Caracterización morfológica, molecular y patogenicidad de aislados de Rhizoctonia causantes de la mancha bandeada de la vaina del maíz en el norte de Sinaloa

Karen  Rabago-Zavala; Fernando A. Valenzuela-Escoboza; Blanca E. López-Valenzuela; Quintín A. Ayala-Armenta; Glenda Judith Lizárraga-Sánchez

doi:10.17268/sci.agropecu.2025.021

Authors

Karen Rabago-Zavala Colegio de Ciencias Agropecuarias, Facultad de Agricultura del Valle del Fuerte, Universidad Autónoma de Sinaloa, Sinaloa, México. https://orcid.org/0009-0004-1566-8705
Fernando A. Valenzuela-Escoboza Colegio de Ciencias Agropecuarias, Facultad de Agricultura del Valle del Fuerte, Universidad Autónoma de Sinaloa, Sinaloa, México. https://orcid.org/0000-0001-6203-1695
Blanca E. López-Valenzuela Colegio de Ciencias Agropecuarias, Facultad de Agricultura del Valle del Fuerte, Universidad Autónoma de Sinaloa, Sinaloa, México. https://orcid.org/0000-0001-6383-6145
Quintín A. Ayala-Armenta Colegio de Ciencias Agropecuarias, Facultad de Agricultura del Valle del Fuerte, Universidad Autónoma de Sinaloa, Sinaloa, México. https://orcid.org/0000-0002-8083-4261
Glenda Judith Lizárraga-Sánchez Universidad Autónoma de Occidente, Unidad Regional Los Mochis, Departamento de Ciencias Naturales y Exactas, Sinaloa, México. https://orcid.org/0000-0001-9816-9290

DOI:

https://doi.org/10.17268/sci.agropecu.2025.021

Keywords:

anastomosis, RPB2, severity, sclerotia, evolutionary tree analysis, cladistic analysis

Abstract

Corn production is affected by fungi of the genus Rhizoctonia. The objective of this study was to characterize, using morphology, DNA sequence analysis, and pathogenicity, Rhizoctonia isolates associated with corn with banded leaf spot symptoms in commercial fields in northern Sinaloa. Diseased plants were collected in the municipalities of Ahome, El Fuerte, and Guasave, obtaining 27 isolates. Morphological characterization on PDA showed colonies with whitish to brown mycelium, pigment release into the medium, and amorphous, red and brown sclerotia with a diameter < 1 mm, characteristics described for Rhizoctonia. For molecular characterization, genomic DNA was extracted, part of the RPB2 gene was amplified by PCR, and the amplified products were sequenced. Phylogenetic analysis confirmed the identification of 18 isolates as Rhizoctonia zeae and 9 isolates as Rhizoctonia solani. The R. solani isolates correspond to the anastomosis group (AG): AG-4. Three of them correspond to the anastomosis subgroup AG-4 HGI and the other 6 to the subgroup AG-4 HGIII. The pathogenicity and severity of the isolates were evaluated in corn plants 34 days after planting, revealing significant differences (p > 0.05). This study will allow the development of disease management strategies and future research into biological and chemical control of Rhizoctonia species.

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