Characterization symptomatology and molecular from papaya ringspot (PRSV) that infects Carica papaya L. in northern Peru
DOI:
https://doi.org/10.17268/sci.agropecu.2015.04.01Keywords:
mosaic, distortion, mechanical inoculation, plants infectedAbstract
The objective of present study was symptomatic and molecular characterization of the virus that infects Carica papaya L. in areas of northern Peru. To do this, of different fields were collected leaves of C. papaya with mosaic symptoms, chlorosis and distortion the leaf. Sap of these leaves was inoculated mechanically onto virus-free plants of C. papaya, Chenopodium murale, Ch. amaranticolor, Ch. quinoa, Cucumis melo, C. sativus and Cucurbita pepo; which they were kept at room temperature for 45 days, after which young leaves in of C. papaya, mosaic, distortion and reduction of the leaf blade was observed; in the species C. melo, C. sativus and Cucurbita pepo systemic chlorosis. Ch. murale, Ch. amaranticolor and Ch. quinoa they no showed symptoms evident. The plants infected were analyzed by serological technique NCM-ELISA and RT-PCR proving that the virus that is infecting the plantations assessed in the north of Peru, it is the papaya ringspot virus (RSVP).References
Brunt, A.; Crabtree, K.; Gibbs, A. 1990. Viruses of tropical plants. CAB International. Redwood Press Ltd. Melksham. Wiltshire. 707 p.
Cabrera, D.; Sosa, R.; Portal, O.; Alburquerque, Y.; González, J.; Hernández, R. 2009 Alterations induced by Papaya ringspot potyvirus on chlorophyll content in papaya (Carica papaya L.) leaves. Fitosanidad 13: 125-126.
Chiriboga, G. 2000. Caracterización del cultivo de papaya como producto con potencial para exportación, con énfasis en el diagnóstico molecular y serológico de enfermedades. Proyecto especial del programa de Ing. Agrónomo. El Zamorano, Honduras, 75 p.
CMI/AAB. 1994. Descriptions of plant viruses: Papaya Ringspot Virus. Surrey, England. N° 292.
Fribourg, C.E. 1984. El virus de la mancha anillada del papayo en el Perú. Fitopatología 19 (2): 46.
Gonsalves, D. 1998. Control of Papaya ringspot virus in papaya: a case study. Annual Review of Phytopathology 36: 415-437
González, A.; Trujillo, G.; Vegas, A. 2003. Uso de hospedantes diferenciales para la identificación de cepas del virus de la mancha anillada de la lechosa (PRSV). Revista Mexicana de Fitopatología 21(1): 67-70.
INIA. 2010. INIA estudia solución a arrasadora plaga en papaya.
Disponible en: http://tiempoagrario.blogspot.pe
Instituto de Investigaciones de la Amazonia Peruana (IIAP). 2010. Guía técnica del cultivo de papaya. 38p.
Kelaniyangoda, D.; Madhubashini, L. 2008. Indicator plants: Tools for detecting Papaya ring spot potyvirus and Cucumber mosaic Cucumovirus. University of Peradeniya Sri Lanka. Journal of Food and Agriculture 1(2): 64-69.
Marín, J.; Alpartida, C.; Tenorio, J. 2010. Brote reciente de la mancha anillada del papayo en el Perú. XXI congreso Peruano de Fitopatología. Tarapoto-Perú.
Marys, E.; Carballo, O.; Izaguirre, M. 2000. Ocurrence and relative incidence of viruses infecting papaya in Venezuela. Annals of Applied Biology 136: 121-124.
Ministerio de Agricultura (MINAG). 1982. Reunión de coordinación para analizar la problemática fitosanitaria del cultivo del papayo en la zona central de Perú. 2p.
Noa-Carranza, J.; Gonzales-De-Leon, D.; Silva-Rosales, L. 2007. Caracterización molecular de un aislamiento severo del virus de la mancha anular de la papaya en México y su relación con otros aislamientos. Virus Genes 35: 109-117.
Purcifull, D.; Edwarson, J.; Heibert, E.; Gonsalves, D. 1984. Papaya ringspot virus No. 292 (revised No 84). In: Descriptions of Plants Viruses. England. Kew Surrey. CMI/AAB. 8p.
Rieger, M. 2006. Introduction to Fruit Crops. Haworth Press, Binghamton, New York. 464 p.
Sánchez de Luque, C.; Martínez-López, G. 1998. El virus de la mancha anular de la papaya (Papaya Ringspot Potyvirus, PRSV) en Colombia. Universidad de Caldas, Manizales. Colombia. Virologia, Julio Nº 17.
Shuka, A.; Ward, C.; Brunt A. 1994. The potiviridae. CAB. International. Wallingford, UK. 516 p.
Tenorio, J.; Salazar, J.; Fuentes, S.; Aguilar, C. 2007. Integrated management of papaya ringspot virus (PRSV) in Peru. 10 th Plant Virus Epidemiology Symposium, ICRISAT, India.
Vegas, A.; Cermeli, M.; Trujillo, G. 1985. Importancia, distribución e incidencia de los virus que afectan a la lechosa (Carica papaya) en algunas zonas productoras del país. Agronomía Tropical (Maracay) 14: 213-237.
Recibido 13 enero 2014.
Aceptado 16 noviembre 2015.
Corresponding author: E-mail: ccedano@unitru.edu.pe (C. Cedano).
Published
How to Cite
Issue
Section
License
The authors who publish in this journal accept the following conditions:
a. The authors retain the copyright and assign to the magazine the right of the first publication, with the work registered with the Creative Commons attribution license, which allows third parties to use the published information whenever they mention the authorship of the work and the First publication in this journal.
b. Authors may make other independent and additional contractual arrangements for non-exclusive distribution of the version of the article published in this journal (eg, include it in an institutional repository or publish it in a book) as long as it clearly indicates that the work Was first published in this journal.
c. Authors are encouraged to publish their work on the Internet (for example, on institutional or personal pages) before and during the review and publication process, as it can lead to productive exchanges and a greater and faster dissemination of work Published (see The Effect of Open Access).
