Rhizospheric and phylloplane bacteria from Capsicum annuum: Uncovering candidates for biocontrol of Ralstonia solanacearum

Paola  Rodulfo-Acuña; Yonis  Hernández; Pedro  Terrero-Yepez; Bella Paiva; Edgloris Marys-Sarabia; Rafael Mejías-Herrera

doi:10.17268/sci.agropecu.2025.042

Autores/as

Paola Rodulfo-Acuña Estación Experimental Tropical Pichilingue, Instituto Nacional de Investigaciones Agropecuarias, Programa Nacional de Banano, Plátano y otras Musáceas, km 5 vía Quevedo, El Empalme, Cantón Mocache, Los Ríos, Ecuador. https://orcid.org/0009-0007-4697-2752
Yonis Hernández Facultad de Agronomía, Laboratorio de Bacterias Fitopatógenas, Universidad Central de Venezuela (UCV), Maracay, Venezuela. https://orcid.org/0000-0002-5838-6159
Pedro Terrero-Yepez Estación Experimental Tropical Pichilingue, Instituto Nacional de Investigaciones Agropecuarias, Programa Nacional de Banano, Plátano y otras Musáceas, km 5 vía Quevedo, El Empalme, Cantón Mocache, Los Ríos, Ecuador. https://orcid.org/0000-0002-4492-4577
Bella Paiva Laboratorio de Microbiologia Vegetal, Instituto de Estudios Avanzados (IDEA), Hoyo de la Puerta, Baruta, Venezuela. https://orcid.org/0009-0001-0466-589X
Edgloris Marys-Sarabia Laboratorio de Biotecnología y Virología Vegetal, Centro de Microbiología y Biología Celular, Instituto Venezolano de Investigaciones Científicas (IVIC), Caracas, Venezuela. https://orcid.org/0000-0002-4463-9207
Rafael Mejías-Herrera Facultad de Agronomía, Laboratorio de Bacterias Fitopatógenas, Universidad Central de Venezuela (UCV), Maracay, Venezuela. https://orcid.org/0000-0002-3431-1582

DOI:

https://doi.org/10.17268/sci.agropecu.2025.042

Palabras clave:

Ralstonia solanacearum, biocontrol, Pseudomonas, halo, inhibition

Resumen

Ralstonia solanacearum, the causative agent of bacterial wilt, is a major plant pathogen affecting many economically significant crops, including pepper (Capsicum annuum). This pathogen causes severe yield losses due to the limited effectiveness of current control measures. This study aimed to evaluate potential biocontrol agents for managing Ralstonia solanacearum by isolating and testing microorganisms from the rhizosphere and phylloplane of pepper plants. A total of 32 bacterial isolates were screened, and four strains showed the most pronounced antagonistic activity in vitro, producing inhibition zones ranging from 4.0 to 6.12 cm. The most effective isolates included three rhizospheric strains identified as Bacillus sp., Serratia sp., and Pseudomonas sp., and one phylloplane strain identified as Pseudomonas aeruginosa. These microorganisms effectively suppressed Ralstonia solanacearum growth under laboratory conditions and show strong potential as biocontrol agents for bacterial wilt in pepper crops.

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