Response of the transcription factor BABY BOOM of Arabidopsis thaliana L. in the formation of embryogenic calluses of cocoa leaves (Theobroma cacao L.)

Nikkolas Javier  Alburqueque-Vasquez; Angel David  Hernández-Amasifuen; Alexandra Jherina  Pineda-Lázaro; Jaime N.  Fernández Ponce; Juan Carlos  Guerrero-Abad; Ligia  García; Mike Anderson  Corazon-Guivin

doi:10.17268/sci.agropecu.2023.031

Autores/as

Nikkolas Javier Alburqueque-Vasquez Instituto de Investigación, Innovación y Desarrollo para el Sector Agrario y Agroindustrial de la Región Amazonas (IIDAA). Universidad Nacional Toribio Rodríguez de Mendoza de Amazonas, Chachapoyas
Angel David Hernández-Amasifuen Laboratorio de Biología y Genética Molecular, Universidad Nacional de San Martín
Alexandra Jherina Pineda-Lázaro Laboratorio de Biología y Genética Molecular, Universidad Nacional de San Martín
Jaime N. Fernández Ponce Departamento de Ciencias Biológicas, Universidad Nacional de Piura
Juan Carlos Guerrero-Abad Instituto de Investigación, Innovación y Desarrollo para el Sector Agrario y Agroindustrial de la Región Amazonas (IIDAA). Universidad Nacional Toribio Rodríguez de Mendoza de Amazonas, Chachapoyas
Ligia García Instituto de Investigación para el Desarrollo Sustentable de Ceja de Selva (INDES-CES), Universidad Nacional Toribio Rodríguez de Mendoza de Amazonas
Mike Anderson Corazon-Guivin Laboratorio de Biología y Genética Molecular, Universidad Nacional de San Martín

DOI:

https://doi.org/10.17268/sci.agropecu.2023.031

Palabras clave:

Embryogenic calluses, Arabidopsis thaliana, Theobroma cacao, BABY BOOM, transcription factor

Resumen

Cocoa (Theobroma cacao L.) is one of the most important economic crops worldwide. The propagation of elite varieties of cocoa has been achieved through somatic embryogenesis, but still one of the main limitations is the low rates of embryo formation, which is a genotype-dependent trait. Manipulation of transcription factors (TFs) such as BABY BOOM (BBM) promotes the transition of cocoa somatic cells from the vegetative to the embryonic state. This work validated the use of clonal cocoa leaves cv. IMC-67 to induce somatic embryogenesis, overcoming their recalcitrant limitation with the help of the introduction of TF-BBM from Arabidopsis thaliana (AtBBM). The vectors were constructed by the Gateway system using the donor vector pENTR/D-TOPO and the expression vector pk7WG2. The overexpression vector pk7WG2:AtBBM was obtained, allowing successful transformation into Agrobacterium tumefaciens GV3101. The AtBBM gene was characterized (1755 base pairs), and its expression was observed in the formation of embryogenic calluses in cocoa leaves. Overexpression of AtBBM allowed the obtainment of a 92% response in the formation of embryogenic callus in cocoa leaves with Agrobacterium-mediated vacuum infiltration and overexpression of the pk7WG2:AtBBM vector. This high transformation efficiency reached with the insertion of the overexpression vector provides validation of transient response of the TF AtBBM in the formation of embryogenic calluses in cocoa leaves of the IMC-67 clone. Through this methodology, it is possible to continue with studies of gene overexpression, insertion, silencing, and gene editing in Peruvian cocoa.

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