EFECTO ANTIOXIDANTE DEL EXTRACTO HIDROALCOHÓLICO DE Annona muricata L. FRENTE A ESTRÉS OXIDATIVO INDUCIDO POR ACRILAMIDA EN HÍGADO DE RATÓN

Cinthya Aspajo Villalaz; Sarah Pajuelo Mendoza; Jesús Morales Aniceto; Abhel Calderón Peña

Authors

Cinthya Aspajo Villalaz Universidad Nacional de Trujillo
Sarah Pajuelo Mendoza Universidad Nacional de Trujillo
Jesús Morales Aniceto Universidad Nacional de Trujillo
Abhel Calderón Peña Universidad Nacional de Trujillo

Abstract

Annona muricata posee compuestos bioactivos con propiedades antioxidantes. El objetivo de esta investigación fue determinar el efecto antioxidante del extracto hidroalcohólico de A. muricata frente al estrés oxidativo inducido por acrilamida en hígado de ratón. Se obtuvo el extracto hidroalcohólico de hojas de A. muricata; se indujo estrés oxidativo usando acrilamida vía intraperitoneal; se formaron cinco grupos: tres experimentales GE10, GE30 y GE50 (tratados con extracto a dosis de 10, 30 y 50 mg/Kg pc, respectivamente), un grupo control negativo (CN) sin inducción de estrés oxidativo, un grupo control positivo (CP) con inducción de estrés oxidativo, ambos tratados con solución salina fisiológica. El estrés oxidativo se determinó mediante la técnica de reacción de sustancias al ácido tiobarbitúrico; a los resultados se aplicaron las pruebas de normalidad de Shapiro-Wilk, análisis de varianza (ANAVA) y Tukey, con un 95% de confianza. La prueba de Shapiro-Wilk mostró una tendencia normal de cada grupo; la prueba ANAVA mostró un p<0,05; en la prueba Tukey se formaron 3 grupos homogéneos: a (CN=3,52±0,27 µg malondialdehído/g de hígado (µgMDA/gH) y GE30=3,22±0,39 µgMDA/gH), b (CP=4,87±0,20 µgMDA/gH y GE10=4,56±0,28 µgMDA/gH) y c (GE50=2,37±0,25 µgMDA/gH). Se determinó que existe efecto antioxidante del extracto hidroalcohólico de A. muricata L. frente a estrés oxidativo inducido por acrilamida en hígado de ratón, siendo la dosis de 50 mg/Kg pc la que mostró el mayor efecto antioxidante, la cual generó una concentración de 2,37 µgMDA/g de tejido hepático.

Palabras claves: Annona muricata, estrés oxidativo, antioxidantes

ABSTRACT

Annona muricata has bioactive compounds with antioxidant properties. The objective of this research was to determine the antioxidant effect of the hydroalcoholic extract of A. muricata against the oxidative stress induced by acrylamide in mouse liver. The hydroalcoholic extract of A. muricata leaves was obtained; oxidative stress was induced using acrylamide intraperitoneally; five groups were formed: three experimental GE10, GE30 and GE50 (treated with extract at doses of 10, 30 and 50 mg/Kg bw, respectively), a negative control group (CN) without induction of oxidative stress, a positive control group (PC) with induction oxidative stress, both treated with physiological saline. Oxidative stress was determined using the technique of reaction of substances to thiobarbituric acid; Shapiro-Wilk normality tests, analysis of variance (ANOVA) and Tukey were applied to the results, with 95% confidence. The Shapiro-Wilk test showed a normal trend for each group; the ANAVA test showed a p <0.05; In the Tukey test, 3 homogeneous groups were formed: a (CN = 3.52 ± 0.27 µg malondialdehyde/g liver (µgMDA/gH) and GE30 = 3.22 ± 0.39 µgMDA/gH), b (CP = 4.87 ± 0.20 µgMDA/gH and GE10 = 4.56 ± 0.28 µgMDA/gH) and c (GE50 = 2.37 ± 0.25 µgMDA/gH). It was determined that there is an antioxidant effect of the hydroalcoholic extract of A. muricata L. against the oxidative stress induced by acrylamide in mouse liver, being the dose of 50 mg / Kg bw the one that showed the highest antioxidant effect, which generated a concentration of 2.37 µgMDA / g of liver tissue.

Keywords: Annona muricata, oxidative stress, antioxidants

DOI: http://dx.doi.org/10.17268/rebiol.2020.40.02.01

References

Adefegha, S., Oyeleye, S., y Oboh, G. (2015). Distribution of phenolic contents, antidiabetic potentials, antihypertensive properties, and antioxidative effects of soursop (Annona muricata L.) fruit parts in vitro. Biochemistry research international, 2015, 347673. https://doi.org/10.1155/2015/347673.

Adewole, S., y Ojewole, J. (2008). Protective effects of Annona muricata Linn. (Annonaceae) leaf aqueous extract on serum lipid profiles and oxidative stress in hepatocytes of streptozotocin-treated diabetic rats. African journal of traditional, complementary, and alternative medicines: AJTCAM, 6(1), 30-41. https://doi.org/10.4314/ajtcam.v6i1.57071.

Agu, K., y Okolie, P. (2017). Proximate composition, phytochemical analysis, and in vitro antioxidant potentials of extracts of Annona muricata (Soursop). Food Science & Nutrition, 5(5), 1029-1036. doi:10.1002/fsn3.498.

Ansar, S., Siddiqi, N., Zargar, S., Ganaie, M., y Abudawood, M. (2016). Hepatoprotective effect of Quercetin supplementation against Acrylamide-induced DNA damage in wistar rats. BMC complementary and alternative medicine, 16(1), 327. https://doi.org/10.1186/s12906-016-1322-7.

Arts, J., Kramer, K., Arndt, S., y Ohl, F. (2014). Sex Differences in Physiological Acclimatization after Transfer in Wistar Rats. Animals: an open access journal from MDPI, 4(4), 693-711. https://doi.org/10.3390/ani4040693.

Barrios, E., Espinoza, M., Leal, U., Ruiz, N., Pinto, V., y Jurado, B. (2011). Bioética y el empleo de animales de experimentación en investigación. Salus, 15(2), 28-34.

Beland, F., Olson, G., Mendoza, M., Marques, M., y Doerge, D. (2015). Carcinogenicity of glycidamide in B6C3F1 mice and F344/N rats from a two-year drinking water exposure. Food and chemical toxicology: an international journal published for the British Industrial Biological Research Association, 86, 104-115. https://doi.org/10.1016/j.fct.2015.09.017.

Benatti, A., Carnevalli, N., Rodrigues, R., Machado, M., da Silva, N., y Salmen, F. (2018). Annona Muricata Linn. Leaf as a source of antioxidant compounds with in vitro antidiabetic and inhibitory potential against α-amylase, α-glucosidase, lipase, non-enzymatic glycation and lipid peroxidation. Biomedicine y Pharmacotherapy, 100, 83-92. doi: 10.1016/j.biopha.2018.01.172.

Bradford B, Kono H, Isayama F, Kosyk O, Wheeler M, Akiyama T, Bleye L, Krausz K, Gonzalez F, Koop D, y Rusyn I. (2005). Cytochrome P450 CYP2E1, but not nicotinamide adenine dinucleotide phosphate oxidase, is required for ethanol-induced oxidative DNA damage in rodent liver. Hepatology, 41(2): 336–344. doi:10.1002/hep.20532.

Contesse B. (2010). Evaluación de la lipoperoxidación in vitro, a través de las reacciones del 3-metil-2-benzotiazolidon hidrazona (MBTH) y del ácido tiobarbitúrico (TBARS). Tesis de titulación. Universidad de Chile.

Coria-Tellez, A., Montalvo-Gonzalez, E., Yahia, E., y Obledo-Vazquez, E. (2016). Annona muricata: A comprehensive review on its traditional medicinal uses, phytochemicals, pharmacological activities, mechanisms of action and toxicity. Arabian Journal of Chemistry, 11(5): 1-30. doi: 10.1016/j.arabjc.2016.01.004.

Correa, J., Ortiz, D., Larrahondo, J., Sanchez, M., y Pachón, H. (2012). Actividad antioxidante en guanábana (Annona muricata); una revisión bibliográfica. Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas, 11(2), 111-126.

Delgado, L., Betanzos, G., y Sumaya, M. (2010). Importancia de los antioxidantes dietarios en la disminución del estrés oxidativo. Investigación y Ciencia, 50, 10-15.

Fernández, A. (2012). Efecto antioxidante e hipoglicemiante del extracto hidroalcohólico de las hojas de Annona muricata L. “guanábana” en Oryctolagus cuniculus “conejo” var. New Zeland. Tesis para obtención de título. Universidad Nacional de Trujillo.

Gajalakshmi. S., Vijayalakshmi, S., y Rajeswari, D. (2012). Phytochemical and pharmacological properties of Annona muricata: a review. International Journal of Pharmacy and Pharmaceutical Sciences., 4(2), 3-6.

Gavamukulya, Y., Abou-Elella, F., Wamunyokoli, F., y AEl-Shemy, H. (2014). Phytochemical screening, anti-oxidant activity and in vitro anticancer potential of ethanolic and water leaves extracts of Annona muricata (Graviola). Asian Pac J Trop Med.; 7(Suppl 1): S355-S363. doi: 10.1016/S1995-645(14)60258-3.

George, C., Kumar, D., Rajkumar, V., Suresh, P., y Ashok, K. (2012). Quantitative assessment of the relative antineoplastic potential of the n-butanolic leaf extract of Annona muricata Linn. In normal and immortalized human cell lines. Asian Pacific Journal of Cancer Prevention: APJCP, 13: 699-704. doi:http://dx.doi.org/10.7314/APJCP.2012.13.2.699.

Gyesi, J., Opoku, R., y Borquaye, L. (2019). Chemical Composition, Total Phenolic Contet, and Antioxidant Activities of the Essential Oils of the Leaves and Fruit Pulp of Annona muricata L. (Soursop) from Ghana. Biochemistry research international, 2019, 4164576. https://doi.org/10.1155/2019/4164576.

Halliwell, B., y Chirico, S. (1993). Lipid peroxidation: it´s mechanism, measurement and significance. American Journal of Clinical. Nutrition, 57(5suppl), 715S-725S. doi: 10.1093/ajcn/57.5.715S

Instituto Nacional de Salud. (2008). Guía de manejo y cuidado de animales de laboratorio: ratón. Recuperado de: https://repositorio.ins.gob.pe/xmlui/bitstream/handle/INS/117/CNPB-0002.pdf?sequence=3&isAllowed=y.

Kahkeshani, N., Saeidnia, S., y Abdollahi, M. (2015). Role of antioxidants and phytochemicals on acrylamide mitigation from food and reducing its toxicity. Journal of food science and technology, 52(6), 3169–3186. https://doi.org/10.1007/s13197-014-1558-5.

Leiva S, Gayoso G, y Chang L. (2018). Annona muricata L. "guanábana" (Annonaceae), una fruta utilizada como alimento en el Perú prehispánico. Arnaldoa.; 25(1): 127-140.

Li, S., Hong, M., Tan, H., Wang, N., y Feng, Y. (2016). Insights into the Role and Interdependence of Oxidative Stress and Inflammation in Liver Diseases. Oxidative medicine and cellular longevity, 2016, 4234061. https://doi.org/10.1155/2016/4234061.

Li, S., Tan, H., Wang, N., Zhang, Z., Lao, L., Wong, C., y Feng, Y. (2015). The Role of Oxidative Stress and Antioxidants in Liver Diseases. International journal of molecular sciences, 16(11), 26087-26124. https://doi.org/10.3390/ijms161125942.

Liguori, I., Russo, G., Curcio, F., Bulli, G., Aran, L., Della-Morte, D., Gargiulo, G., Testa, G., Cacciatore, F., Bonaduce, D., y Abete, P. (2018). Oxidative stress, aging, and diseases. Clinical interventions in aging, 13, 757–772. https://doi.org/10.2147/CIA.S158513

LoPachin, R., y Gavin, T. (2012). Molecular mechanism of acrylamide neurotoxicity: lessons learned from organic chemistry. Environmental health perspectives, 120(12), 1650–1657. https://doi.org/10.1289/ehp.1205432.

Moghadamtousi, S. Z., Kadir, H. A., Paydar, M., Rouhollahi, E., y Karimian, H. (2014). Annona muricata leaves induced apoptosis in A549 cells through mitochondrial-mediated pathway and involvement of NF-κB. BMC complementary and alternative medicine, 14, 299. https://doi.org/10.1186/1472-6882-14-299.

Nugraha, A., Damayanti, Y., Wangchuk, P., y Keller, P. (2019). Anti-infective and anti-cancer properties of the Annona species: their ethnomedicinal uses, alkaloid diversity, and pharmacological activities. Molecules (Basel, Switzerland), 24(23), 4419. https://doi.org/10.3390/molecules24234419.

Poma, E., Requis, E., Gordillo, G., y Fuertes, C. (2014). Estudio fitoquímico y actividad antiinflamatoria de la Annona muricata L. (guanábana) de Cuzco. Ciencia e Investigación, 14(2), 29-33.

Rivadeneyra-Domínguez, E., Becerra-Contreras, Y., Vázquez-Luna, A., Díaz-Sobac, R., y Rodríguez-Landa, J. (2018). Alterations of blood chemistry, hepatic and renal function, and blood cytometry in acrylamide-treated rats. Toxicology reports, 5, 1124–1128. https://doi.org/10.1016/j.toxrep.2018.11.006.

Rollin B. (2009). Ethics and euthanasia. The Canadian veterinary journal = La revue veterinaire canadienne, 50(10), 1081-1086.

Saha, S., Lee, S., Won, J., Choi, H., Kim, K., Yang, G., Dayem, A., y Cho, S. (2017). Correlation between Oxidative Stress, Nutrition, and Cancer Initiation. International journal of molecular sciences, 18(7), 1544. https://doi.org/10.3390/ijms18071544.

Saldaña-Balmori Y, Ramirez-González H, y Delgadillo-Gutiérrez H. (2003). Acción de algunos antiinflamatorios no esteroideos sobre la lipoperoxidación hepática inducida por etanol. Revista Cubana de Investigaciones Biomédicas, 22(1), 16-24.

Sánchez-Rodríguez, M., y Mendoza-Núñez, V. (2019). Oxidative Stress Indexes for Diagnosis of Health or Disease in Humans. Oxidative medicine and cellular longevity, 2019, 4128152. https://doi.org/10.1155/2019/4128152.

Singh, A., Kukreti, R., Saso, L., y Kukreti, S. (2019). Oxidative Stress: A Key Modulator in Neurodegenerative Diseases. Molecules (Basel, Switzerland), 24(8), 1583. https://doi.org/10.3390/molecules24081583.

Statistical tools for high-throughput data analysis. (25 de noviembre del 2020). Shapiro-Wilk test of normality. http://www.sthda.com/english/rsthda/shapiro-wilk.php

Sumner, S., MacNeela, J., y Fennell, T. (1992). Characterization and quantitation of urinary metabolites of [1, 2, 3-13c] acrylamide in rats and mice using carbon-13 nuclear magnetic resonance spectroscopy. Chemical Research in Toxicology, 5(1), 81–89. doi:10.1021/tx00025a014.

Torres, P., Aguilar, J., Rodríguez, A., y Pretel, O. (2007). Efecto de Mirabilis jalapa L. frente a radicales libres inducidos por Escherichia coli en Rattus rattus variedad albinus. Revista de Biología.; 10: 53-58.

Vasavada, N. (2016). One-way ANOVA (ANalysis of VAriance) with post-hoc Tukey HSD (Honestly Significant Difference) Test Calculator for comparing multiple treatments. https://astatsa.com/OneWay_Anova_with_TukeyHSD/.

Vit, P., Santiago, B., y Pérez-Pérez E. (2014). composición química y actividad antioxidante de pulpa, hoja y semilla de guanábana Annona muricata L. Interciencia,39(5), 350-353.

Zatroch, K., Knight, C., Reimer, J., y Pang, D. (2017). Refinement of intraperitoneal injection of sodium pentobarbital for euthanasia in laboratory rats (Rattus norvegicus). BMC veterinary research, 13(1), 60. https://doi.org/10.1186/s12917-017-0982-y.