Identification of the 5s rDNA locus in Physalis peruviana L. chromosomes, first report

Authors

  • María Siles-Vallejos Grupo de Investigación en Recursos Genéticos (RecGen), Facultad de Ciencias Biológicas, UNMSM. Lima
  • Olga Bracamonte-Guevara Grupo de Investigación Citogenética y Organismos Modelo (CISMOD), Facultad de Ciencias Biológicas, UNMSM, Lima
  • Marlon García-Paitán Grupo de Investigación en Recursos Genéticos (RecGen), Facultad de Ciencias Biológicas, UNMSM. Lima
  • Alberto López-Sotomayor Grupo de Investigación en Recursos Genéticos (RecGen), Facultad de Ciencias Biológicas, UNMSM. Lima. Peru

DOI:

https://doi.org/10.17268/sci.agropecu.2021.016

Keywords:

Golden Berry, FISH, chromosome, 5S rDNA locus, cytogenetics

Abstract

Physalis peruviana (Golden berry) a native fruit of Peru, is an important Andean agricultural crop. Although classical cytogenetics research on this berry has been conducted there is no precise evidence based on molecular cytogenetic studies. The Fluorescent In Situ Hybridization (FISH) technique was applied to reveal 5S rDNA sites in chromosomes of San Pablo and Celendín populations, Cajamarca, Perú. This research was identified the 5S rDNA locus in the two populations of Golden berry, reporting six signals in 3 pairs of chromosomes which would indicate that the species is polyploid. Likewise, no differences were found in the number of 5S rDNA loci between both populations, so we could point out that they would not be different ecotypes.

References

Aguilera, P. M., Debat, H. J., Scaldaferro, M. A., et al. (2016). FISH-mapping of the 5s rDNA locus in chili peppers (Capsicum-Solanaceae). Anais Da Academia Brasileira de Ciencias, 88(1), 117–125.

Aliyeva-Schnorr, L., Ma, L., & Houben, A. (2015). A fast air-dry dropping chromosome preparation method suitable for FISH in plants. Journal of visualized experiments, 106, e53470.

Bonilla, H., Carbajal, Y., Siles, M., & López, A. (2019). Diversidad genética de tres poblaciones de Physalis peruviana a partir del fraccionamiento y patrón electroforético de proteínas de reserva seminal. Revista Peruana de Biología, 26(2), 243–250.

Carbajal, Y. N. (2018). Caracterización citogenética de tres ecotipos de Physalis peruviana “Aguaymanto” provenientes del departamento de Cajamarca: Diversidad y evolución. Tesis para optar el Título Profesional de Bióloga Biotecnóloga. Universidad Nacional Mayor de San Marcos. Lima. Perú. 155 p.

Chasquibol, N., & Yácono, J.C. (2015). Composición Fitoquímica del aceite de las semillas del fruto del "Aguaymanto", Physlais peruviana L. Revista de la Sociedad Química del Perú. Revista de la Sociedad Química del Perú, 81(4), 311–318.

De Melo, N. F., & Guerra, M. (2003). Variability of the 5S and 45S rDNA sites in Passiflora L. species with distinct base chromosome numbers. Annals of Botany, 92(2), 309–316.

De Paula, A. A., Fernandes, T., Vignoli-Silva, M., & Vanzela, A. L. L. (2015). Comparative cytogenetic analysis of Cestrum (Solanaceae) reveals different trends in heterochromatin and rDNA sites distribution. Plant Biosystems, 149(6), 976–983.

Deza, J., & Delgado, F. (2017). La domesticación de los Andes. Universidad Alas Peruanas. 978-612-4357-06-0. Perú. 206 p.

Dostert, N., Roque, J., Brokamp, G., et al. (2013). Seven vascular plants species used in Peru: Factsheet botanical. Arnaldoa, 20(2), 359-432.

Doyle, J. J., & Doyle, J. L. (1990). Isolation of DNA from small amounts of plant tissues. Focus, 12, 13-15.

Escobar, S.A. (2016). Determinación de cariotipo y nivel de ploidia de aguaymanto Physalis peruviana L. Tesis para optar el Título Profesional de Ingeniero Agrónomo. Universidad Nacional del Centro del Perú. 89 p.

Fukushima, K., Imamura, K., Nagano, K., & Hoshi, Y. (2011). Contrasting patterns of the 5S and 45S rDNA evolutions in the Byblis liniflora complex (Byblidaceae). Journal of Plant Research, 124(2), 231–244.

Garcia, S., Kovařík, A., Leitch, A. R., & Garnatje, T. (2017). Cytogenetic features of rRNA genes across land plants: analysis of the Plant rDNA database. The Plant Journal, 89(5), 1020–1030.

Jiang, J. (2019). Fluorescence in situ hybridization in plants: recent developments and future applications. Chromosome Research, 27(3), 153–165.

Liberato, S., Sánchez-Betancourt E., Arguelles, J. H., et al. (2014). Citogenética de genotipos de uchuva, Physalis peruviana L., y Physalis floridana Rydb. Corpoica Cienc. Tecnol. Agropecu., 15(1), 51–61.

Liu, C., Liu, J., Li, H., Zhang, Z., et al. (2010). Karyotyping in melon (Cucumis melo L.) by cross-species fosmid fluorescence in situ hybridization. Cytogenetic and Genome Research, 129(1–3), 241–249.

Moscone, E. A., Scaldaferro, M. A., Grabiele, M., et al. (2007). The evolution of chili peppers (Capsicum-solanaceae): a cytogenetic perspective. VI The International Solanaceae Conference. Acta Horticulturae, 745, 137-170

Muravenko, O.V., Amosova, A.V., Samatadze, T.E., et al. (2004). Chromosomal localization of 5S and 45S ribosomal DNA in species of Linum L. section Linum (syn=Protolinum and Adenolinum). Genetika, 40(2), 256–260.

Neves, N., Delgado, M., Silva, M., et al. (2005). Ribosomal DNA heterochromatin in plants. Cytogenetic and Genome Research, 109(1–3), 104–111.

Obregón-La Rosa, A. J., Augusto-Elías-Peñafiel, C. C., Contreras-López, et al. (2021). Características fisicoquímicas, nutricionales y morfológicas de frutas nativas. Revista de Investigaciones Altoandinas, 23(1), 17–25.

Poggio, L., Confalonieri, V., Comas, C., Gonzalez, G., & Naranjo, C.A. (2000). Evolutionary relationships in the genus Zea: Analysis of repetitive sequences used as cytological FISH and GISH markers. Genetics and Molecular Biology, 23(4), 1021–1027.

Puente, L., Pinto, M.C., Castro, E., & Cortés, M. (2011). Physalis peruviana L, the multiple properties of a highly functional fruit: A review. Food Research International, 44, 1733-1740.

Rodríguez, N., & Bueno, M. L. (2006). Estudio de la diversidad citogenética de Physalis peruviana L. (Solanaceae). Acta Biol. Colomb, 11(2), 75–85.

Setiawan, A. B., Purwantoro, A., & Wibowo, A. (2020). Cytological distinctions between timun suri and cucumber discovered by fluorescence in situ hybridization (Fish) using 45s ribosomal DNA gene. Agrivita, 42(3), 584–592.

Volkov, R., Zanke, C., Panchuk, I., Hemleben, V. (2001). Molecular evolution of 5S rDNA of Solanum species (sect. Petota): application for molecular phylogeny and breeding. Theor Appl Genet, 103, 1273–1282

Yagi, K., Pawelkowicz, M., Osipowski, P., et al. (2015). Molecular Cytogenetic Analysis of Cucumis; Wild Species Distributed in Southern Africa: Physical Mapping of 5S and 45S rDNA with DAPI. Cytogenetic and Genome Research, 146(1), 80–87.

Published

2021-04-28

How to Cite

Siles-Vallejos, M. ., Bracamonte-Guevara, O., García-Paitán, M. ., & López-Sotomayor, A. . (2021). Identification of the 5s rDNA locus in Physalis peruviana L. chromosomes, first report. Scientia Agropecuaria, 12(2), 149-153. https://doi.org/10.17268/sci.agropecu.2021.016

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