Capillary electrophoresis as a tool for genotyping SH3 mediated coffee leaf rust resistance

Savina A.  Gutiérrez-Calle; Rosa A.  Sánchez-Díaz; Yolanda B.  Delgado-Silva; Juan D.  Montenegro; Dina L.  Gutiérrez; Jorge L.  Maicelo-Quintana; Juan C.  Guerrero-Abad

doi:10.17268/sci.agropecu.2021.011

Autores/as

Savina A. Gutiérrez-Calle Centro Experimental La Molina. Dirección de Recursos Genéticos y Biotecnología. Instituto Nacional de Innovación Agraria (INIA). Av. La Molina 1981, 15024 Lima
Rosa A. Sánchez-Díaz Departamento de Fitotecnia. Facultad de Agronomía. Universidad Nacional Agraria La Molina, La Molina. Av. La Molina s/n, 15024 Lima.
Yolanda B. Delgado-Silva Centro Experimental La Molina. Dirección de Recursos Genéticos y Biotecnología. Instituto Nacional de Innovación Agraria (INIA). Av. La Molina 1981, 15024 Lima
Juan D. Montenegro Centro Experimental La Molina. Dirección de Recursos Genéticos y Biotecnología. Instituto Nacional de Innovación Agraria (INIA). Av. La Molina 1981, 15024 Lima.
Dina L. Gutiérrez Centro Experimental La Molina. Dirección de Recursos Genéticos y Biotecnología. Instituto Nacional de Innovación Agraria (INIA). Av. La Molina 1981, 15024 Lima
Jorge L. Maicelo-Quintana Centro Experimental La Molina. Dirección de Recursos Genéticos y Biotecnología. Instituto Nacional de Innovación Agraria (INIA). Av. La Molina 1981, 15024 Lima.
Juan C. Guerrero-Abad Centro Experimental La Molina. Dirección de Recursos Genéticos y Biotecnología. Instituto Nacional de Innovación Agraria (INIA). Av. La Molina 1981, 15024 Lima.

DOI:

https://doi.org/10.17268/sci.agropecu.2021.011

Palabras clave:

coffee, capillary, electrophoresis;, Hemileia vastatrix, SH3

Resumen

Coffee is an important agricultural commodity in the world. However, it is susceptible to Hemileia vastatrix (Hv), an obligatory biotrophic fungus that causes coffee leaf rust (CLR). Natural resistance to rust has been identified in the wild species Coffea canephora and Coffea liberica. These species have been used in breeding programs where interspecific resistant hybrids have been generated. The S_H3 gene, derived from C. liberica, has been shown to confer extreme and long-lasting resistance to Hv. A total of 167 accessions of the INIA’s Coffee Germplasm Collection of Peru (INIA-CGC) were screened with 4 markers linked to the S_H3 gene. As positive controls, EA67 (C. liberica) and the hybrid S.288 (C. arabica x C. liberica) were used. Separation of PCR products was done by capillary electrophoresis, which allow to discriminate the alleles of each marker. For three markers, specific alleles for either C. arabica or C. liberica species were found. In all cases, S.288 exhibited specific alleles for both species; whereas the INIA-CGC accessions had exclusively C. arabica alleles and EA67 had C. liberica alleles. The BA-48-21O-f marker did not produce PCR fragments for any of the positive controls, suggesting that this marker is not as predictive as the other three to determine the presence of S_H3. This work reports the existence of multiple alleles for the Sat244 marker; however, the collection does not have the S_H3 mediated-resistance gene. Finally, the utility of capillary electrophoresis as a tool to identify alleles linked to S_H3 was demonstrated.

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