcus xylosus. Further studies to assess these isolates’ impact on crops as well as their potential applications in other fields of research such as antimicrobial development, food processing and pesticide biodegradation are recommended.

Referências

Agraria.pe. 2019. Se debe aprovechar atributos únicos de ajíes peruanos. Available in: https://agraria.pe/noticias/se-debe-aprovechar-atributos-unicos-de-ajies-peruanos-20128

Asaff-torres, A.; Armendáriz-ruiz, M.; Kirchmayr, M.; et al. 2017. Rhizospheric Microbiome Profiling of Capsicum annuum L. Cultivated in Transcribed Spacer 2 rRNA Amplicon. Genome Announcements 5(30): e00626-17.

At, K.; Karthikeyan, S.; Thanga, S. 2019. Occurrence and microbial degradation of fipronil residues in tropical highland rhizosphere soils of Kerala, India. Soil and Sediment Contamination: An International Journal 28(4): 360-379.

Bastos, M.; Ceotto, H.; Coelho, M.; et al. 2009. Staphylococcal Antimicrobial Peptides: Relevant Properties and Potential Biotechnological Applications. Current Pharmaceutical Biotechnology 10(1): 38-61.

Belgini, D.; Dias, R.; Siqueira, V.; et al. 2014. Culturable bacterial diversity from a feed water of a reverse osmosis system, evaluation of biofilm formation and biocontrol using phages. World Journal of Microbiology and Biotechnology 30(10): 2689-2700.

Berg, G.; Eberl, L.; Hartmann, A. 2005. The rhizosphere as a reservoir for opportunistic human pathogenic bacteria. Environmental Microbiology 7(11): 1673-1685.

Canovas, J.; Baldry, M.; Bojer, M.S.; et al. Cross-Talk between Staphylococcus aureus and Other Staphylococcal Species via the agr Quorum Sensing System. Front Microbiol. 7: 1733.

Chinakwe, E.; Nwogwugwu, N.; Ibekwe, V.; et al. 2019. Isolation and Evaluation of Bacteria Exhibiting Multiple Plant Growth Traits in the Rhizosphere of Yellow Bell Pepper (Capsicum chinense). Journal of Advances in Microbiology 14(4):1-6.

Correia, S.; Silva, V.; García-Díez, J.; et al. 2019. One Health Approach Reveals the Absence of Methicillin-Resistant Staphylococcus aureus in Autochthonous Cattle and Their Environments. Frontiers in Microbiology 10: 2735

De Vos, P. 2015. Bacillales. In: Bergey’s Manual of Systematics of Archaea and Bacteria. John Wiley & Sons, Inc. Belgium. Pp. 1-1.

González, A.; Almaraz, J.; Ferrera, R.; et al. 2017. Caracterización y selección de rizobacterias promotoras de crecimiento en plántulas de chile poblano (Capsicum annuum L.). Revista Internacional de Contaminacion Ambiental 33(3): 463-474.

Hall, T. 1999. BioEdit: A User-Friendly Biological Sequence Alignment Editor and Analysis Program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95-98.

Holguin, G. 1992. Two new nitrogen-fixing bacteria from the rhizosphere of mangrove trees: Their isolation, identification and in vitro interaction with rhizosphere Staphylococcus sp. FEMS Microbiology Letters 101(3): 207-216.

Jindal, S.; Dhaliwal, M.; Meena, O. 2020. Molecular advancements in male sterility systems of Capsicum: A review. Plant Breeding 139(1): 42-64.

Khoury, C.; Carver, D.; Barchenger, D.; et al. 2020. Modelled distributions and conservation status of the wild relatives of chile peppers (Capsicum L.). Diversity and Distributions 26(2): 209-225.

Kimura, M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution 16(2): 111-120.

Kozajda, A.; Jeżak, K.; Kapsa, A. 2019. Airborne Staphylococcus aureus in different environments - a review. Environmental Science and Pollution Research 26(34): 34741-34753.

Kumar, S.; Stecher, G.; Tamura, K. 2016. MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Molecular Biology and Evolution 33(7): 1870-1874.

Leiva, E.; Osorio, M.; Ramírez, R. 2013. Microorganismos asociados a la rizósfera de C cacao (Theobroma cacao L.) en condiciones de Bosque húmedo Premontano (Bh-PM). Suelos Ecuatoriales 43(1): 35-45.

López, Á.; Castellanos, M.; Pitre, L. 2009. Caracteri-zación bacteriana de la rizósfera del suelo asociado al manglar del delta del Río Ranchería, Riohacha, La Guajira. Suelos Ecuatoriales 39(1): 40-44.

Mandal, S.; Ghosh, A.; Pati, B. 2015. Dissemination of antibiotic resistance in methicillin-resistant Staphylococcus aureus and vancomycin-resistant S aureus strains isolated from hospital effluents. American Journal of Infection Control, 43(12), e87-e88.

MINAGRI-Ministerio de Agricultura y Riego. 2017. Plan de Desarrollo de las Especies del género del Género Capsicum 2018-2028. Available in: https://www.minagri.gob.pe/portal/download/pdf/marcolegal/normaslegales/resolucionesministeriales/2017/octubre/rm434-2017-minagri.pdf

MINAGRI-Ministerio de Agricultura y Riego; Pro-Inversión. n.d. Pimiento piquillo. Available at: https://www.minagri.gob.pe/portal/download/pdf/herramientas/organizaciones/dgca/pimiento-piquillo.pdf

Oh, W.; Jun, J.; Giri, S.; et al. 2019. Staphylococcus xylosus infection in rainbow trout (Oncorhynchus mykiss) as a primary pathogenic cause of eye protrusion and mortality. Microorganisms 7(9): 330.

Prithiviraj, B.; Bais, H.P.; Weir, T.; et al. 2005. Down Regulation of Virulence Factors of Pseudomonas aeruginosa by Salicylic Acid Attenuates Its Virulence on Arabidopsis thaliana and Caenorhabditis elegans. Infection and Immunity 5319-5328.

Saitou, N.; Nei, M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4(4): 406-425.

Schleifer, K.; Bell, J. 2015. Staphylococcus. In: Bergey’s Manual of Systematics of Archaea and Bacteria. John Wiley & Sons, Inc. Germany. Pp. 1-43.

Shahid, M.; Ahmed, T.; Noman, M.; et al. 2019. Non-pathogenic Staphylococcus strains augmented the maize growth through oxidative stress management and nutrient supply under induced salt stress. Annals of Microbiology 69(7): 727-739.

Stavropoulou, D.; De Maere, H.; Berardo, A.; et al. 2018. Pervasiveness of Staphylococcus carnosus over Staphylococcus xylosus is affected by the level of acidification within a conventional meat starter culture set-up. International Journal of Food Microbiology 274: 60-66.

Thompson, J.; Gibson, T.; Plewniak, F.; et al. 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 25(24): 4876-4882.

Tripodi, P.; Kumar, S. 2019. The Capsicum Crop: An Introduction. In: The Capsicum Genome. Springer, Cham. Switzerland. Pp. 1-8.

Publicado

2020-06-08

Como Citar

Asmat, C., Cruz-Valderrama, B., & Chaman, M. (2020). First report of Staphylococcus isolates identified by genomic analysis from rhizospheric soils of Capsicum annuum L. cv Piquillo. Scientia Agropecuaria, 11(2), 237-240. https://doi.org/10.17268/sci.agropecu.2020.02.11

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