Early detection of resistance to Mycosphaerella fijiensis in local genotypes of Musa in Ecuador

Authors

  • Galo Cedeño García Escuela Superior Politécnica Agropecuaria de Manabí “Manuel Félix López”, Campus Politécnico El Limón, Calceta, Manabí.
  • Carmen Suarez Capello Facultad de Ciencias Agrarias, Universidad Técnica Estatal de Quevedo, Campus “Manuel Haz Álvarez”, Av. Quito km. 11/2 vía a Santo Domingo de los Tsáchilas, Quevedo, Los Ríos.
  • Danilo Vera Coello Departamento de Protección Vegetal, Estación Experimental Tropical Pichilingue, Instituto Nacional de Investigaciones Agropecuarias, km. 5 vías Quevedo – El Empalme, Quevedo, Los Ríos.
  • Carlo Fadda Científicos Senior de Bioversity International. Via dei Tre Denari, 472/a 00057 Maccarese, Rome.
  • Devra Jarvis Científicos Senior de Bioversity International. Via dei Tre Denari, 472/a 00057 Maccarese, Rome.
  • Paola de Santis Científicos Senior de Bioversity International. Via dei Tre Denari, 472/a 00057 Maccarese, Rome.

DOI:

https://doi.org/10.17268/sci.agropecu.2017.01.03

Keywords:

Musa cultivars, inoculation, inoculum, resistance, biodiversity

Abstract

The aim of this research was to determine resistance reaction of 14 Musa genotypes against Mycosphaerella fijensis, causal agent of Black Sigatoka disease. Two experiments were conducted in laboratory and shadehause at Pichilingue research Station from the National Institute of Agricultural Research (INIAP), Ecuador. Genotypes considered were: Orito (AA), Guineo de jardín, Williams, Filipino, Gross Mitchel, Mulato and Morado, (AAA); Limeño, Maqueño, Dominico, Dominico-Hartón, Dominico gigante, Dominico negro and Barraganete from the AAB genome. Two inoculation methods were used to asses’ resistance: a) in vitro inoculation over leaf fragments with a conidial suspension, and b) inoculation of eight weeks old seedlings with a mycelial suspension of the fungus. In the first case, the incubation period (IP) and the number of lesions at 14 and 20 days after inoculation (DDI) were measured. For in vitro trial, the incubation period (IP) and the number of leaf lesions 14 and 20 days after inoculation (DDI) were measured, and for seedlings, PI, period of symptoms evolution (TES) and time for disease development (TDE) were recorded. Statistically significant (p ≤ 0.05) differences were found for all variables. Cultivars Orito (AA) and Limeño (AAB) qualify as resistant while all the others were susceptible, though response to inoculation within the latter group showed a range of variability.

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Received October 17, 2016.

Accepted March 06, 2017.

Corresponding author: alex.musaespam@gmail.com (G. Cedeño).

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Published

2017-04-03

How to Cite

García, G. C., Capello, C. S., Coello, D. V., Fadda, C., Jarvis, D., & de Santis, P. (2017). Early detection of resistance to Mycosphaerella fijiensis in local genotypes of Musa in Ecuador. Scientia Agropecuaria, 8(1), 29-42. https://doi.org/10.17268/sci.agropecu.2017.01.03

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Vol. 8 No. 1 (2017): January - March

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Original Articles

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