Effect of explant density and volume of cultivation medium on in-vitro multiplication of blueberry (Vaccinium corymbosum L.) varieties "Brigitta" and "Legacy"

Authors

  • Mario Rodríguez Beraud Universidad Católica de Temuco, Temuco
  • Daniza Morales Ulloa Universidad Católica de Temuco, Temuco

DOI:

https://doi.org/10.17268/sci.agropecu.2015.01.03

Keywords:

in vitro culture, micropropagation, explants, shoots

Abstract

The objective of the investigation was to evaluate the in-vitro multiplication of two varieties of blueberry (Vaccinium corymbosum L.), "Brigitta" and "Legacy" in response to five explants densities (5, 10, 15, 20 and 25) and four flask volumes (10, 20, 30 and 40 mL) for cultivation. For both varieties the cultivation medium WPM (Woody Plant Medium) was used. The experiment was completely randomized with 20 treatments and 12 repetitions per treatment. After 45 days of cultivation we evaluated the height of shoots, number of shoots/explant, number of nodes/shoot and number of shoots/flask. Variety "Brigitta" had highest shoots at higher densities and flask volumes, while variety "Legacy" had the highest average shoot height with intermediate densities and high volumes. Regarding the number of shoots/explant, the volume of the medium had no influence on "Brigitta", however, higher plant densities affected this parameter. With variety "Legacy" the maximum number of shoots was achieved with lower plant densities and intermediate culture volumes per flask. In relation to the number of nodes per explant "Brigitta had lower numbers as compared to "Legacy", but with both varieties the number of nodes decresed with smaller volumes of medium in the flasks. For the number of shoots per flask, "Brigitta" responsed best at higher densities exceeding 40 shoots per flask. In contrast, "Legacy" produced maximum results at density of 25 explants in 30 mL of medium. It is concluded that for the optimum multiplication of both varieties the correct selection of both, the planting density and the volume of multiplication medium are important.

References

Aicha, N.; Mohamed, H.; Abdelmalek, E. 2014. In vitro clonal propagation through direct shoot organogenesis of thymus broussonetii – a vulnerable aromatic and medicinal plant species. International Journal of Pharmaceutical Research and Bio-Sciences 3(1): 425-439.

Aitken-Chrisie, J.; Kozai, T.; Smith, M. 1994. Automation and environmental control in plant tissue culture. Kluwer Academic Publishers. Dordrecht, The Netherlands.

Assis, K.; Pereira, F.; Rodríguez, J.; Silva, F.; Silva, J.; dos Santos, S. 2012. In vitro cultivation of Anacardium othonianum Rizz.: effects of salt concentration and culture medium volume. Acta Scientiarum. Agronomy 34(1): 77-83.

Bravo, J. 2014. Boletín frutícola. Avance noviembre 2014. Oficina de Estudios y Políticas Agrarias (ODEPA), Ministerio de Agricultura. Santiago, Chile. 28 p.

Buzeta, A. 1997. Chile: berries para el 2000. Departamento Agroindustrial. Fundación Chile. Santiago, Chile.

Cao, X.; Hammerschlag, F.A. 2000. Improved shoot organogenesis from leaf explants of highbush blueberry. HorScience 35: 945-947.

Cao, X.; Hammerschlag, F.A. 2002. A two-step pretreatment significantly enhances shoot organogenesis from leaf explants of highbush blueberry cv. Bluecrop. HortScience 37: 819-821.

Cao, X.; Liu, Q.; Rowland, L.J.; Hammerschlag, F.A. 1998. GUS expression in blueberry (Vaccinium spp.): factors influencing Agrobacterium-mediated gene transfer efficiency. Plant Cell Reports 18: 266-270.

Castro, D.; González, J. 2002. Micropropagación de eucalipto (Eucalyptus grandis Hill ex Maiden) en el sistema de inmersión temporal. Agricultura Técnica 62(1): 68-78.

Chen, C. 2004. Humidity in plant tissue culture vessels. Biosystems Engineering 88(2): 231-241.

Cohen, D.; Elliot, D. 1979. Micropropagation methods for blueberries and tamarillos. Combined Proceedings, International Plant Propagators’ Society 29: 177-179.

Debnath, SC.; McRae, K.B. 2005. A one-step in vitro cloning procedure for cranberry (Vaccinium macrocarpon Ait.): the influence of cytokinins on shoot proliferation and rooting. Small Fruits Review 4: 57-75.

Debnath, S.C. 2005a. A two-step procedure for adventitious shoot regeneration from in vitro derived lingonberry leaves: shoot induction with TDZ and shoot elongation using zeatin. HortScience 40: 189-192.

Debnath, S.C. 2005b. Micropropagation of lingonberry: influence of genotype, explant, orientation, and overcoming TDZ induced inhibition of shoot elongation using zeatin. HortScience 40: 185-188.

Fernández-Lizarazo, J.; Mosquera-Vásquez, T. 2012. Efficient micropropagation of french tarragon (Artemisia dracunculus L.). Agronomía Colombiana 30(3): 335-344.

Fira, A.; Clapa, D.; Badescu, C. 2008. Aspects regarding the in vitro propagation of highbush blueberry cultivar blue crop. Bulletin UASVM, Horticulture 65(1):104-109.

Gajdošová, A.; Ostrolucká, M.; Libiaková, G.; Ondrušková, E.; Šimala, D. 2006. Microclonal propagation of Vaccinium sp. and Rubus sp. and detection of genetic variability in culture in vitro. Journal of Fruit and Ornamental Plant Research 14: 103-119.

González, M.; Lopez, M.; Valdes, A.; Ordas, R. 2000. Micropropagation of three berry fruit species using nodal segments from field grown plants. Annals Applied Biology 137: 73-78.

Hamad, A.M.; Taha, R.M. 2009. Effect of explants density on the in vitro proliferation and growth of separated and cluster shoots of smooth cayenne pineapple (Ananas comosus L. Merr.). Asian Journal of Plant Sciences 8: 313-317.

Hazarika, B. 2006. Morpho-physiological disorders in in vitro culture of plants. Scientia Horticulturae 108: 105-120.

Huda, K.; Bhuiyan, M.; Zeba, N.; Banu, S.; Mahmud, F.; Khatun, A. 2009. Effect of FeSO4 and pH on shoot regeneration from the cotyledonary explants of Tossa Jute. Plant Omics Journal 2(5): 190-196.

Isutsa, D.; Pritts, M.; Mudge, K. 1994. Rapid propagation of blueberry plants using ex vitro rooting and controlled acclimatization of micropropagules. HortScience 29: 1124-1126.

Kozai, T.; Jeong, B.; Kubota, C.H.; Murai, Y. 1995. Effects of volume and initial strength of medium on the growth, photosynthesis and ion uptake of potato (Solanum tuberosum) plantlets in vitro. Journal of the Japanese Society for Horticultural Science 64(1): 63-71.

Litwinczuk, W.; Szczerba, G.; Wrona D. 2005. Field performance of highbush blueberries (Vaccinium corymbosum L.) cv. 'Herbert' propagated by cuttings and tissue culture. Scientia Horticulturae 106(2): 162-169.

Lloyd, G.; McCown, B. 1980. Commercially feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture. Proceedings of the International Plant Propagators' Society 30: 421-427.

Lorenzo, J.; González, B.; Escalona, M.; Teisson, C.; Espinosa, P.; Borroto, C. 1998. Sugarcane shoots formation in an improved temporary immersion system. Plant Cell Tissue and OrganCulture 54: 197-200.

Mazri, M. 2013. Effect of basal medium, explants size and density on the in vitro proliferation and growth of date palm (Phoenix dactylifera L.) cultivar ‘16-bis’. Notulae Scientia Biologicae 5(3): 332-337.

Meiners, M.; Schwab, M.; Szankowski, I. 2007. Efficient in vitro regeneration systems for Vaccinium species. Plant Cell Tissue and Organ Culture 89:169-176.

Orellana, P. 1998. Propagación vía organogénesis. In: Pérez, J.N. (ed.). Propagación y mejora genética de plantas por biotecnología. IBP. Santa Clara. pp. 151-176.

Ostrolucká, M.G.; Libiaková, G.; Ondrušková, E.; Gajdošová, A. 2004. In vitro propagation of Vaccinium species. Acta Universitatis Latviensis, Biology 676: 207-212.

Pereira, F.D.; Pinto, J.E.; Rodríguez, H.C.; Rosado, L.D.; Beijo, L.A.; Lameira, O.A. 2006. Proliferaão in vitro de brotos de curauá utilizando diferentes volumes de meio. Plant Cell Culture and Micropropagation 2(2): 53-106.

Reed, B.M.; Abdelnour, A. 1991. The use of zeatin to initiate in vitro cultures of Vaccinium species and cultivars. HortScience 26: 1320-1322.

Reis, E.S.; Pinto, J.E.; Rosado, L.D.; Correa, R.M. 2007. Tipos de explantes e volumes de meio de cultura no cultivo in vitro de Melissa officinalis L. Plant Cell Culture and Micropropagation 3(2): 83-88.

Shende, S.; M. Rai. 2005. Multiple shoot formation and plant regeneration of a commercially-useful tropical plant, Buchanania lanzan (Spreng). Plant Biotechnology 22(1): 59-61.

SPSS. 2006. SPSS 15.0 Brief Guide. SPSS Inc. USA. 179 p.

Williams, R. 1995. The chemical microenvironment. In: Aitke, J. (ed.). Automation and environmental control in plant tissue culture. Kluwer Academic Publishers. Dordrecht, The Netherlands. pp. 405-439.

Wolfe, DE.; Eck, P.; Chee-kok, C. 1983. Evaluation of seven media for micropropagation of highbush blueberry. HortScience 18: 703-705.

Wolfe, D.; Chin, C.K.; Eck, P. 1986. Relationship of the pH of medium to growth of “Bluecrop” highbush blueberry in vitro. HortScience 21: 296-298.

Zimmerman, RH.; Broome, O.C. 1980. Blueberry micropropagation. In: Proceedings of the Conference on Nursery Production of Fruit Plants through Tissue Culture USDA-SEA, Agr. Res. Results ARR-NE. 11: 44-47.

Zimmerman, R.H. 1995. Environmental effects and their control in plant tissue culture - review. ActaHorticulturae 393: 11-14.

Zobayed, S.; Armstrong, J.; Armstrong, W. 2001. Micropropagation of potato: evaluation of closed, diffusive and forced ventilation on growth and tuberization. Annals of Botany 87: 53-59.

Received: 17/11/14; Accepted: 25/01/15

Corresponding author: E-mail: marodrig@uct.cl (M. Rodríguez).

Published

2015-03-31

How to Cite

Rodríguez Beraud, M., & Morales Ulloa, D. (2015). Effect of explant density and volume of cultivation medium on in-vitro multiplication of blueberry (Vaccinium corymbosum L.) varieties "Brigitta" and "Legacy". Scientia Agropecuaria, 6(1), 31-40. https://doi.org/10.17268/sci.agropecu.2015.01.03

Issue

Section

Original Articles