Relationship among electrical conductivity, pH of soaked-water, seed germination and seedling growth of corn (Zea mays L.) under two experimental conditions
DOI:
https://doi.org/10.17268/sci.agropecu.2011.04.03Keywords:
Seed quality, stress vigor tests, seedling growth.Abstract
Corn (Zea mayz L.) seeds of twelve lots of different acquisition years were used to study the relation among the measurements of electrical conductivity (EC) and pH of the soaking water and the germination in laboratory, under normal conditions (NC) and accelerated aging (AA) of seeds. A randomized complete block design was used with four replications, an analysis of variance was applied and differences among seed lots were detected by Least Significant Difference Test. Seed lots showed high percentage of germination at eight days of evaluation in (NC) and the unfavorable effect of AA was demonstrated. Dry shoot and radicle weights showed significant differences among the lots. Values of EC were related to percentage of germination in laboratory under AA. Seed lots that showed high percentage of germination in laboratory, previous AA, presented low values of electrical conductivity, therefore, this test could be good indicative of the behavior of corn seeds under extreme or unfavorable conditions of germination. The test of pH did not show direct relation with the seed emergency in laboratory.
References
Alves, E.; Cavariani, C.; Rocha Corrêa, M.: Gonçalves Souza, F. L.; Mattosinho Corrêa, T.; Nakagawa, J. 2004. Efeito dos períodos de envelhecimento na lixiviação de íons e de proteínas solúveis em sementes de milho. Revista Brasileira de Sementes 26 (2): 119-125.
Amaral, A.; Peske, E. S. T. 1984. pH do exudato para estimar, em 30 minutos, a viabilidade de sementes de soja. Revista Brasileira de Sementes 06 (3): 85-92.
Arango Perearnau, M. R.; Gallo, C.; Craviotto, R. M. 2006. Evaluación de calidad de semillas de soja de diferentes calibres. In III Congreso de Soja del MERCOSUR. 27 al 30 de junio de 2006, Rosario, Argentina. Sección Mecanización y Postcosecha. p 150-153.
Basra, S. M. A.; Ashraf, M.; Iqbal, N.; Khaliq, A.; Ahmad, R. 2004. Physiological and biochemical aspects of pre-sowing heat stress on cottonseed. Seed Sci. and Technol. 32: 837-846.
Besnier, F. 1989. Semillas: Biología y Tecnología. Ediciones Mundi-Prensa. Madrid. España.
Bewley, J. D. 1986. Membrane changes in seeds as related to germination and the turbatios resulting from deterioration in storage. In: Mcdonald, M. B. and Nelson, C.J. (Ed). Physiology of seed deterioration. Winsconsin: 1986. p. 27-45.
Castañeda Saucedo, M. C.; Córdova Téllez, L.; González Hernández, V. A.; Delgado Alvarado, A.; Santacruz Varela A.; García de los Santos, G. 2006. Respuestas fisiológicas, rendimiento y calidad de semilla en frijol sometido a estrés hídrico. Interciencia 31 (6): 461-466.
Changrong, Y.; Sripichitt, P.; Juntakool, S.; Hongtrakul, V.; Sripichitt, A. 2007. Modifying controlled deterioration for evaluating field weathering resistance of soybean. Kasetsart J. Nat. Sci. 41: 232-241.
Chanhan, K. P.; Purkar, J. K.; Banerjee, S. K. 1984. Ageing induced changes in seeds. Seed Research 12 (1): 56-68.
Chávez Cabrera, A.; Peske, E. S. T. 2002. Testes do pH do exsudato para sementes de milho. Revista Brasileira de Sementes 24 (1): 134-140.
Colmenares, O. 1988. Test de envejecimiento rápido en el almacenamiento de semillas. Fonaiap Divulga 6 (30): 9-11.
Copeland, L. O.; McDonlad, M. B. 2001. Principles of Seed Science and Technology. 4th edition. Kluwer Academic Publishers. U. S. A.
Cordova Tellez, L.; Burris, J. S. 2002. Embryo drying rates during the acquisition of desiccation tolerance in maize seed. Crop Science. 42: 1989-1995.
Correa-Santana, D.; Vieira, M D G. G. C.; Moreira de Carvalho, M.L. Sílva se Oliveira, E. M. 1998. Teste do pH do exsudato-fenolftaleína para rápida definição sobre o destino de lotes de sementes de milho. Revista Brasileira de Sementes 20 (1) 160-166.
Fernandes, E. J.; Sader, R.; Carvalho, E. N. M. 1987. Viabilidade de sementes de feijão (Phaseolus vulgaris L.) estimada pelo ph do exudato. Revista Brasileira de Sementes 9 (3): 69-75.
Ferreira, R. L.; de Sá, M. E. 2010. Contribuição de etapas do beneficiamento na qualidade fisiológica de sementes de dois híbridos de milho. Revista Brasileira de Sementes 32 (4): 99-110.
Fessel, S. A.; Vieira, R. D.; Pessoa da Cruz, M. C.; de Paula, R. C.; Panobianco, M. 2006. Electrical conductivity testing of corn seeds as influenced by temperature and period of storage. Pesquisa Agropecuária Brasileira 41 (10): 1551-1559.
Furbeck, S. M.; Bourland, F. M.; Watson Jr, C. E. 1993. Inheritance of resistance to seed deterioration in cotton. Euphytica 69: 203 – 209.
Garcia, L. C.; Nogueira, A. C.; Abreu, D. C. A. 2004. Influência do envelhecimento acelerado no vigor de sementes de Anadenanthera colubrina (Vellozo) Brenan – Mimosaceae. Ciência Florestal (Santa Maria) 14 (1): 85-90.
Gotardo, M.; Vieira, R. D.; Pereira, L. M. A. 2001. Teste de condutividade electrica em sementes de milho. Revista Ceres 48 (277): 333-340.
Hampton, J. G.; Johnstone, K.; Eua-Umpon, V. 1992. Bulk conductivity test variables for mungbean, soybean and French bean lots. Seed Science and Technology 20: 677-686.
Hoffman, E.; Castiglioni, E. 2006. Evaluación del efecto del insecticida Imidacloprid sobre factores asociados a la implantación y crecimiento inicial de girasol, maíz y sorgo.http://www.calister.com.uy/uploads/files/Imidacloprid___Implantacion_de_Maiz_Girasol_y_Sorgo_2.pdf
Il-Doo, J.; Joong, Y. S.; Yuko, M.; Mari, I. I. 2005. Changes in the water content and germination rate during seed desiccation and their inter-specific differences among Zizania species. Journal of the Faculty of Agriculture, Kyushu University 50 (2): 573-583.
International Rules for Seed Testing (ISTA). 1993 International rules for seed testing. Rules 1993. Seed Science and Technology 21 supl.: 1-301.
Jianhua, Z.; McDonald, M. B. 1996. The Saturated salt accelerated aging test for small-seeded crops. Seed Science and Technology 25: 123-131.
Kaewnaree, P.; Vichitphan, S.; Klanrit, P.; Siric, B.; Vichitphan, K. 2011. Effect of accelerated aging process on seed quality and biochemical changes in sweet pepper (Capsicum annuum Linn.) seeds. Biotechnology, 10: 175-182.
Kaewnareea, P.; Vichitphan, S.; Klanrit, P.; Siric, B.; Vichitphan, K. 2008. Electrolyte leakage and fatty acid changing association in accelerated aging sweet pepper seed. J. Biotechnol., 136: 149-149.
Kapoor, N.; Arya, A.; Siddiqui, M. A.; Kumar, H.; Amir, A. 2011. Physiological and biochemical changes during seed deterioration in aged seeds of rice (Oriza sativa L.). American Journal of Plant Physiology 6 (1): 28-35.
Lahuta, L. B.; Górecki, R. J.; Zalewski, K.; Hedley, C. L. 2007. Sorbitol accumulation during natural and accelerated ageing of pea (Pisum sativum L.) seeds. Acta Physiol Plant 29: 527–534.
Martins, C. C.; Martinelli Seneme, A.; Castro, M. M.; Nakagawa. J.; Cavariani, C. 2002. Comparação entre métodos para a avaliação do vigor de lotes de sementes de couve-brócolos (Brassica oleracea L. var.italica Plenk). Rev. Bras. Sementes 24 (2): 96-101.
Matthews, S.; Powell, A. 2006. Electrical conductivity vigour test: Physiological basis and use. Seed Testing International 131: 32-35.
McDonald, M. B. 2002. Standardization of Seed Vigor Test. p 200 – 208. In: McDonald, M. B. y S. Contreras (Ed.). Seeds: Trade, Production and Technology. Proceedings Internacional Seed Seminar. Colección de Extensión. Facultad de Agronomía e Ingeniería Forestal. Pontificia Universidad Católica de Chile. 15-16 Octubre. Santiago. Chile.
McDonald, M. B. 1998. Seed quality assessment. Seed Science Research. 8: 265-275.
Méndez-Natera, J.; Vergara, M. 2000. Deterioro de la calidad de la semilla de tres híbridos de maíz (Zea mays L.) en función de diferentes períodos de almacenamiento en cámara de envejecimiento acelerado. V Jornadas Cientificas del Maíz. Guanare, Nov. 2000. http://www.ceniap.gov.ve/pbd/Congresos/ jornadas de maiz/5 jornadas/ envejecimiento.htm.
Mohammadi, H.; Soltani, A.; Sadeghipour, H. R.; Zeinali, E. 2011. Effects of seed aging on subsequent seed reserve utilization and seedling growth in soybean. International Journal of Plant Production 5 (1): 65-70.
Mora Aguilar, R.; Quintero de la Cruz, J. A.; Martínez Solís, J.; Rodríguez Pérez, J. E. 2006. Pruebas de calidad fisiológica de semilla de cebolla y su relación con el establecimiento en almácigo. In LII Reunión Anual de la Sociedad Interamericana de Horticultura Tropical. del 24 al 30 de septiembre, Mayagüez, Puerto Rico. p. 77-78.
Neya, O. 2006. Conservation of tree seeds from tropical dry-lands. Ph. D. Thesis, Wageningen University, The Netherlands. 157 pp.
Nik, S. M. M.; Tilebeni, H. G.; Zeinali, E.; Tavassoli, A. 2011. Effects of seed ageing on heterotrophic seedling growth in cotton. American-Eurasian J. Agric. And Environ. Sci. 10(4): 653-657.
Ouyang, X.; van Voorthuysen, T:; Toorop, P. E.; Hilhorst, H. W. M. 2002. Seed vigor, aging, and osmopriming affect anion and sugar leakage during imbibition of maize (Zea mays L.) caryopses. International Journal of Plant Sciences 163(1): 107-112.
Panobianco, M.; Marcos Filho, J. 2001. Evaluation of the physiological potencial of tomato seeds by germina-tion and vigor test. Seed Technology 23(2): 151-161
Peske, S. T.; Amaral, A. 1986. Prediction of germination of soybean seeds by measurement of pH of seed exudates. Seed Science and Technology 14: 151-156.
Pontes, C.A.; Borges Corte, V.; De Lima, E.; Borges, E. E.; Gomes Da Silva, A.; De Cássia Gonçalves Borges, R. 2006. Influência da temperatura de armazenamento na qualidade das sementes de Caesalpinia peltophoroides Benth. (Sibipiruna). Árvore 30: 43-48.
Powell, A. A. 1986. Cell membranes and seed leachate conductivity in relation to the quality of seed for sowing. Journal of Seed Technology 10: 81-100.
Powell, A. A. 1988. Seed vigour and field establishment. Advances in Research and Technology of Seeds. 11: 29-61.
Powell, A. A. 2006. Conductivity testing for Phaseolus vulgaris. Seed Testing International 132: 35-36.
República de Venezuela. Gaceta Oficial. Año CXIII. Mes VII. Caracas: 25.04.1986. N° 33.456. Resolución por la cual se dictan "Normas generales sobre semillas".
Rincón, F.; Molina, J. 1990. Efecto del método de envejecimiento artificial sobre la germinación de semillas de maíz. Agronomía Mesoamericana: 51-53.
Rodrigues Maia. A.; Lopes, J. C.; de Oliveira Teixeira, C. 2007. Efeito do envelhecimento acelerado na avaliação da qualidade fisiológica de sementes de trigo. Ciênc. Agrotec. 31 (3): 678-684.
Salazar, P.; Trejo, A.; Hernández, L. M. 2006. Pruebas de envejecimiento acelerado en semillas de maíz (Zea mays L.) de diferentes bases genéticas. Rev. Unell. Cienc. Tec. 24: 63-69.
Salisbury, F. B.; Ross, C. W. 1992. Plant Physiology. 4th edition. Wadsworth Publishing Company. U.S.A.
Santos, S. R. G. dos.; de Paula, R. C. 2007. Teste de envelhecimento acelerado para avaliação do vigor de lotes de sementes de Sebastiania commersoniana (Baill.) Smith & Sowns (Branquilho) – Euphorbiaceae. Rev. Inst. Flor. (São Paulo) 19 (1): 1-12.
Shah, F.; Watson, C.; Cabrera, E. 2002. Seed vigour testing of subtropical corn hybrids. Mississippi Agricultural and Forestry Experiment Station. Research Report 23(2): 6pp.
Silva, N. O. 2006. Controle genético de características associadas à qualidade de sementes de milho. Tese Doutorado. Universidade Federal de Lavras. Lavras, Minas Gerais, Brasil. 92 pp.
Singh, B.; Singh, C. B.; Gupta, P. C. 2003. Influence of seed ageing in Vigna species. Farm Science Journal 12 (1): 4-7.
Taiz, L.; Zeiger, E. 2002. Plant physiology. 3th Edition. Sinauer Associates Inc. Publishers. Sunderland. U.S.A.
Tajbakhsh, M. 2000. Relationships between electrical conductivity of imbibed seeds leachate and subsequent seedling growth (Viability and vigour) in Omid wheat. J. Agr. Set. Tech., 2: 67-71.
Villarroel, N.; Méndez-Natera, J. 2007. Efecto del envejecimiento acelerado sobre el crecimiento de plántulas de nueve lotes de semillas de diferentes cultivares de maíz (Zea mays L.). LVII Convención Anual de la AsoVAC. Acta Científica Venezolana Volumen 58 (Sup. 1): 38. (Documento en CD-ROM).
Viloria, H.; Méndez Natera, J. 2007. Determinación del período de remojo adecuado para las mediciones de pH y de conductividad eléctrica en semillas de maíz (Zea mays). Boletín del Centro de Investigaciones Biológicas 41 (2): 227–243.
Wagner M. H.; Préveaux, A.; Moizan, E.; Ducournau, S. 2004. Vigour testing: towards an extended use of the conductivity test. 27th I.S.T.A. Seed Symposium, Budapest, Hungary, 17–19 May. p. 16.
Wagner, M. H.; Ducournau, S. 2007. Conductivity testing for oilseed rape seeds. Seed Testing International 133: 40-41.
Wanli, Z.; Leihong, L. I.; Gultieri de Andrade Perez, S. C. J. 2001. Pré-condicionamento e seus efeitos em sementes de canafístula (Peltophorum dubium (Spreng.) Taub.). Revista Brasileira de Sementes 23 (1): 146-153.
Welbaum, G. E.; Bradford, K. J. 1990. Water relations of seed development and germination in muskmelon (Cucumis melo L.). IV. Characteristics of the perisperm during seed development. Plant Physiol. 92: 1038-1045.
Wilson, J. R. D. 1992. A unified approach to interpretation of single seed conductivity data. Seed Science and Technology 20: 155-163.
Woodstock, L.W., Feeley, J. 1965. Early seedling growth and initial respiration 7 rates as potential indicators of seed vigour in corn. Proc. Int. Seed Test. Ass., 55: 131-139.
* Autor para correspondencia.
E-mail: jmendezn@cantv.net (V. Vásquez).
Recibido 10 agosto 2011.
Aceptado 19 diciembre 2011.
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