EFECTO ANTIMICROBIANO DEL ACEITE ESENCIAL Y DEL EXTRACTO ACUOSO DE CANELA (Cinnamomum zeylanicum) SOBRE Candida albicans Y Streptococcus mutans
Resumen
El presente estudio experimental, tuvo como objetivo determinar el efecto antimicrobiano in vitro del aceite esencial y del extracto acuoso de Cinnamomum zeylanicum (canela) sobre Candida albicans y Streptococcus mutans. La muestra estuvo conformada por un total de 288 placas, en la que se evaluó la Concentración Mínima Inhibitoria (CMI) y el efecto antimicrobiano. Se trabajó con seis concentraciones para cada presentación de canela. La CMI se determinó a través del método de diluciones en tubos mientras que el efecto antimicrobiano se determinó a través del método de difusión de discos. Los resultados mostraron que la CMI del extracto acuoso y el aceite esencial de canela sobre el crecimiento de la Candida albicans, fue de 1 mg/ml. Así mismo, se halló que la CMI del extracto acuoso y del aceite esencial de la canela sobre el crecimiento del Streptococcus mutans fue 0,8 mg/ml y 1 mg/ml, respectivamente. Respecto al efecto antimicrobiano de todos los preparados sólo el aceite esencial de canela tuvo efecto antimicrobiano sobre la Candida albicans.
Palabras clave: Canela, Cinnamomun zeylanicum, Candida albican, Streptococcus mutans, efecto antimicrobiano.
ABSTRACT
The aim of the present experimental research was to determinate the antimicrobial effect in vitro of the essential oil and the aqueous extract of Cinnamomum zeylanicum (canella) over Candida albicans and Streptococcus mutans. For this was inoculated a total of 288 Petri plates to determine the minimum inhibitory concentration (MIC) and the antimicrobial effect evaluating six concentrations for the two presentations of Cinnamomum zeylanicum. The MIC was measured as Colony Forming Units per milliliter (CFU / ml) while the antimicrobial effect was determined by the disk diffusion method, making reading in millimeters (mm).The results showed that the MIC of the aqueous extract and essential oil of cinnamon on the growth of Candida albicans was of 1 mg / ml. The MIC of the aqueous extract and essential oil of cinnamon on the growth of Streptococcus mutans was 0,8 mg / ml and 1 mg / ml, respectively.
Regarding the antimicrobial effect of all preparations only the essential oil of cinnamon was antimicrobial effect on Candida albicans.
Keywords: Cinnamon, Cinnamomum zeylanicum, Candida albicans, Streptococcus mutans, antimicrobial effect.
Citas
Khan R. Antimicrobial Activity of Five Herbal Extracts Against Multi Drug Resistant (MDR) Strains of Bacteria and Fungus of Clinical Origin. Molecules 2009;14: 586-597.
Braga L, Leite A, Xavie K, Takahashi J, Bemquerer M, Chartone-Souza E. Nascimento A. Synergic interaction between pomegranate extracts and antibiotics against Staphylococcus aureus. Can. J. Microbiol. 2005;51: 541-547.
Hill A. Botánica Económica Plantas útiles y productos vegetales. 1a edición. Ediciones Omega 1965. 571
Kafaru E. Immense help formative workshop. In Essential Pharmacology; 1st Ed. Elizabeth Kafaru Publishers: Lagos, Nigeria 1994.
Somchit MN, Reezal I, Nur IE, Mutalib AR. In vitro Antimicrobial Activity of Ethanol and Water Extracts of Cassialata. J. Ethnopharmacol. 2003; 84: 1-4.
Lee SB, Cha KH, Kim SN. Altantsetseg, S.; Shatar, S.; Sarangerel, O. The Antimicrobial Activity of Essential Oil from Dracocephalum foetidum against Pathogenic Microorganisms. J. Microbiol 2007; 45: 53-57.
Ooi L, Li Y, Kam Sh, Wang H, Wong E. and Ooi V. Antimicrobial Activities of Cinnamon Oil and Cinnamaldehyde from the Chinese Medicinal Herb Cinnamomum cassia Blume. The American Journal of Chinese Medicine 2006; 34(3): 511–522
Jayaprakasha G y Jagan L Chemistry, Biogenesis, and Biological Activities of C. zeylanicum. Critical Reviews in Food Science and Nutrition 2011;51(6): 547-562
Lee R. and Balick M. Sweetwood–cinnamon and itsimportance as a spice and medicine. EXPLORE. 2005;1:61–64.
Unlu M, Ergene E, Unlu G, Zeytinoglu H, Vural N. Composition, antimicrobial activity and in vitro cytotoxicity of essential oil from Cinnamomum zeylanicum Blume (Lauraceae). Food and Chemical Toxicolog. El Sevier 2010; 48: 3274–3280
Aboaba O, Efuwape BM. Antibacterial Properties of Some Nigerian Species. Bio. Res. Comm. 2001; 13: 183-188.
Carmo ES, Lima ED, de Souza EL, de Sousa FB. Effect of Cinnamomum zeylanicum blume essential oil on the growth and morphogenesis of some potentially pathogenic Aspergillus species. Braz. J. Microbiol. 2008; 39 (1): 91–97.
Yang YC, Lee HS, Lee SH, Clark JM, Ahn YJ. Ovicidal and adulticidal activities of C. zeylanicum bark essential oil compounds and related compounds against P. humanus capitis (Anoplura: Pediculicidae). Int. J. Parasitol. 2005; 35 (14): 1595–1600.
Fichi G, Flamini G, Zaralli LJ, Perrucci S. Efficacy of anessentialoil of Cinnamomum zeylanicum against Psoroptes cuniculi. Phytomedicine. 2007; 14: 227– 231.
Marongiu B, Piras A, Porcedda S, Tuveri E, Sanjust, E, Meli M, Sollai F, Zucca P, Rescigno A. Supercritical CO2 extract of Cinnamomum zeylanicum: Chemical characterization and antityrosinase activity. J. Agr. FoodChem 2007; 55 (24): 10022–10027.
Jayaprakasha GK, Negi PS, Jena BS, Jagan Mohan Rao, L. Antioxidant and antimutajenic activities of C. zeylanicum fruit extracts. J. FoodCompos 2007; 20: 330–336.
Bakkali F, Averbeck S, Averbeck D, Zhiri A, Baudoux D, Idaomar M. Antigeno toxic effects of three essential oils in diploid yeast (Saccharomyces cerevisiae) after treatments with UVC radiation, 8-MOP plus UVA and MMS 2006; 606: 27–38.
Chen CY, Liu TZ, Chen CH, Wu CC, Cheng JT, Yiin SJ, Shih MK, Wu MJ, Chern CL Isoobtusilactone A-induced apoptosis in human hepatomaHep G2cells ismediated via increased NADPH oxidase - derived reactive oxygen species (ROS) production and the mitochondria - associated apoptotic mechanisms. Food Chem. Toxicol 2007; 45: 1268–1276.
Jacob LS, Flaitz CM, Nichols CM, Hicks MJ. Role of dentinal carious lesions in the pathogenesis of oral candidiasis in HIV infection. J Am Dent Assoc 1998; 129(2):187-94
Russell JI, McFarlane TW, Aitchison TC, Stephen KW, Burchell CK. Caries prevalence and microbiological and salivary caries activity tests in Scottish adolescents. Community Dent Oral Epidemiol 1990; 18:120-125.
Rego MA, Koga-Ito CY, Jorge AOC. Effects of oral environment stabilization procedures on counts of Candida spp. in children. Pesqui Odontol Bras 2003; 17: 322-6.
Cerqueira DF. Examining Dentinal Carious Lesions as a Predisposing Factor forthe Oral Prevalence of Candida spp. in HIV-infected Children 2007; 74:2.
Seif T. Cariología, prevención, diagnóstico y tratamiento contemporáneo de la caries dental. 1ª. Ed. Caracas, Venezuela: Ed. Amolca. 1997: 27-37.
Aneja K, Joshi R, Sharma Ch. Antimicrobial activity of Dalchini (Cinnamomum zeylanicum bark) extracts on some dental caries pathogens. Journal of Pharmacy Research. 2009; 2(9): 1387-1390.
Chao SC, Young DG, Oberg, C.J. Screening for inhibitory activity of essential oil son selected bacteria, fungi and viruses. J. Essent. Oil Res 2000; 12 (5): 639–649.
Pozzatti P, Scheid L, Spader T, Atayde M, Santurio J and Alves S. In vitro activity of essential oils extracted from plants used as spices against fluconazole-resistant and fluconazole-susceptible Candida spp. Can. J. Microbiol. 2008; 54: 950–956.
Dawson SB, Trapp RG. Bioestadística Médica. 2da. Ed. México. Editorial Manual Moderno. 1999.
Sathishkumar M, Sneha K, Won S, Cho C., Kim S, Yun Y. Cinnamon zeylanicum bark extract and powder mediated green synthesis of nano-crystalline silver particles and its bactericidal activity. Colloids and Surfaces B: Biointerfaces. El Sevier.2009; 73: 332–338.
Brooks G, Batel J, Morse S. Microbiología Médica de Jawest, Meldick y Adelberg. 16va. Ed. México: el Manual Moderno: 1999
Fani M, Kohanteb J. Inhibitory Activity of Cinnamon Zeylanicum and Eucalyptus Globulus Oils on Streptococcus Mutans, Staphylococcus Aureus, and Candida Species Isolated from Patients with Oral Infections. ShirazUnivDent J 2011;11.
Walsh SE, Maillard JY, Russel AD; Catrenich, C.E.; Charbonneau, A.L.; Bartolo, R.G. Activity and Mechanism of Action of Selected Biocidal Agents on Gram -positive and –negative Bacteria. J. Appl. Microbiol. 2003; 94: 240-247.
Vinitha M, Ballal M. In vitro anticandidal activity of Cinnamomum verum. Journal of Medical Sciences. J. Med. Sci. 2008; 8(4):425-428.
Netnapis K, Somsiri S. Postharvestantifungal activity of extracts and compounds from Cinnamomum zeylanicum, Boesenbergiapandurata and Syzygiumarom aticum against Colletotrichum gloeosporioides and Botryodiplodia theobromae. AsianJournal of Food and Agro – Industry. As. J. Food Ag – Ind 2009: 125-131.
