Phytochemical characterization of bioactive compounds on methanolic and ethanolic leaf extracts of Myrciaria sp.

Nathalia F. Naspolini; Mariana P. Seljan; Monica C.P. Santos; Édira C.B.A. Gonçalves

doi:10.17268/sci.agropecu.2016.02.03

Autores/as

Nathalia F. Naspolini Universidade Federal do Estado do Rio de Janeiro
Mariana P. Seljan Universidade Federal do Estado do Rio de Janeiro
Monica C.P. Santos Universidade Federal do Estado do Rio de Janeiro
Édira C.B.A. Gonçalves Universidade Federal do Estado do Rio de Janeiro

DOI:

https://doi.org/10.17268/sci.agropecu.2016.02.03

Palabras clave:

Myrciaria sp, phenolic compounds, chemical profile, antioxidant capacity

Resumen

Among the native species of importance in Brazil, jabuticabeira (Myrciaria sp.) is a native fruit tree from several Brazilian regions. Few studies report the chemical constituents of the leaves and its pharmacological and nutraceutical properties. The aim of this study was to identify the phenolic compounds of the methanolic (MeOH) and ethanolic (EtOH) leaf extracts of Myrciaria sp. Phytochemical profile of the extracts was carried-out using High Performance Liquid Chromatography (HPLC) analysis. Antioxidant potential was evaluated by radical scavenging capacity with 2,2-diphenyl-1-picryl-hydrazyl (DPPH) and total phenolics were determined with Folin-Ciocalteau reagent. A total of nine different compounds were identified in the free and bound phenolics extractions: 2,4 dihydroxybenzoic, vanillin, p-coumaric, ferulic, sinapinic, rutin, epicatechin, trans-caffeic and myricetin. The extracts demonstrated high radical scavenging capacity (MeOH: 1.83 and EtOH: 8.05 mg/mL) and high phenolic content (MeOH: 1.15; and EtOH: 1.04 mg/g dry matter). The wide variability of compounds revealed and the amount of peaks not identified, gives us a background of a potential plant matrix for further investigations in order to develop a nutraceutical agent.

Citas

Arbos, K.A.; Stevani, P.C.; Castanha, R.F. 2013. Atividade antimicrobiana, antioxidante e teor de compostos fenólicos em casca e amêndoa de frutos de manga. Rev. Ceres, Viçosa, 60(2): 161-165.

Borges, L.L.; Conceição, E.C.; Silveira, D. 2014. Active compounds and medicinal properties of Myrciaria genus. Food Chem. 153: 224-33.

Brand-Williams, W.; Cuvelier, M.E.; Berset, C. 1995. Use of a free radical method to evaluate antioxidant activity. Food Science and Technology 28(1): 25-30.

Czyżewska, U.; Siemionow, K.; Zaręba, I.; Miltyk, W. 2016. Proapoptotic activity of propolis and their components on human tongue squamous cell carcinoma cell line (CAL-27). PLoS One 11:6, e0157091.

Dhanalakshmi, C.; Janakiraman, U.; Manivasagam, T.; Thenmozhi, A.; Essa, M.M.; Kalandar, A.; Khan, M.A.; Guillemin, G.J. 2016. Vanillin attenuated behavioural impairments, neurochemical deficts, oxidative stress and apoptosis against rotenone induced rat model of parkinson's disease. Neurochem Res (In press).

Diniz, D.N.; Macêdo-Costa, M.R.; Pereira, M.S.V.; Pereira, J.V.; Higino, J.S. 2010. Efeito antifúngico in vitro do extrato da folha e do caule de Myrciaria cauliflora Berg. sobre microrganismos orais. Rev Odontol UNESP 39(3): 151-156.

Duarte, A. R.; Santos, S.C.; Seraphinb, J.C.; Ferri, P.H. 2010. Environmental influence on phenols and essential oils of Myrciaria cauliflora leaves. J. Braz. Chem. Soc. 21(9): 1672-1680.

Einbond, L.S.; Reynertsona, K.A.; Luo, X.; Basileb, M.J.; Kennelly, E.J. 2004. Anthocyanin antioxidants from edible fruits. Food Chemistry 84: 23–28.

Gomes, S.; Torres, A.G. 2016. Optimized extraction of polyphenolic antioxidant compounds from Brazil nut (Bertholletia excelsa) cake and evaluation of the polyphenol profile by HPLC. J Sci Food Agric. 96(8): 2805-2814.

Henriques, A.T.; Castro, C.M.; Gonçalves, E.D.; Corrêa, E.R.; Pizzoli, G.; Zuanazzi, J.A.S.; Antunes, L.E.C.; Raseira, M.d.C.B.; Apel, M.; Marin, R.; Limberger, R.; Trevisan, R.; Franzon, R.C. 2010. Espécies frutíferas nativas do Sul do Brasil. 124. Pelotas, RS: Empresa Brasileira de Pesquisa Agropecuária (Embrapa).

John, J.; Shahidi, F. 2010. Phenolic compounds and antioxidant activity of Brazil nut (Bertholletia excelsa). J Funct Foods 2(3): 196-209.

Li, Y.; Guo, C.; Yang, J.; Wei, J.; Xu, J.; Cheng, S. 2006. Evaluation of antioxidant properties of pomegranate peel extract in comparison with pomegranate pulp extract. Food Chemistry 96(2): 254–260.

Lucena, R.F.P.; Nascimento, V.T.; Araújo, E.d.L.; Albuquerque, U.P.d. 2008. Local uses of native plants in an area of Caatinga vegetation (Pernambuco, NE Brazil). Ethnobotany Research & Applications 6: 3-14.

Morales, P.; Barros, L.; Dias, M.I.; Santos-Buelga, C.; Ferreira, I.C.; Ramirez-Asquieri, E.; Berrios, J.e.J. 2016. Non-fermented and fermented jabuticaba (Myrciaria cauliflora Mart.) pomaces as valuable sources of functional ingredients.' Food Chem. 208: 220-227.

Parka, Y.S.; Cvikrová, M.; Martincová, O.; Ham, K.S.; Kang, S.G.; Park, Y. K.; Namiesnik, J., Rombolàe, A.D.; Jastrzebskif, Z.; Gorinstein, S. 2015. In vitro antioxidative and binding properties of phenolics in traditional, citrus and exotic fruits. Food Research International 74: 37–47.

Silambarasan, T.; Manivannan, J.; Krishna-Priya, M.; Suganya, N.; Chatterjee, S.; Raja, B. 2014. Sinapic acid prevents hypertension and cardiovascular remodeling in pharmacological model of nitric oxide inhibited rats. PLoS One 9:12, e115682.

Singleton, V.L.; Orthofer, R.; Lammela-Ranvenson, R.M. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin–Ciocalteu reagent. Methods Enzymol 299: 152-178.

Someyaa, S.; Yoshikib, Y.; Okubob, K. 2002. Antioxidant compounds from bananas (Musa cavendish). Food Chemistry 79(3): 351-354.

Souza-Moreira, T.M.; Moreira, R.R.D.; Sacramento, L.V.S.; Pietro, R.C.L.R. 2008. Histochemical, phytochemical and biological screening of Plinia cauliflora (DC.) Kausel, Myrtaceae, leaves Plinia cauliflora (DC.) Kausel, Myrtaceae, leave. Brazilian Journal of Pharmacognosy 20(1): 48-53.

Wang, W.; Bostic, T.R.; Gu, L. 2010. Antioxidant capacities, procyanidins and pigments in avocados of different strains and cultivars. Food Chemistry 122(4): 1193-1198.

Received May 17, 2016.

Accepted Jun 27, 2016.

* Corresponding author

E-mail: ediracba.analisedealimentos@unirio.br (E.C.B.A. Gonçalves).