Evaluation of probiotic potential of lactic acid bacteria to reduce in vitro cholesterol
DOI:
https://doi.org/10.17268/sci.agropecu.2012.01.06Keywords:
Lactobacillus, cardiovascular disease, enzyme bile salt hydrolase, cholesterol reduction.Abstract
Daily consumption of probiotics reduce levels of serum cholesterol by up to 3%, which is significant to prevent hypercholesterolemia, a risk factor for cardiovascular disease and cause of mortality. The genus Lactobacillus is used in industry as a probiotic and some species reduce serum cholesterol by two mechanisms, the adsorption of cholesterol and the production of the enzyme bile salt hydrolase, which vary according to species. The aim of the study was to assess the ability of probiotic bacteria group isolated from coast serum. 53 strains were isolated from nine coastal serum sample; the sensitivity to cefoxitin and vancomycin, and the tolerance to pH 2.0 and 0.3% bile salts were evaluated to determine its probiotic potential. Five microorganisms were selected and molecularly identified as Lactobacillus fermentum. The ability to absorb cholesterol measured by the method of Kimoto, showed a reduction of 53.06 ± 2.69 μg.mL-1 for strain K73 and 7.23 ± 2.69 μg. mL-1 for K75. These same strains showed the highest total and specific activity of the enzyme. The results didn´t show a relationship between the production of enzyme and adsorption of cholesterol. The strain with the greatest probiotic potential was K73. This hypocholesterolemic property will give strains added value to start the search for food matrices that allow decreasing serum cholesterol levels.
References
Bao, Y; Zhang, Y; Liu, Y; Wang, S; Dong, X; Wang, Y; Zhang, H. 2010. Screening of potential probiotic properties of Lactobacillus fermentum isolated from traditional dairy products. Food Control 21(5): 695-701.
Belviso, S.; Giordano, M.; Dolci, P.; Zeppa, G. 2009. In vitro cholesterol-lowering activity of Lactobacillus plantarum and Lactobacillus paracasei strains isolated from the Italian Castelmagno PDO cheese. Dairy Science Technology 89(2): 169-176.
Cueto, C.; García, D.: Garcés, F.; Cruz, J. 2007. Preliminary studies on the microbiological characterization of lactic acid bacteria in Suero costeño, a Colombian traditional fermented milk product. Revista latinoamericana de microbiología 49: 11-17.
Elkins, C. A.; Moser, S. A.; Savage, D.C. 2001. Genes encoding bile salt hydrolases and conjugated bile salt transporters in Lactobacillus johnsonii 100-100 and other Lactobacillus species. Microbiology 147(12): 3403-3412.
Gardiner, G. E.; Heinemann, C.; Bruce, A. W.; Beuerman, D.; Reid, G. 2002. Persistence of Lactobacillus fermentum RC-14 and Lactobacillus rhamnosus GR-1 but not L. rhamnosus GG in the human vagina as demonstrated by randomly amplified polymorphic DNA. Clinical and Vaccine Immunology 9(1): 92-96.
Guo, X. H.; Kim, J. M.; Nam, H. M.; Park, S.Y. 2010. Screening lactic acid bacteria from swine origins for multistrain probiotics based on in vitro functional properties. Anaerobe 16(4): 321-326.
Huang, X.; Auinger, P.; Eberly, S.; Oakes, D.; Schwarzschild, M.; Ascherio, A.; Investigators, P. S. G. D. 2011. Serum cholesterol and the progression of Parkinson's disease: results from DATATOP. PLoS One, 6(8), e22854.
Hummel, A.; Hertel, C.; Holzapfel, W.; Franz, C. 2007. Antibiotic Resistances of Starter and Probiotic Strains of Lactic Acid Bacteria. Applied and Environmental Microbiology 73 (3): 730–739.
Jeun, J.; Kim, S.; Cho, S.Y.; Jun, H.; Park, H.J.; Seo, J.G.; Lee, S.J. 2010. Hypocholesterolemic effects of Lactobacillus plantarum KCTC3928 by increased bile acid excretion in C57BL/6 mice. Nutrition 26(3): 321-330.
Kimoto, H.; Ohmomo, S.; Okamoto, T. 2002. Cholesterol removal from media by lactococci. Journal of dairy science 85(12): 3182-3188.
Liong, M.T.; Shah, N.P. 2005. Bile salt deconjugation ability, bile salt hydrolase activity and cholesterol co-precipitation ability of lactobacilli strains. International Dairy Journal 15(4): 391-398.
Lye, H.S.; Rusul, G.; Liong, M.T. 2010. Removal of cholesterol by lactobacilli via incorporation and conversion to coprostanol. Journal of Dairy Science 93(4): 1383-1392.
Ministerio de la Protección Social. República de Colombia 2007. Situación de Salud en Colombia Indicadores Básicos. Disponible en: http://www. minproteccionsocial.gov.co/VBeContent/NewsDetail.asp?ID=15895&IDCompany=3.
Mori, K.; Yamazaki, K.; Ishiyama, T.; Katsumata, M.; Kobayashi, K.; Kawai, Y.; Shinano, H. 1997. Comparative sequence analyses of the genes coding for 16S rRNA of Lactobacillus casei-related taxa. Int J Syst Bacteriol 47(1): 54-57.
Pereira, D.I.A.; McCartney, A.L.; Gibson, G.R. 2003. An in vitro study of the probiotic potential of a bile-salt-hydrolyzing Lactobacillus fermentum strain, and determination of its cholesterol-lowering properties. Applied and environmental microbiology 69(8): 4743-4752.
Ray, A.; Pultz, N.; Bhalla, A.; Aron, D.; Donskey, C. 2003. Coexistence of vancomycin-resistant enterococci and Staphylococcus aureus in the intestinal tracts of hospitalized patients. Clin Infect Dis. 37(7): 875-881.
Roth, G. F; Mokdad S.; Aekplakorn A.; Hasegawa W.; Lim T. 2011. High total serum cholesterol, medication coverage and therapeutic control: an analysis of national health examination survey data from eight countries. Bulletin of the World Health Organization 89: 92-101.
Urbanska, A.M., Chen, H., Prakash, S. 2007. Investigation of microencapsulated BSH active Lactobacillus in the simulated human GI tract. Journal of Biomedicine and Biotechnology, 13684.
Vizoso-Pinto, M.G.; Franz, C.M.A.P.; Schillinger, U.; Holzapfel, W.H. 2006. Lactobacillus spp. with in vitro probiotic properties from human faeces and traditional fermented products. International journal of food microbiology 109(3): 205-214.
* Autor para correspondencia.
E-mail: maria.cueto@unisabana.edu.co (C. Cueto).
Recibido 26 noviembre 2011.
Aceptado 20 febrero 2012.
Downloads
Published
How to Cite
Issue
Section
License
The authors who publish in this journal accept the following conditions:
a. The authors retain the copyright and assign to the magazine the right of the first publication, with the work registered with the Creative Commons attribution license, which allows third parties to use the published information whenever they mention the authorship of the work and the First publication in this journal.
b. Authors may make other independent and additional contractual arrangements for non-exclusive distribution of the version of the article published in this journal (eg, include it in an institutional repository or publish it in a book) as long as it clearly indicates that the work Was first published in this journal.
c. Authors are encouraged to publish their work on the Internet (for example, on institutional or personal pages) before and during the review and publication process, as it can lead to productive exchanges and a greater and faster dissemination of work Published (see The Effect of Open Access).
