Early detection of resistance to Mycosphaerella fijiensis in local genotypes of Musa in Ecuador

Galo Cedeño García, Carmen Suarez Capello, Danilo Vera Coello, Carlo Fadda, Devra Jarvis, Paola de Santis

Abstract


The aim of this research was to determine resistance reaction of 14 Musa genotypes against Mycosphaerella fijensis, causal agent of Black Sigatoka disease. Two experiments were conducted in laboratory and shadehause at Pichilingue research Station from the National Institute of Agricultural Research (INIAP), Ecuador. Genotypes considered were: Orito (AA), Guineo de jardín, Williams, Filipino, Gross Mitchel, Mulato and Morado, (AAA); Limeño, Maqueño, Dominico, Dominico-Hartón, Dominico gigante, Dominico negro and Barraganete from the AAB genome. Two inoculation methods were used to asses’ resistance: a) in vitro inoculation over leaf fragments with a conidial suspension, and b) inoculation of eight weeks old seedlings with a mycelial suspension of the fungus. In the first case, the incubation period (IP) and the number of lesions at 14 and 20 days after inoculation (DDI) were measured. For in vitro trial, the incubation period (IP) and the number of leaf lesions 14 and 20 days after inoculation (DDI) were measured, and for seedlings, PI, period of symptoms evolution (TES) and time for disease development (TDE) were recorded. Statistically significant (p ≤ 0.05) differences were found for all variables. Cultivars Orito (AA) and Limeño (AAB) qualify as resistant while all the others were susceptible, though response to inoculation within the latter group showed a range of variability.

Keywords


Musa cultivars; inoculation; inoculum; resistance; biodiversity

References


Abadie, C.; Zapater, M.; Pignolet, L.; Carlier, J.; Mourichon, X. 2008. Artificial inoculation on plants and banana leaf pieces with Mycosphaerella spp., responsible for Sigatoka leaf spot diseases. Fruits 63(5): 319 – 323.

Acosta, M.; Alvarado, Y.; Cruz, M.; Leiva, M.; Roque, B. 2004. Evaluación en casa de cultivo de la respuesta a la Sigatoka negra de dos cultivares de Musa mediante la inoculación artificial de suspensiones conidiales de Pseudocercospora fijiensis. Biotecnología Vegetal 4(2): 77 – 84.

Aguilar, A.; García, A.; Odrizola, O.; Macedo, G.; Ogura, T.; Manzo, G.; James, A.; Islas, I.; Beltrán, M. 2014. Chemical management in fungicide sensivity of Mycosphaerella fijiensis collectedfrom banana fields in México. Brazilian Journal of Microbiology 45(1): 359 – 364.

Aguirre, S.; Piraneque, N.; Rodríguez, J. 2015. Relationship between the nutritional status of banana plants and black sigatoka severity in the Magdalena region of Colombia. Agronomía Colombiana 33(3): 348 – 355.

Alvarado, Y.; Leiva, M.; Dita, M.; Acosta, M.; Cruz, M.; Portal, N.; Gómez, R.; García, L.; Bermúdez, I.; Padrón, J. 2003. Early Evaluation of black leaf streak resistance by using mycelial suspensions of Mycosphaerella fijiensis. In: Jacome L., Lepoivre P., Marin D., Ortiz R., Romero R., and Escalant J.V. Mycosphaerella leaf spot diseases of bananas: present status and outlook. Montpellier, France, INIBAP.

Álvarez, E.; Pantoja, A.; Gañán, L.; Ceballos, G. 2013. Estado del arte y opciones de manejo del Moko y la Sigatoka negra en América Latina y el Caribe. CIAT – FAO. Cali, Colombia. 40 pp.

Amari, E.; Dick, E.; Kone, D.; Abo, K.; Kobenan, K. 2011. Response to juglone toxic effect in various genotypes of banana (Musa AA, AAA, AAB, AAAA, AAAB). Agronomie Africaine 23(3): 247 – 258.

Altieri, M.; Nicholls, C. 2004. Biodiversity and pest management in agroecosystems 2nd ed. Binghamton, USA. Food Products Press.

Arango, R.; Diaz, C.; Dhillon, B.; Aerts, A.; Carlier, J.; Crane, C.; de Jong, T.; de Vries, I.; Dietrich, R.; Farmer, A.; Fortes, C.; García, S.; Guzmán, S.; Hamelin, R.; Lindquist, E.; Mehrabi, R.; Quiros, O.; Schmutz, J.; Shapiro, H.; Reynolds, E.; Scalliet, G.; Souza, M.; Stergiopoulus, I.; Van der Lee, T.; De wit, P.; Zapater, M.; Zwiers, L.; Grigoriev, I.; Goodwin, S.; Kema, G. 2016. Combating a Global Threat to a Clonal Crop: Banana Black Sigatoka Pathogen Pseudocercospora fijiensis (Synonym Mycosphaerella fijiensis) Genomes Reveal Clues for Disease Control. PLoS Genet 12(8): 1 – 36.

Beltrán, M.; Prado, F.; Oliveira, M.; Ortíz, D.; Scalfo, A.; Pessoa, A.; Medeiros, M.; White, J.; Di Mascio, P. 2014. Singlet Molecular Oxygen Generation by Light-Activated DHN-Melanin of the Fungal Pathogen Mycosphaerella fijiensis in Black Sigatoka Disease of Bananas. PLOS ONE 9(3): 1 – 15.

Castilla, N.; Vera-Cruz, C.; Mew, T. 2003. Using rice cultivars mixtures: a sustainable approach for managing diseases and increasing yield. Mini Review. IRRN 28(2): 5 – 11.

Cuéllar, A.; Álvarez, E.; Castaño, J. 2010. Evaluación de Resistencia de Genotipos de Plátano y Banano a la Sigatoka Negra (Mycosphaerella fijiensis Morelet.). Rev.Fac.Nal.Agr. Medellín 64(1): 5853-5865.

Chillet, M.; Castelan, F.; Abadie, C.; Hubert, O.; De Bellaire, L. 2013. Necrotic leaf removal, a key component of integrated management of Mycospaerella leaf spot diseases to improve the quality of banana: the case of Sigatoka disease. Fruits 68: 271 – 277.

Daniells, J. 2000. ¿Qué variedad de banano debo cultivar?. InfoMusa 9(1): 31 – 33.

Daniells, J.; Jenny, C.; Karamura, D.; Tomekpe, K. 2001. Musalogue: a catalogue of Musa germplasm. Diversity in the genus Musa (E. Arnaud and S. Sharrock, compil.). International Network for the Improvement of Banana and Plantain, Montpellier, France. 213 p.

de Langhe, E.; Hribova, E.; Carpentier, S.; Dolezel, J.; Swennen, R. 2010. Did backcrossing contribute to the origin of hybrid edible bananas. Annals of Botany 106: 849 – 857.

Drenth, A.; Guest, D. 2016. Fungal and oomycete diseases of tropical tree fruit crops. Annu. Rev. Phytopathol. 54: 373 – 395.

Divine, T., Dominic, F.; Ivo, L. 2011. Evaluation of varietal response to black Sigatoka caused by Mycosphaerella fijiensis Morelet in banana nursery. Int. Res. J. Plant Sci. 2(10): 299 – 304.

Didelot, F.; Brun, L.; Parisi, L. 2007. Effects of cultivar mixtures on scab control in apple orchards. Plant Pathology 56: 1014 – 1022.

El Handrami, E.; Kone, D.; Lepoivre, P. 2005. Effect of juglone on active oxygen species and antioxidant enzymes in susceptible and partially resistant banana cultivars to black leaf streak disease. European Journal of Plant Pathology 113(3): 241-254.

Fabiane, D., Bart, F.; Robert, M. 2015. Sigatoka Disease Complex of Banana in Brazil: Management Practices and Future Directions. Outlooks on Pest Management 26(2): 78 – 81.

Fouré, E. 1985. Black leaf streak disease of bananas and plantains (Mycosphaerella fijiensis Morelet), study of the symptoms and stages of the disease in Gabon. Irfa, Paris.

Giménez, C.; Colmenares, M. 2004. Evaluación in vitro de la resistencia a las toxinas de Mycosphaerella fijiensis en Musa spp. Ciencia 12(4): 251 – 257.

Guzmán, M.; Orosco, M.; Pérez, V. 2013. Las enfermedades Sigatoka de las hojas del banano: dispersión, impacto y evolución de las estrategias de manejo en América Latina y el Caribe. En: Memorias de la XX Reunión Internacional ACORBAT, 9 – 13 de Septiembre 2013, Fortaleza, Ceará, Brasil.

Hernández, Y.; Portillo, F.; Portillo, M.; Navarro, C.; Rodríguez, M.; Velazco, J. 2006. Densidad estomática en materiales de plátano (Musa AAB, AAAB y ABB) susceptibles y resistentes a Sigatoka Negra (Mycosphaerella fijiensis, Morelet). Rev. Fac. Agron. (LUZ) 23: 292 – 297.

Hidalgo, M.; Tapia, A.; Rodríguez, W.; Serrano, E. 2006. Efecto de la Sigatoka negra (Mycosphaerella fijiensis) sobre la fotosíntesis y transpiración foliar del banano (Musa sp. AAA, cv. Valery). Agronomía Costarricense 30(1): 35-41.

Hidalgo, W.; Chandran, J.; Menezes, R.; Otálvaro, F. and Schneider, B. 2016. Phenylphenalenones protect banana plants from infection by Mycosphaerella fijiensis and are deactivated by metabolic conversion. Plant. Cell. Environ. 39(39): 492 – 513.

Jarvis, D.; Hodgkin, T.; Sthapit, B.; Fadda, C.; López, I. 2011. An Heuristic Framework for Identifying Multiple Ways of Supporting the Conservation and Use of Traditional Crop Varieties within the Agricultural Production System. Critical Reviews in Plant Science 30: 125 – 176.

Jones D. 1993. Evaluating banana and plantain for reaction to black leaf streak disease in the South Pacific. Tropical Agriculture 70: 39-44.

Kumakech, A.; Lyngs, H.; Edema, R.; Okori, P. 2015. Efficient screening procedure for black Sigatoka disease of banana. African Crop Science Journal 23(4): 387 – 397.

Leiva, M.; Dita, M.; Alvarado, Y.; Acosta, M.; García, L.; Bermúdez, I. 2002. Empleo de diferentes inóculos de Mycosphaerella fijiensis Morelet en condiciones de invernadero para evaluar el comportamiento de dos cultivares de banano. InfoMusa 11(2): 41 – 42.

Leiva, M.; Alvarado, Y.; Acosta, M.; Cruz, M.; Sánchez, C.; Roque, B. 2010. Protocolo para la inoculación artificial de plantas de Musa spp. con Mycosphaerella fijiensis y evaluación de su respuesta mediante variables epifitiológicas y componentes de resistencia. Biotecnología vegetal 10(2): 79 – 88.

Leiva, M.; Alvarado, Y.; Acosta, M.; Cruz, M.; Roque, B.; Mena, E. 2015. Components of resistance to assess Black Sigatoka response in artificially inoculated Musa genotypes Rev. Protección Vegetal 30: 60 – 69.

Marcano, I.; Díaz, C.; Seco, V.; Urbano, B.; González, F. 2016. Induced Systemic Resistance Could Explain the Reduction in the Incidence of Black Sigatoka (Mycosphaerella fijiensis) in Banana Plants Inoculated with Bacteria Isolated from Banana Tree Roots in the Dominican Republic. In: González-Andrés F., James E. (eds) Biological Nitrogen Fixation and Beneficial Plant-Microbe Interaction. Springer, Cham.

Marcillo, J. 2014. Contribución económica de la biodiversidad intraespecífica: Caso Musas spp., en El Carmen y La Maná a nivel del pequeño productor. Editora Académica Española, Madrid, España. 192 pp.

Mourichon, X.; Lepoivre, P.; Carlier, J. 2000. Host – Pathogens interactions. Chapter 2. Fungal disease of follaje. In: disease of banana. 67 – 72 pp.

Molina, O.; Castaño, J. 2003. Análisis de algunos componentes de resistencia en los híbridos de banano y plátano FHIA 01, FHIA 17 y FHIA 21 a las Sigatokas negra (Mycosphaerella fijiensis Morelet) y amarilla (M. musicola Leach). Rev. Acad. Colomb. Cienc. 27(103): 181 – 190.

Mulumba, J.; Nankya, R.; Adokorach, J.; Kiwuka, C.; Fadda, C.; De Santis, P.; Jarvis, D. 2012. A risk-minimizing argument for traditional crop varietal diversity use to reduce pest and disease damage in agricultural ecosystems of Uganda. Agriculture, Ecosystems and Environment 157: 70 – 86.

Noar, R.; Daub, M. 2016a. Transcriptome sequencing of Mycosphaerella fijiensis during association with Musa acuminata reveals candidate pathogenicity genes. BMC Genomics 17(690): 1 – 25.

Noar, R.; Daub, M. 2016b. Bioinformatics Prediction of Polyketide Synthase Gene Clusters from Mycosphaerella fijiensis. PLoS ONE 11(7): 1 – 31.

Panissi, E. 2010. Armed and dangerous. Science 327: 804-805.

Pérez, L.; Pérez, M.; Jiménez, M.; Jama, M. 2006a. Ensayo en fragmentos de hojas de bananos y plátanos (Musa spp.) para el estudio a nivel monocíclico de la evolución de los síntomas de la Sigatoka negra causada por Mycosphaerella fijiensis Morelet. Fitosanidad 10(1): 3 – 9.

Pérez, M.; Pérez, L.; Trujillo, R.; Betancourt, D. 2006b. Variabilidad de Mycosphaerella fijiensis Morelet., estabilidad de la resistencia a Sigatoka negra de los clones híbridos de la FHIA. Fitosanidad 10: 37 – 47.

Pérez, L. 2013. Estrategias para reducir el uso de agroquímicos en bananos y plátanos. En: Memorias de la XX Reunión Internacional ACORBAT, 9 – 13 de Septiembre 2013, Fortaleza, Ceará, Brasil.

Ploetz, R.; Kepler, A.; Daniells, J.; Nelson, S. 2007. Banana and plantain—an overview with emphasis on Pacific island cultivars, ver. 1. In: Elevitch, C.R. (ed.). Species Profiles for Pacific Island Agroforestry. Permanent Agriculture Resources (PAR), Hōlualoa, Hawai‘i. Disponible en: http://www.ctahr.hawaii.edu/sustainag/extn_pub/fruitpubs/Banana-plantain-overview.pdf

Rodríguez, A.; Cayón, G. 2008. Efecto de Mycosphaerella fijiensis sobre la fisiología de la hoja de banano. Agronomía Colombiana 26(2): 256-265.

Rodríguez, C.; Cayón, D.; Mira, J. 2012. Effect of Number of Functional Leaves at Flowering on Yield of Banana Grand Naine (Musa AAA Simmonds). Rev. Fac. Nal. Agr. Medellín 65(2): 6585-6591.

Sánchez, C.; Alvarado, Y.; Acosta, M.; Cruz, M.; Leiva, M.; Roque, B. 2010. Relación entre caracteres fenotípicos de plantas de ‘Grande Naine’ y ‘Calcutta 4’ cultivadas in vitro y su respuesta a la inoculación con Mycosphaerella fijiensis en casa de cultivo. Biotecnología Vegetal 10(1): 21 – 30.

Sánchez, E.; Hidalgo, L.; Pacheco, R.; Chávez, T., Navarrete, O.; Santos, E. 2016. Identification of differentially-expressed genes in response to Mycosphaerella fijiensis in the resistant Musa accession ‘Calcutta-4’ using suppression subtractive hybridization. PLoS ONE 11(8): 1 – 17.

Sagratzki, P.; Eiji, R.; Gasparotto, L.; Albuquerque, R.; Teodoro de Souza, J. 2015. Biological control of banana black Sigatoka disease with Trichoderma. Ciência Rural 45(6): 951 – 957.

Silveira, L.; Bibiano, L.; Ferreira, G.; Eiji, R.; Mizubuti, E. 2014. Baseline sensitivity of Brazilian Mycosphaerella fijiensis isolates to protectant and systemic fungicides. Tropical Plant Pathology 39(2): 172 – 177.

Torres, J.; Rodríguez, H.; Rodríguez, E.; Arango, R. 2009. Aspectos bioquímicos de la resistencia del banano (Musa acuminata) al ataque del hongo Mycosphaerella fijiensis Morelet. Tumbaga 4: 85 – 96.

Tuo Seydou.; Amari, G.; Chérif, M.; Ouédraogo, L.; Kassi, F.; Kouamé, G.; Camara, B.; Koné, D. 2017. Agronomic Performance of Plantain Cultivars (Musa spp.) in Efficient Mixing Situation for the Control of Black Sigatoka in Southern Côte d’Ivoire. Asian. J. Plant. Pathol. 11: 1 – 9.

Valerio, R.; Lindorf, H.; de García, E. 2002. Anatomía foliar comparada de ocho cultivares de banano con relación a la resistencia o susceptibilidad a la Sigatoka (amarilla y negra). Agronomía Tropical 52(4): 507 – 521.

Vera, D. 2003. Componentes epidemiológicos e progresso da Sigatoka negra em bananeria e bananeria-da-terra. Tese MS. Universidade Federal de Viçosa. 49 p.

Zhu, Y.; Chen, H.; Fan, J.; Wang, Y.; Li, Y.; Chen, J.; Fan, J.; Yang, S.; Hu, L.; Leung, H.; Mew, T.; Teng, P.; Wang, Z.; Mundt, C. 2000. Genetic diversity and disease control in rice. Nature 406: 718 – 722.

Received October 17, 2016.

Accepted March 06, 2017.

Corresponding author: alex.musaespam@gmail.com (G. Cedeño).




DOI: http://dx.doi.org/10.17268/sci.agropecu.2017.01.03

Refbacks

  • There are currently no refbacks.


Licencia de Creative Commons Scientia Agropecuaria by Universidad Nacional de Trujillo is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License.